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Showing 1 to 20 of 78 for “"ATP synthase"”.
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Studies of the assembly pathway of human ATP synthase
Human mitochondrial ATP synthase is an enzyme containing 18 unlike subunits located in the inner mitochondrial membrane (IMM), where the catalytic F1 domain extends into the mitochondrial matrix and the FO domain, which contains the c8-ring rotor, the a-subunit and the supernumerary subunits, is …
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Inhibition of <em>Escherichia coli</em> ATP Synthase Using Bioflavonoids.
<p>ATP synthase is the fundamental means of the cellular energy production in all organisms. Malfunction of ATP synthase is associated with multiple disease conditions. This enzyme is not only implicated in disease conditions but also likely to contribute in new therapies for multiple diseases by …
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Investigating the Rotary Mechanism of ATP Synthase Using Molecular Dynamics Simulations
F1-ATPase is a motor protein that can use ATP hydrolysis to drive rotation of the central subunit. The γ C-terminal helix constitutes of the rotor tip that is seated in an apical bearing formed by the α3β3 head. It remains uncertain to what extent the γ conformation during rotation differs from …
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Struktur der ATP-Synthase - Elektronenmikroskopische Untersuchungen an zweidimensionalen Kristallen und Einzelteilchen
Die ATPsynthase aus Chloroplasten ist ein membrangebundenes Enzym, das die ATPsynthese/hydrolyse an einen transmembranen Protonentransport koppelt. In dieser Arbeit wurden strukturelle Untersuchungen an der ATPsynthase aus Chloroplasten mit Hilfe der Elektronenmikroskopie und digitalen …
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Charakterisierung der Untereinheit e der mitochondrialen F1FO-ATP Synthase aus Saccharomyces cerevisiae
… Untereinheit e (Sue) der mitochondrialen F1FO-ATP Synthase von Saccharomyces cerevisi-ae. Anhand der Resultate der durchgeführten Experimente wurden folgende Schlussfolgerungen gezogen: (1) Sue ist der Lage, ein Sue-Homodimeres zu bilden. Das Protein spielt eine zentrale Rolle bei der …
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Inhibition of <em>Escherichia coli</em> ATP Synthase by Polyphenols and Their Derivatives.
… polyphenols on <em>Escherichia coli</em> ATP synthase to test (I) if the beneficial dietary effects of polyphenols are related to their inhibitory actions on ATP synthase, (II) if inhibitory effects of polyphenolic compound could be augmented through structural modifications, and (III) if …
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Effects of loigomycin, F0/F1 ATP-synthase inhibitor on mitochondrial morphology and dynamics
… the cells die. We found that the intracellular ATP level reduced by oligomycin did not affect on the mitochondrial fission, but high ROS level produced in early stage modulate mitochondrial fission machinery, which is evidenced by blocking of the fission with antioxidants. Finally, the early …
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Effects of Changes of Redox Properties of the Chloroplast ATP Synthase in Arabidopsis
… In the isolated mutant chloroplasts, the high ATPase activities did not require DTT treatment while in the wild-type plant, ATPase activities increased after DTT treatment indicating that the cysteine to serine substitutions caused loss of the redox regulation and the ATP synthases were locked …
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Structural and biochemical studies of the regulation and catalytic mechanism of ATP synthase
ATP synthase (F1Fo-ATPase) catalyses the production of ATP from ADP and orthophosphate by using the proton motive force established across a membrane by photosynthesis or oxidative phosphorylation. The ATP synthase of eukaryotic mitochondria is located in the inner membrane and is comprised of two …
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Three-Dimensional (3D) pharmacophore-based identification of possible ATP-synthase inhibitors for mycobacterium tuberculosis (Mtb)
… aimed at inhibiting the adenosine triphosphate (ATP) synthase of MDR-TB, thereby targeting the energy metabolism mechanism of Mtb. Drug regimens utilized prior to BDQ have shown low success rates in patients with XDR-TB. The technologies of computer-aided drug discovery (CADD) has proven to be a …
