Global ETD Search
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Showing 1 to 14 of 14 for “"ATG16L1"”.
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Novel mechanisms of Atg16L1 recruitment in non-canonical autophagy
… lipidation to lysosomes. This project focuses on Atg16L1, an essential autophagy protein that directs the membrane site where LC3 is lipidated. Atg16L1 is relatively well characterised in autophagy but little is known about the mechanisms underlying its role in non-canonical autophagy. This …
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Novel Functions of ATG16L1 in the Innate Response to Invasive Bacterial Pathogens
… has been implicated in both of these functions. ATG16L1 is a central component of the autophagy machinery and is a CD susceptibility locus. Here, two novel functions of ATG16L1 in the innate immune response are described. First, we uncovered a regulatory role for ATG16L1 in the inflammatory …
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Characterisation of a novel interaction partner for Atg16L1 and its role in regulating autophagy
… Of particular importance is Atg16-like-1 (Atg16L1), which through its interactions with Atg5-Atg12, specifies the site of LC3 incorporation into the expanding autophagosomal membrane. In this study tools have been generated for the study and characterisation of Atg16L1 domains in cell …
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Structural and functional characterization of the autophagy proteins Atg5 and Atg16L1 and their interaction partners
… wurde das Oligomerisierungsverhalten von Atg5~Atg16L1 Komplexen mit unterschiedlich langen Atg16 Konstrukten untersucht und mittels Multiwinkel-Laserlichtstreuung analysiert. Die Coiled-Coil Domäne von Atg16L1 dimerisiert, so dass jeweils zwei Kopien von Atg5 und Atg16L1 im Atg5~Atg16L1 Komplex …
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Use of transgenic mice to study the role of autophagy protein ATG16L1 in Crohn’s Disease
… (SNP) conferring T300A in the autophagy gene ATG16L1 as a CD susceptibility locus1. ATG16L1 is an essential component of the autophagy pathway, a conserved cellular degradation pathway also linked to bacterial defence2. It is not known how disruptions in ATG16L1 or autophagy contribute to CD …
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Use of an Atg16l1 mouse model to study the role played by autophagy in neuroprotection
Atg16l1 is an autophagy protein, most known for its association with Crohn’s disease. While knockout models of autophagy pathway components recapitulated neurodegeneration in laboratory mouse, the effect of Atg16l1 KO on neuronal protein homeostasis has been unknown. This study found Atg16l1 KO …
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Polar Body Membrane Breakdown Occurs Independent of Macroautophagy in C. Elegans
… polar body during embryonic development. ATG16L1 has separable roles in regulating LC3 recruitment during macroautophagy and LAP. We demonstrated that the ATG16L1 <em>C. elegans</em> orthologs, ATG-16.1 and ATG-16.2, function redundantly to promote polar body membrane breakdown. We also …
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Neuroprotective Effects of Sulforaphane and the Involvement of Autophagy
… cells (PNCs) were developed from embryos from Atg16L1 wild type and knock out (KO) mice. Atg16L1 is a protein necessary for autophagosome formation. Immunostainings assessed that autophagy was indeed fully supressed in KO cells. Preliminary results suggest that autophagy is involved in the …
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Identification of novel genetic determinants in the high prevalence early-onset inflammatory bowel disease population in Scotland
… susceptibility genes (NOD1/CARD4, IL23R, ATG16L1, IRGM, FLG) was performed to enable single variant and haplotype-tagging association studies. Genotypic data of population-matched healthy controls were obtained locally (n=342) and from the Wellcome Trust Case Control Consortium (n=2937). …
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Mechanism and Functions of STING-Induced Noncanonical Autophagy
… the interaction between V-ATPase and the ATG16L1-WD40 domain on Rab7-coated endolysosomal compartments. Additionally, we unveiled that STING-induced noncanonical autophagy leads to the activation of MiT/TFE transcription factors (TFEB, TFE3, and MITF), promoting endolysosomal biogenesis. …
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Visualization and quantification of autophagy in primary gastrointestinal epithelia cell cultures.
… mutations in the autophagy related (Atg) protein Atg16L1, and NOD2 as risk alleles for Crohn’s disease. Atg16L1 is essential for the formation of autophagosomes and autophagosome formation is stimulated following recognition of bacterial cell wall peptide muramyl dipeptide (MDP) by NOD2. Crohn’s …
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Interplay between Cannabinoids, SOCS3, and Autophagy Process in the Intestinal Epithelium with the Use of an Established in vitro Model System.
… associates Crohn's Disease (CD) with an impaired ATG16L1 autophagy gene. Increased activity in the endocannabinoid system and up-regulation of suppressor of cytokine signalling (SOCS)-3 protein expression are evident in inflamed intestine. We accessed the impact of phyto-cannabinoid (CBD), …
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Biochemical and Structural Characterization of the Atg8/LC3 Lipidation Pathway
… LC3 or GABARAP, ATG7, ATG3, and the ATG12–ATG5-ATG16L1 complex for in vitro studies of LC3/GABARAP lipidation; based on these protocol established, we report that an Atg3 element we term E123IR (E1, E2, and E3-interacting region) is an allosteric switch, by studying yeast enzymes. Nuclear …
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The role of CASM in the endolysosomal damage response
… lipidation occurs via the central V-ATPase – ATG16L1 axis, and onto phosphatidylserine (PS), a highly specific hallmark of CASM. This was in stark contrast to the previous consensus, where all ATG8 lipidation observed following damage was assumed to be present on autophagosomes facilitating …