Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 167 for “"ATP binding"”.
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tissue-specific role of ATP binding cassette transporter A1 in atherogenesis
ATP binding cassette transporter A1 (ABCA1) facilitates cellular free cholesterol and phospholipid efflux across the plasma membrane to combine with apolipoproteins, forming nascent high density lipoproteins (HDL), the precursor of mature plasma HDL. Mutations in ABCA1 cause Tangier disease, …
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Charakterisierung eines neuen ATP-binding-cassette Transporters aus der ABCA-Subfamilie
Im Rahmen dieser Arbeit wurde mit ABCA5 aus Gesamt-RNA der humanen Hepatomzell-Linie HepG2 ein neues Mitglied der ABCA-Subfamilie aus der Superfamilie der ABC-Proteine isoliert und charakterisiert. Das ABCA5-Gen umfasst einen offenen Leserahmen (OLR) von 4926 bp und kodiert für ein Protein der …
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Regulation and functional significance of ATP binding cassette transporters in human placenta
The aim of this project was to study ATP binding cassette (ABC) transporters in the human placenta, in particular their regulation and role in trophoblast differentiation and survival. The presence and localisation of four major placental drug transporters, multidrug resistance gene product 1 and 3 …
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Site-directed mutagenesis of the ATP-binding site in spinach rubisco activase
… A significant difference in the kinetics of the ATP response between the two cloned isoforms was observed. The 45 kD polypeptide exhibited sigmoidal kinetics for both rubisco activase and ATPase activities, while the activities of the 41 kD isoform appeared to follow standard Michaelis-Menten …
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Characterizing the role of ATP-binding cassette transporters in triterpenoid wax bloom production
… have yet to be fully elucidated. Numerous ATP-binding cassette (ABC) transporters have been characterized in model systems and crops, and some members of the G transporter subfamily are linked to the production of epicuticular wax blooms. Many studies utilizing model systems focus on …
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Gates and Latches: the mechanochemistry of ATP binding in kinesin 1 and Eg5
… 1. A gating mechanism is needed to coordinate ATP binding to the two catalytic domains within Kinesin 1 in order to prevent simultaneous binding, hydrolysis and detachment of both heads which could prematurely terminate cargo transport. The second system investigated involves cell division and …
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Novel Strategies towards the Inhibition of ATP-Binding Cassette (ABC) Transporters from Pathogenic Bacteria
Multidrug transporters in the ATP-Binding Cassette (ABC) superfamily play critical roles in pathogenic bacteria. These transport systems are particularly important in conferring antibiotic resistance on the cell by mediating the efflux of a wide range of structurally unrelated compounds and the …
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ROLE OF HEPATIC APOLIPOPROTEIN M AND ATP-BINDING CASSETTE TRANSPORTER A1 (ABCA1) IN LIPID METABOLISM
Apolipoprotein M (ApoM) and ABCA1 are both expressed in hepatocytes, but their impact on lipid metabolism remains poorly defined. ApoM binds to plasma HDL via its retained signal peptide and transports sphingosine 1–phosphate (S1P). We found that liver–specific apoM transgenic mice had …
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The Roles of ATP-Binding Cassette Transporters G5 and G8 in Liver X Receptor-Mediated Sterol Trafficking
… expression of two target genes of LXRalpha , the ATP-binding cassette (ABC) transporters Abcg5 and Abcg8, is required for the increase in sterol excretion, the decrease in fractional cholesterol absorption, and the increase in fecal neutral sterol excretion associated with LXR agonist treatment. …
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Characterizing the Interaction of the ATP Binding Cassette Transporters (G subfamily) with the Intracellular Protein Lipid Environment
… equation and is mediated by members of the ATP binding cassette (ABC) transporter superfamily. ATP-binding cassette (ABC) transporters represent a group of evolutionarily highly conserved cellular transmembrane proteins that mediate the ATP-dependent translocation of substrates across …
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Regulation of Rubisco in Arabidopsis Thaliana Rca(-) Complemented With Arabidopsis Rubisco Activase Engineered at an ATP Binding Site
… greater than 42kD wild type and Q111S at ADP/ATP ratios known to exist in planta. Q111D showed a Rubisco activation rate at night time ADP/ATP greater than the 42kD wild type rate at zero ADP/ATP suggesting an altered response. Measurements of K$\rm\sb{d}$ ADP and K$\rm\sb{d}$ ATP with a …
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Investigations into the Lipid Transport Mechanisms and Energetics of Bacterial ATP-Binding Cassette (ABC) Transporters MsbA and LmrA
… MsbA are two paradigms in research on multidrug ATP-binding cassette (ABC) transporters. The bacterial transmembrane protein LmrA from *Lactococcus lactis* was the first known prokaryotic ABC transporter homologue of the mammalian multidrug resistance transporter ABCB1 (P-glycoprotein) …
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THE ROLE OF HEPATOCYTE ATP-BINDING CASSETTE TRANSPORTER A1 (ABCA1) AND APOLIPOPROTEIN M (APOM) IN HIGH DENSITY LIPOPROTEIN (HDL) METABOLISM
Hepatic ATP binding cassette transporter A1 (ABCA1) and apolipoprotein M (apoM) both have been shown to influence high density lipoprotein (HDL) metabolism. Hepatic ABCA1, a plasma membrane protein, functions to transport free cholesterol (FC) and phospholipids across the plasma membrane to …
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An investigation on role of the ATP-binding cassette B5 (ABCB5) transporter as potential mediator of melanoma resistance to BRAF inhibition
… in the review in Chapter 1. Overexpression of ATP-binding cassette (ABC) transporters has been linked to multidrug resistance in numerous cancer types. These transporters expel the anti-cancer drugs out of the cell, thereby decreasing the intracellular concentration of the drug. In melanoma, …
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Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases
… et al., 1983) is based on the demonstrated binding of insulin to intracellular membrane structures (see chapter 2, section 2.2.1), and the in vitro effect of insulin on nuclear envelope phosphorylation, NTPase activity, and mRNA efflux (see chapter 5, section 5.1). These independent …
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Mutational analysis of the tobacco etch potyvirus cylindrical inclusion protein
… 15b constructs were expressed and assayed for ATPase, ATP binding (only those proteins that were ATPase activity deficient) and RNA binding activities. The pET 11a constructs were expressed and assayed for ATP binding (only those proteins that were ATPase activity deficient) and RNA binding …
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The Molecular Mechanism of the Escherichia Coli vitamin B12 Transporter BtuCD-F: Real-time Observation of the Transporter in Motion
… are open to cytoplasm in the resting state, and ATP binding induces a major conformational change resulting in opening of the TMDs instead to the periplasm. However, the crystal structures of BtuCD from E. coli and recent EPR spectroscopy studies indicate that this type II importer employs a …
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Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine
ATP-powered proteases enforce protein quality-control and regulation in all domains of life. ClpX, a AAA+ ring homohexamer, uses the energy of ATP binding and hydrolysis to power conformational changes that unfold and translocate target proteins into the ClpP peptidase for degradation. X-ray …
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Mechanistic studies of ABC transporters
ATP-binding cassette (ABC) transporters are a superfamily of primary membrane transporters that are abundant yet highly diverse in structures and functions. These proteins power the active transport of a large variety of substrates across biological membranes, by harnessing the energy of ATP …
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