Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 124 for “"ATP-dependent"”.
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DYNAMICS OF NUCLEOSOME REMODELING BY ATP-DEPENDENT CHROMATIN REMODELERS
… regulating the dynamic nature of chromatin are ATP-dependent chromatin remodelers- ISWI, SWI/SNF, INO80, and CHD/Mi2. All chromatin remodelers transduce the energy from ATP hydrolysis to translocate on DNA, break histone-DNA contacts, and mobilize nucleosomes. However, the final outcomes of …
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Developing as assay to screen inhibitors for various ATP-dependent ligases
… I in the presence of doxorubicin (inhibitor of ATP-dependent ligases). These results when analysed in parallel between the conventional electrophoretic assay and the labelled nick-sealing assay showed that the newly developed assay is a reliable non-electrophoretic method in identifying potent …
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Structural and functional studies of the ATP-dependent Clp proteases in cyanobacteria
… of which one major contributor is the AAA+ (ATPases Associated with diverse cellular Activities) family that includes the Clp proteases. The Clp protease exists in many forms of life, ranging from eubacteria to mammals. In the bacterium E. coli, the hexameric ATPases ClpX and ClpA recognize …
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Function of the ATP-dependent Metalloprotease FtsH during sporulation in Bacillus subtilis
The analysis of the function of the ftsH gene of Bacillus subtilis started about ten years ago. It was shown at that time that an ftsH knockout was viable, but exhibited a pleiotropic phenotype. Cells are sensitive to salt and heat shock, exhibit filamentous growth, are difficult to be transformed …
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Mechanisms and regulation of ATP-dependent, steady state actin subunit exchange: Concurrent treadmilling & dynamic instability
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01
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Characterisation of the ATP-dependent ligase, Lig E: An investigation into its significance in Neisseria gonorrhoeae
… ligase, N. gonorrhoeae expresses a minimal ATP-dependent ligase, Ligase E (Lig E), which contains a signal peptide that indicates a likely periplasmic location as opposed to the cytosolic location of genomic DNA. Hence, I hypothesise that Lig E is working on repairing fragmented …
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THE UNIQUE STRUCTURE AND MECHANISM OF INO80 - AN ATP DEPENDENT REMODELER OF THE HISTONE EXCHANGER FAMILY
… of INO80 from the canonical model of how ATP-dependent remodelers interact and mobilize nucleosome is apparently due to its unique role as a member of the remodeling complexes that promote the exchange of H2A/H2B dimer from core nucleosome particle.
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The role of the SWI/SNF ATP dependent chromatin remodelling complex in the regulation of the human hair follicle cell proliferation and control of the human cutaneous wound healing
… modifications, nucleosome positioning and ATP-dependent chromatin remodelling as well as higher order chromatin folding and 3D genome organisation. ATP-dependent chromatin remodelling complexes modulate nucleosome structure, positioning and chromatin de-compaction and are involved in gene …
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Analysis of CHD Remodelers during Development: A Tale in Two Organisms
… transitions. Organisms have recruited ATP-dependent chromatin remodelers to ensure the correct timing of gene expression during developmental transitions. Here I show how different CHD ATP-dependent chromatin remodelers regulate developmental transitions of different organisms. I show …
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Examination of Glucose Transport in Clostridium Beijerinckii NCIMB 8052 and the Solvent Hyper-Producing Mutant BA101
… the presence of a phosphoenolpyruvate (PEP) dependent phosphotransferase system (PTS) associated with cell-free extracts of C. beijerinckii BA101 by glucose phosphorylation by PEP. The PTS activity associated with C. beijerinckii BA101 was 50% of that observed for C. beijerinckii 8052. C. …
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Structural Investigation of the Chromatin Remodeller Fun30
… of proteins and protein complexes including ATP-dependent remodelling enzymes related to the yeast Snf2 protein. A major factor limiting insight into ATP- dependent chromatin remodelling enzymes is a lack of structural understanding. The <i>Saccharomyces cerevisiae </i>Snf2 family members …
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Propionyl holocarboxylase synthesis
… livers of biotin-deficient rats, catalyzes the ATP-dependent synthesis of propionyl holocarboxylase from d-biotin and propionyl apocarboxylase. This system has been resolved by alumina C<sub>∂</sub> gel fractionation into two essential components; (a) gel supernatant which contains propionyl …
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ClpX interactions with ClpP, SspB, protein substrate and nucleotide
ClpXP and related ATP-dependent proteases are implements of cytosolic protein destruction. They couple chemical energy, derived from ATP hydrolysis, to the selection, unfolding, and degradation of protein substrates with the appropriate degradation signals. The ClpX component of ClpXP is a …
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Investigating the mechanisms of action of the SNF2-homolog protein Fun30
… which this can be achieved is via the action of ATP-dependent chromatin remodelling complexes related to the S.cerevisiae Snf2 proteins. The Fun30 protein (Function unknown now 30) shares sequence homology with the Snf2 family of chromatin remodelling proteins. Here the activities and roles of …
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Local and global investigations into DEAD-box protein function
… functional conformations. DEAD-box proteins are ATP-dependent RNA chaperone proteins that function by disrupting structure, which can facilitate structural conversions. Here, studies with both local and global focuses are used to uncover mechanistic features of DEAD-box proteins CYT-19 and …
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The Synthesis of Oligodeoxyribonucleotides With Rna Ligase
… T4-infected Escherichia coli catalyzes the ATP-dependent formation of a 3' (--->) 5'-phosphodiester bond between one oligonucleotide with a 3'-hydroxyl group (the acceptor molecule) and another with a 5'-phosphate (the donor molecule). The enzyme does not require the alignment of the donor …
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The N-end rule degradation pathway : substrate recognition and staged delivery to the CIpAP protease by the CIpS adaptor protein
… all organisms, ranging from bacteria to mammals, ATP-dependent proteases carry out regulated protein degradation in order to maintain homeostasis as well as to respond to stress. ATP-dependent proteases are responsible for the degradation of a broad array of substrates and for that reason, a high …
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Measurement and Analysis of Calcium-Dependent Exocytosis in Giant Excised Membrane Patches
Ca2+-dependent exocytosis was studied in both excised and whole-cell patch clamp with emphasis on the rat secretory cell line, RBL. Capacitance and amperometric recordings show that secretory granules (SGs) containing serotonin are mostly lost from excised patches. Small vesicles that are retained …
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Glutathione synthetase: Conserved residues of the substrate loop
… glutathione (GSH) is synthesized in two ATP-dependent steps; the second enzyme in the biosynthetic pathway, glutathione synthetase (GS), ligates glycine to γ-glutamylcysteine (γ-GC). Human glutathione synthetase (hGS) deficiency causes hemolytic anemia, metabolic acidosis, 5-oxprolinuria …
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Metals in Chemistry and Biology: Computational Chemistry Studies
… synthetase (GS), an enzyme that belongs to the ATP-grasp superfamily, catalyzes the (Mg, ATP)-dependent biosynthesis of glutathione (GSH) from γ-glutamylcysteine and glycine. The free and reactant forms of human GS (wild-type and glycine mutants) were modeled computationally by employing …
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