Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"ATPS"”.
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Investigation into the formation of a protein-protein complex between ATP sulfurylase and APS reductase in onion (Allium cepa L.) : a thesis presented in partial fulfilment of the requirements for the degree of Masters in Science in Plant Biology at Massey University, Palmerston North, New Zealand
… (APSR; EC 1.8.4.9) and ATP-sulfiirylase (ATPS; EC 2.7.7.4) of Allium cepa (onion). Genes encoding APSR and ATPS from onion were expressed in E. coli, and the recombinant proteins were expressed and purified with glutathione Sepharose 4B chromatography and ion exchange chromatography. …
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Improving an aqueous two-phase process for C-phycocyanin extraction from Spirulina
… in literature is aqueous twophase separation (ATPS), which is able to produce high purity C-PC with good recovery. At the University of Cape Town (UCT), the Centre for Bioprocess Engineering Research (CeBER) has patented a process for extracting and purifying C-PC from Spirulina using a …
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Optimization of sequential purification of beta-glucosidase from tricoderma reesei in aqueous two-phase system
… by separation with an aqueous two-phase system (ATPS), was implemented for the purification of beta-glucosidase from the culture filtrate of T. reesei. The cultivation medium (nutrient) was optimized for the production of betaglucosidase from T. reesei cell culture. Treatment of the crude extract …
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The Need For Academic Reform In Vietnamese Higher Education (VHE) To Align With Employers’ Expectations
… an analysis of the advanced training programs (ATPs), the author argues that the wider use and expansion of ATPs is the key to the academic reform necessary to meet the expectations of Vietnamese employers in the 21st century.</p>
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ABC terpolymers : micelles, polymersomes and stabilisation of water in water emulsions
… properties of aqueous two phase systems (ATPSs), which are able to provide w/w emulsions, with templated self-assembly of polymersomes. This novel method provides high encapsulation efficiencies of payload species which is effective, scalable and biocompatible.This work started by design …
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Diseño de un sistema de dos fases acuoso para la recuperación de células madre a partir de tejido adiposo y escalamiento en biorreactor para su producción y uso en terapias regenerativas alogénicas
… de los sistemas de dos fases acuosos (ATPS) en la purificación de MSC a partir de muestras de lipoaspirado (ASC) debido a su alta biocompatibilidad, bajo costo y fácil escalabilidad. En cuanto a la producción, se comparó la expansión de iPSC en microcarriers y en agregados celulares, en …
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Purification of C-phycocyanin from Spirulina - adsorption pretreatment options
… a process using aqueous two-phase separation (ATPS) and ammonium sulfate precipitation to generate C-PC for cosmetic use, which has a minimum purity requirement of 1.5. A patent was obtained before further work was conducted on this process to optimise certain aspects of it. Following on from …
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The Study of 3D Micro-fluid Printing in Aqueous Two-phase System
<p>Aqueous two-phase system (ATPS) is a technology that allows phase separation in an aqueous environment. This technology can be used for the separation, extraction and purification of a variety of biomolecules and thus gained great attention in industry and academia. This thesis is a combination …
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Monoclonal antibody (mAb) purification by counter current chromatography (CCC)
… cell culture, using aqueous two-phase systems (ATPS) of inorganic salts and polymer. A polyethylene glycol (PEG) and sodium citrate system was found to be the most appropriate by robotic phase system selection. The search for an economical alternative to protein A HPLC is a substantial …
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Countercurrent chromatography of proteins using aqueous two-phase systems
… phase systems. Aqueous two - phase systems (ATPS) have been used historically to purify bio-molecules whilst retaining their biological function. This thesis focuses on extending CCC to the separation of proteins, using a model ATPS of PEG-1000 and potassium dihydrogen phosphate to separate a …
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Control of HslUV protease function by nucleotide binding and hydrolysis
… that HslU in solution is only able to bind 3-4 ATPs at saturation. This result rules out a model of ATP hydrolysis in which six nucleotides bind and are hydrolyzed together in a single power stroke and also suggests that many HslU crystal structures represent states that are not populated in the …
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Troubleshooting, optimization and robustness testing of CeBER process for c-phycocyanin extraction and purification from spirulina
… recovery overall. It was found that both of the ATPS systems investigated by previous CeBER researchers did not perform sufficiently robustly when required to handle a variation in the Spirulina source and hence crude extract feed. As such, the ATPS steps was deemed unsuitable for use in a …
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Active Learning Within Athletic Training Education
… from the CAATE website. Findings concluded that ATPs that implemented active learning within their departments had much higher TPI scores, but these programs were not as prevalent as those with lower TPI scores. Furthermore, no significant relationship was demonstrated between active learning …
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Techno-Economic Evaluation of a Commercial c-Phycocyanin Extraction Process
… using a PEG-MDX aqueous two-phase separation (ATPS) and an ammonium sulphate precipitation train before the product was dried. Process 2 was an extension of the base process design based on work done by Hockey (2022) and included an additional citrate-PEG ATPS at the start of the purification …
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Engineering a Photo-Control Mechanism for RNA Interference
… the corresponding free nucleoside. Base-caged ATPs were inactive until photoexposure to 365nm light. Additionally, these compounds did not demonstrate competitive inhibition of protein-ATP interactions, as previously described for γ-phosphate caged ATP. For the site-specific incorporation of …
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Enantioseparation using a counter-current bioreactor
… could be separated. Aqueous two-phase systems (ATPS) were the major two-phase systems used to provide SP and MP, as these are well reported to be effective in preserving enzyme activity. The distribution ratios of DL-ABA, KBA and DAAO were measured in a range of phases with polyethylene glycols …
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POLYELECTROLYTE NANOPARTICLE MEMBRANES AT WATER-WATER INTERFACES
… (PEs) on aqueous two-phase systems (ATPSs) provides a scalable route to assemble functional, stimuli-responsive membranes. The aim of this thesis is to identify the membrane self-assembly mechanism of such systems. We study two PE/NP systems in a dextran/PEG ATPS and find very …
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Entwicklung und Untersuchung eines Verfahrens zur integrierten Aufreinigung von Plasmid DNA mittels wässriger Zweiphasenextraktion
… DNA followed by selective partitioning in an ATPS. During this procedure, chromosomal and open circular DNA are irreversibly denatured. The drastic change of hydrophobicity enabled the separation from native supercoiled plasmid DNA using the second aqueous two phase system. With this …
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Self-assembly behavior in hydrophilic block copolymers
… to conclude that aqueous two-phase systems (ATPS) is a valid source of inspiration to conceive DHBCs capable of self-assembling. In the second part the principle was extended to polypeptide systems with the synthesis of a poly(N-hydroxyethylglutamine)-block-poly(ethylene glycol) copolymer. …
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Genetic and epigenetic variation within extracellular matrix genes as risk factors for human tendinopathy
… and ECM regulatory enzymes are associated with ATPs in Caucasians. The findings of this thesis should to be replicated in new and larger cohorts from different ethnic backgrounds before being incorporated into multifactorial risk assessment models aiming at reducing the incidence of human …