Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"ASF1"”.
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Investigation of The Roles of Asf1 and Caf-1-Mediated Chromatin Assembly In The Human Dna Damage Response
… via knockdown of the histone chaperones ASF1A, CAF-1 or a mutation that specifically prevents ASF1 binding to histones, hinders loading of Rad51 onto ssDNA during homologous recombination, as a consequence of reduced recruitment of the Rad51 loader MMS22L/TONSL to ssDNA, resulting in …
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The structural analysis of histone H3 lysine 56 acetylation and related histone chaperone complexes
… dimers are supplied by Anti-Silencing Factor 1 (Asf1).<br/><br/>In yeast, newly synthesised H3 is acetylated at multiple sites in complex with H4 by the histone acetyl transferase (HAT) Regulator of Ty1 Transposition (Rtt109). The activity of Rtt109 towards H3 tail residues and H3 lysine 56 (K56) …
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Characterisation of the (H3-H4)2-tetramer and its interaction with histone chaperones
… when bound to the histone chaperone Asf1 the H3-H3’ interaction is disrupted, thus Asf1 effectively splits the tetramer binding a single H3-H4 dimer. Using targeted protein crosslinking and pulsed EPR we determine that, unlike Asf1, the Nap1 family of histone chaperones can bind H3-H4 …
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Isolation and Characterization of Proteins Interacting with Tobacco Transcription Factor TGA2.2
… haben hergestellt, dass as-1 Element vom ASF1 / SARP Proteinkomplexes aus Nicotiana tabacum erkannt werden. Als Komponenten des ASF1 / SARP, konnten die Klasse II TGA Faktoren, TGA2.2 und TGA2.1, identifziert werden. Zur identifizierung potentieller Interaktionspatner von TGA2.2 wurde im …
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A Two-colour Reporter Screen and Application to Cell Cycle Transcription
… Rtt106 works with two other chaperones, Asf1 and the HIR complex, to create a repressive chromatin structure at histone promoters which is bound by the protein Yta7. It was clear from previous work that Asf1 and HIR repress transcription at HTA1 and that HIR localizes to and functions …
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Molecular and Genetic Analysis of the Role of Ku in Silencing and Telomere Function in Saccharomyces Cerevisiae
… in silencing (YKU70, SIR1, SAS2, SAS5, SET1, ASF1) and telomere length regulation (YKU70, BDF1, ANC1, RAD50, TEL1, RAD52). Double mutant analysis revealed functional relationships between genes by generating a network of 241 synthetic interactions between 144 genes at 30°C and a network of …
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Identification of MMS22 as a regulator of DNA repair
… with NF?BIL2/TONSL, the histone chaperone ASF1 and subunits of the MCM replicative helicase. MMS22L colocalizes with TONSL at perturbed replication forks and at sites of DNA damage. MMS22L and TONSL are important for the repair of collapsed replication forks as depletion of MMS22L or TONSL …
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Role of Nuclear Hat1p Complex and Acetylation of Newly Synthesized Histone H4 in Chromatin Assembly
… that is similar to that observed in an asf1¿¿¿. Lastly, the role of Hat1p and Hif1p is at least partially redundant with the role of the histone chaperone Asf1p in the DNA repair linked chromatin reassembly process. The acetylation on lysine 5 and 12 of newly synthesized histone H4 is …