Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"AT-rich"”.
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Human common fragile site FRA16D flexibility peak is not a strong mitotic: Recombination hotspot in <i>saccharomyces cerevisiae</i>
… fragile sites (CFS) are areas of the genome that tend to break when DNA replication is stressed or partially inhibited. Breaks at CFS can lead to gene deletions and amplifications that can result in the genesis of cancer cells. There is controversy about the mechanism of CFS instability. This …
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Human common fragile sites, identification, and analysis of breaks using <i>saccharomyces cerevisiae</i>
… areas of the genome prone to breaks under replication stress, are frequently altered in tumor cells. Two hypotheses have been proposed to explain why common fragile sites break: (1)AT-rich segments with high flexibility in the fragile site fold into secondary structures, leading to replication …
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Control of uteroglobin gene expression
Eukaryotic nuclear genes are regulated by a complex array of transcription factors that influence RNA polymerase II activity. Included among these factors are the cellular receptors for steroid hormones. The rabbit uteroglobin gene expression is positively stimulated by progesterone, and binding …
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Sequence characterization of the HSA12/22/12 evolutionary breakpoint region on SSC5q21
… characterized. The existence and location of the breakpoint regions were originally determined in the human-porcine comparative map constructed by Meyers and colleagues. Bacterial artificial chromosome (BAC) fingerprint analysis and radiation hybrid mapping of BAC-end sequences was …
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Physical mapping of an early sea urchin gene battery from Parenchinus angulosus
… was to characterise an early histone gene battery isolated from Parenchinus angulosus. An early histone gene battery (named H27) which was believed to have been isolated from Parenchinus angulosus, appeared by restriction enzyme mapping and partial sequencing to be identical to H22, an early …
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Footprint Analysis of the Transcriptional Control of Glycogen Phosphorylase 2 in Dictyostelium Discoideum
… breaks down glycogen into glucose monomers that are subsequently used to synthesize the terminal end products of cellular differentiation. This gene is an ideal candidate for studying the process of selective gene expression because its product figures so prominently in the development of this …
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H-NS Counter-silencing as a Primary Regulatory Mechanism of Curli Gene Expression in Salmonella Typhimurium
Incorporation of horizontally acquired genes into transcriptional networks is essential for regulated expression of virulence in the bacterial pathogen Salmonella enterica serovar Typhimurium. The nucleoid-associated protein H-NS is able to recognize and bind AT-rich horizontally acquired genes, …
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PCR-based Synthesis of Codon Optimized cry2Aa Gene for Production of Shoot and Fruit Borer (Leucinodes orbonalis) Resistant Eggplant (Solanum melongena L.) Cultivars
… is a major limiting factor in commercial cultivation of eggplant in southeast Asia. Extensive use of pesticides as well as the conventional breeding methods have been ineffective in controlling the borer so there is a need for Integrated Pest Management (IPM) strategies for its control. Bacillus …
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Towards the development of an RT-PCR protocol for malaria parasite genes
Malaria is a life-threatening parasitic disease, which is becoming increasingly problematic in Sub-Saharan Africa due to the spread of drug-resistant parasite strains. Novel therapeutic targets and drugs are thus required to successfully treat and/or prevent malaria. The aim of this study was the …
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Interactions between mobile genetic elements and their bacterial hosts
Integrative and conjugative elements (ICEs) are widespread mobile genetic elements that facilitate the spread of many important genes, including those involved in antibiotic resistance, metabolism, pathogenesis, or symbiosis. These elements are also powerful tools for genetic analyses and …
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New mechanisms in transcription regulation and chromosome organization in Caulobacter crescentus
… to environmental cues or growth conditions. Activation or repression of transcription is often carried out by proteins, called transcription factors, that interact with DNA or RNA polymerase (RNAP) or both, and can change the preference of RNAP for target promoters. Additionally, DNA is tightly …
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The NADP-dependent glutamate dehydrogenase of Giardia lamblia: a study of function, gene structure, and expression
… important protozoan parasite, but its location in the phylogenetic tree is at a critical and informative position. Characterization of its subcellular structure and rRNA sequences suggest that Giardia is one the most primitive eukaryotes known. Giardia has an anaerobic metabolism that uses …
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Genome engineering and functional gene regulation tools for the study of malaria parasites
Plasmodium falciparum is the causative agent of the most severe form of human malaria, a mosquito-borne disease that remains a major global health problem. The efforts to create new antimalarial drugs and effective vaccines have been significantly hindered by the lack of robust tools for performing …
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Gene Flow and Dispersal Among Populations of the Stonefly <i>Peltoperla tarteri</i> (Plecoptera: Peltoperlidae) in the Southern Appalachians
… number of recent studies focused on the issue, patterns of stream insect dispersal in temperate streams of North America remain poorly understood. Movement of benthic invertebrates is notoriously difficult to measure directly using traditional means; however, genetic markers provide an ideal …
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Discovery of a Novel Metalloregular Family: Structure/Function Studies of E. Coli Rcnr
… diverse processes including photosynthesis, oxidative respiration and protein translation. Despite these essential functions, excess metal ions can lead to cell death through the generation of oxidative damage or the occupation of non-native metal sites. Acquiring metal ions from the environment …
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Targeting Lsr2/DNA Complexation for Dysregulation of Gene Expression in <i>Mycobacterium tuberculosis</i>
The identification of new drug targets and mechanisms of action against tuberculosis has become urgent, as the emergence of drug-resistant strains has made many treatments ineffective. Lsr2 is a protein encoded by the <i>Mycobacterium tuberculosis</i> genome that acts as a global repressor of …
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CO2 absorption into concentrated carbonate solutions with promoters at elevated temperatures
As atmospheric carbon dioxide (CO2) emissions continue to increase, it becomes important to develop methods to capture CO2 from large emission sources such as power plants. The Hot Carbonate Absorption Process with Crystallization Enabled High Pressure Stripping (Hot-CAP), which employs a …
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Controls Over S-Phase And Over Nuclear Synchrony
… Schizosaccharomyces pombe, we have investigated the controls over S-phase within the cell cycle and over S-phase and mitosis among multiple nuclei within a common cytoplasm. To achieve faithful replication of the genome in each cell cycle, re-initiation of S-phase is prevented in G2 and …
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Transcriptional Regulation of the Glycogen Phosphorylase-2 Gene in <I>Dictyostelium discoideum</i>
… phosphorylase- 2</I> gene (<I>gp2</I>) is initiated during early development and regulated by the extracellular morphogens cAMP and Differentiation Inducing Factor (DIF-1) [1-3]. Glycogen phosphorylase- 2 catalyzes the breakdown of glycogen reserves in developing cells to generate glucose …
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Centromere identification and inactivation on a neo-Y chromosome fusion in threespine stickleback fish (Gasterosteus aculeatus)
… is essential for proper chromosome segregation during both mitosis and meiosis. Chromosomes containing two centromeres (dicentric) often mis-segregate during cell division, resulting in aneuploidy or chromosome breakage. Dicentric chromosomes can be stabilized by centromere inactivation, a …
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