Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"ATP release"”.
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A novel approach to real-time monitoring of erythrocyte ATP release as a function of hypoxia
… in local vasodilatory signaling by releasing ATP in response to hypoxia. RBC ATP release and overall function are impaired with age and disease. Until now, luciferin/luciferase bioluminescence is the only method described to quantify ATP release from RBCs. Here, we describe a novel approach …
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Mechanisms of impaired red blood cell ATP release in older adults: implications for altered vascular control with age
… aim of determining the mechanisms of impaired ATP release from red blood cells (RBCs) of healthy older adults in response to hemoglobin deoxygenation and identifying a potential role of this impairment in the declines in vascular control of peripheral blood flow with advancing age. Advancing …
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Decoding Gating Properties of the Pannexin1 Channel: ATP Release and pH Sensitivity and the Potential Role in Health and Disease
… memory and sensory function. In sensory systems, ATP release and purinergic signaling is considered the most important function of Panx1. Here, the function in the olfactory epithelium was investigated using Panx1 knock out mice. Functional analysis in vivo confirmed a role in olfaction suggesting …
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ATP, TRP channels and airway neuronal sensitisation
… gaining a deeper understanding of the roles of ATP and transient receptor potential (TRP) channels in chronic cough by working with primary bronchial epithelial cells (PBECS) and an in-Vitro human sensory neuronal model differentiated from human dental pulp stem cells termed peripheral neuronal …
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ADP regulation of insulin secretion and beta-cell apoptosis
… muscle cells (VSMCs) through ectonucleotidase or ATP release under the effect of high glucose. The expression of the ADP receptors P2Y1 and P2Y13 were shown in both mouse pancreatic islets and isolated β-cells using real-time PCR quantification. Insulin and glucagon secretion were measured both in …
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1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine (Platelet Activating Factor): Interactions with Sheep Platelets
… characterized in terms of aggregation, serotonin release, ATP release and phosphatidic acid formation but also with a new and more satisfactory method based on platelet shape change. The development of the latter was based on microscopic and rheooptic observations but is also supported by …
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Mutations in a Mechanosensitive Channel Enable Intravascular Metaststic Cell Survival
… physically deformed within vessels. Enhanced ATP release from PANX1 channels, which are activated during membrane stretch, acts as a cell autonomous survival signal during lethal cellular deformation. Functional characterization of additional nonsense and missense PANX1 5 mutations detected in …
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Localised Dosing and Nanodetection Using a Novel Scanning Ion Conductance Microscope and Its Application to Alzheimer's Disease
… one for detection of the adenosine triphosphate (ATP) release from these pores. A variety of different ATP sensors were made. The best had a sensitivity of 10 micro molar and works as a hexokinase-cofunctioned electrolyte-gated organic field-effect-transistor. However no statistically significant …
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Nature of Large Vesicle Exocytosis in Pancreatic β-cells: Release of ATP and GABA
… channel in secreting cells. The presence of ATP sensitive channel on the cell membrane permitted the electrical detection of minute (amoles) quantities of ATP and thus created a possibility to measure ATP released from a single insulin granule. The release of peptides and low molecular weight …
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Mechanisms of Calcium Wave Initiation and Propagation in Astrocytes
… that Ca<sup>2+</sup>waves are mediated by ATP diffusion in the extracellular space. This confusion arose because Cx43 channels are most studied in the gap junction configuration and no role could easily be ascribed to an intercellular channel in an extracellular paracrine signaling system. …
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The Sandwich Synapse: Trans-glial Signaling Between Neuronal Somata in the Dorsal Root Ganglion
… The first, NS to glial cell, synapse involves ATP release from the neuron and purinergic activation of the glial cell. The second, glial cell to NS, synapse is glutamatergic. We find that inter-NS transmission at the DRG Sandwich Synapse involves Ca
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Connexin-32 and Connexin-43 Immunoreactivity in Rodent Taste Buds
<p>Studies indicate that ATP is one of the primary neurotransmitters in taste transduction. ATP release occurs from taste cells via specific hemichannels such as pannexin/connexin hemichannels (Huang et al., 2007; Romanov et al., 2007). We hypothesize that Type II (receptor) and possibly Type III …
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Defining the Role of Pannexin1 in Skeletal Muscle and Satellite Cell Function in vivo and ex vivo Using a Novel Conditional Knockout Mouse Model
… 1 (Panx1) channels, well known for their role in ATP release, play a key role in myogenesis. However, its role in muscle development remains to be elucidated. Using a novel skeletal-muscle-specific Panx1 knockout (Panx1^smKO) mouse model, this thesis highlights the influences Panx1 plays in …
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Liposomal delivery of PDE5 inhibitors and UT-15C to human erythrocytes
… studies have shown that the controlled release of adenosine triphosphate (ATP) from human erythrocytes is an important mechanism for the regulation of vascular caliber. However, erythrocytes from patients with pulmonary arterial hypertension (PAH) fail to release ATP in response to the …
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Cardioprotective treatment strategies
… (t-PA) and extracellular adenosine triphosphate (ATP) release contribute to myocardial injury. ATP is degraded into adenosine by the enzyme apyrase, and adenosine possesses cardioprotective properties. ADC-1004 is an antagonist of the receptor to the activated complement factor C5a. Hypothermia …
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Investigating the role of the CCL2/CCR2 axis in monocytes and its modulation by extracellular nucleotides
… CCR2 activation indirectly by intracellular Ca2+ release, cell migration, and cell adhesion to vascular endothelium. Techniques such as lentivirus-mediated gene-knockdown, RT-PCR, and HPLC complemented these studies. A pharmacological approach indicated a requirement of CCR2, Ca2+, Gαi/o-type …
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The effect of specific phospholipids that may have a possible role in propagating or inhibiting glioma
… of each phospholipid by in vitro studies. The ATP release by 132 1N1, 005-3 or U87-MG cell line treated with each soy derived phospholipid was measured after 48 hrs of incubation. On measurement using the ATP assay, the results obtained from 132 1N1 and GOS-3 cell lines showed significant (P < …
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Vascular breakdown in diabetic retinopathy: Role of connexin43 and possible interventions
… in terms of pro-inflammatory cytokine and ATP release as well as expression of inflammatory proteins, in particular to resolve the role of connexin43 hemichannels in the innate immune system inflammasome pathway. Building upon this work, a new model of DR was created by intravitreally …
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The Physiological Role of Serotonergic Transmission in Adult Rat Taste Buds
… TRCs and taste qualities affected by serotonin release, and (2) by examining the net outcome from this release at the gustatory neural level. Phenotyping the 5-HT1A-expressing cells through double label immunocytochemistry and single cell reverse transcriptase PCR revealed that 5-HT1A is …
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Coronary Reactive Hyperemia
… involving purinergic signalling. Purines such as ATP and ADP have recently been discovered to play a vital role in the regulation of vascular tone. We postulated that ADP could play a vital role in the mechanism of coronary post ischemic reactive hyperemia and that increased concentrations of …
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