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Intrinsic uncoupling in the ATP synthase of Escherichia coli. Studies on WT and ε-truncated mutants
The H+/ATP ratio in the catalysis of ATP synthase has generally been considered a fixed parameter. However, Melandri and coworkers have recently shown that, in the ATP synthase of the photosynthetic bacterium Rb.capsulatus, this ratio can significantly decrease during ATP hydrolysis when the …
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Molecular Dynamics Simulations and Cross-Link Analysis of the Rotary Molecular Motor Fo of ATP Synthase
Submitted by Meng Tao (mengtao2@illinois.edu) on 2012-10-23T18:32:04Z No. of bitstreams: 1 Kanchanawarin_Chalermpol.pdf: 331004154 bytes, checksum: 79e6ed6e3fe4628353c529ad121de6ec (MD5)
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Molecular Dynamics Simulations and Cross-Link Analysis of the Rotary Molecular Motor F(o) of ATP Synthase
The protein F1Fo ATP synthase is responsible for the generation of the molecule adenosine tri-phosphate (ATP). It consists of two coupled rotary motors, F1 and Fo. ATP is generated via a rotary mechanism that couples the synthesis of ATP in F1 to the proton translocation across Fo which is driven …
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Properties of Conductance and Inhibition of Proton Channels: M2 from Influenza A Virus and Fo from Escherichia coli ATP Synthase
… viral RNA release. The proton-translocating FoF1 ATPase (ATP synthase) uses a proton gradient to drive adenosine triphosphate (ATP) synthesis. We have directly measured proton uptake in vesicles containing reconstituted M2 or FO by monitoring external pH after addition of valinomycin to vesicles …
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The ATP synthase from spinach chloroplasts (Spinacea oleracea) : improved isolation and purification of the enzyme and conformational changes observed with fluorescent labels
The ATP Synthase from Chloroplasts, CF0F1, is an integral membrane enzyme, which catalyses the synthesis of ATP from ADP and Phosphate. The reaction is driven energetically by proton-transport across the enzyme. The activity of the enzyme is regulated through a transmembrane pH-difference and a …
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Role of a-Subunit VISIT-DG Sequence Residues Ile-346 and Ile-348 in the Catalytic Sites of Escherichia Coli ATP Synthase.
<p>F<sub>1</sub>F<sub>O</sub>-ATP synthase is the primary source of cellular energy production in most living organisms. Malfunction of this enzyme is implicated in diseases. There are many functional motifs in and around the catalytic sites of this enzyme. One of them is the highly conserved …
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Molecular Modulation of a-Subunit VISIT-DG Sequence Residue Asp-350 in the Catalytic sites of <em>Escherichia coli</em> ATP Synthase.
<p>ATP Synthase is the fundamental means of cellular energy production in animals, plants, and almost all microorganisms. In order to understand the mechanism of ATP catalysis, critical amino acid residues involved in Pi binding have to be identified. The αVISIT-DG sequence at the interface of α/β …
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Requirement of ßDELSEED-Motif of <em>Escherichia coli</em> F<sub>1</sub>F<sub>O</sub> ATP Synthase in Antimicrobial Peptide Binding.
<p>F<sub>1</sub>F<sub>O</sub> ATP synthase is a membrane bound enzyme capable of synthesizing and hydrolyzing ATP. Lately, α-helical cationic peptides such as melittin and melittin related peptide (MRP) were shown to inhibit <em>E. coli</em> ATP synthase. The proposed but unconfirmed site of …
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Modulation of Alpha-Subunit VISIT-DG Sequence Residues Ser-347, Gly-351 and Thr-349 in the Catalytic Sites of <em>Escherichia coli</em> ATP Synthase.
… of inorganic phosphate (P<sub>i</sub>) in ATP synthase catalytic sites is a crucial step for the synthesis of adenosine-5'-triphosphate (ATP). ATP is the fundamental means of cellular energy in almost every organism, and in order to gain insight into the regulation of ATP catalysis, …
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Enforced Large-Scale Motions in Proteins
… and rotation of the central stalk within an ATP synthase F1 unit. SMD methods employed include linear constant-velocity extension, multiple-trial segmented path creation, and torque application to enforce angular velocities. The synthesis-direction rotation of ATP synthase central stalk led …
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