Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"ARF6"”.
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The involvement of ARF6 in rapid membrane recycling during Drosophila spermatocyte cytokinesis
… trafficking during cleavage furrow ingression. ARF6 is a member of the ARF family of small GTPases, and previous studies suggest that it is an important regulator of membrane trafficking through the endocytic pathway, and cortical Actin remodelling. I generated an arf6 null mutant in Drosophila. …
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Endosomal membrane dynamics underlying cell spreading: A role for the small GTPase Arf6
… role of a specific member of the Arf subfamily Arf6, in this process. In contrast to other studies which have primarily used constitutively active or dominant negative mutants of Arf6 to study its cellular function, we employed mouse genetics. In this system, mouse embryonic fibroblasts (MEFs) …
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An Investigation into the Role of Arf6 in MCH-mediated Rearrangements in 3T3-L1 Preadipocytes
… activation of ADP-ribosylation factor 6 (Arf6). These events in succession may result in destabilized actin in murine 3T3-L1 pre-adipocytes. A pharmaceutical inhibitor of Arf6, NAV-2729, as well as the overexpression of dominant negative ARNO/Arf6 plasmids were used to determine if Arf6 …
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Small gtp-binding proteins in insulin secretion
… exchange factor [GEF] for the small G-protein Arf6, mediates the activation of Arf6, and that ARNO/Arf6 signaling axis, in turn, controls the activation of downstream effectors. Salient features of my study are: [i] ARNO/Arf6 is expressed in clonal β-cells, rodent islets and human islets; [ii] …
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EFA6/PSD3, Arf6 Modulator, Regulates Ethanol-Induced Behaviors Via the Insulin Receptor Signaling Pathway in Flies and Humans
… a guanine nucleotide exchange factor (GEF) of Arf6, EFA6, molecularly regulates the activity of Arf6 as well as the behavioral output of Arf6. I show that EFA6 mutant flies are 1) hypersensitive to the effects of ethanol 2) they do not develop tolerance following a pre-exposure, and 3) altered …
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Charakterisierung der peroxisomalen ARF-Bindung
… Säugern bekannten ARF-Isoformen konnten ARF1 und ARF6 an der Peroxisomenmembran identifiziert werden. Funktionelle Studien zeigten, daß ARF1 nicht jedoch ARF6 für die Rekrutierung von Coatomer an Peroxisomen essentiell ist. Dabei modulieren ATP und Faktoren aus dem Cytosol in synergistischer Weise …
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Endocytosis mechanism of poly- and lipoplexes in siRNA delivery in HeLa cells
… enter through caveolin-, flotillin- and ARF6-mediated endocytosis pathways. ARF6 might play multiple roles in the delivery and release of the delivered siRNA in cells. Lipoplexes, on the other hand, enter the cells and release siRNA through a method of direct fusion with the plasma …
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Signalbindung und Membraninteraktion von heterotetrameren Adaptorprotein-Komplexen
… simuliert wurde. Unsere Daten zeigen eine von ARF6-unabhängige, aber von Phosphatidylinositolen-abhängige Rekrutierung von AP2. Durch den Vergleich von phosphoryliertem und nicht-phosphoryliertem AP2 konnte bestätigt werden, dass die Interaktion mit Tyrosin-haltigen Signalen durch die …
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Novel interactions of the 5-HT₂ₐ and related receptors with intracellular signalling proteins
… receptor associates with both ARF1 and ARF6, as shown by in vitro GST interaction assays.
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The study of two transmembrane autophagy proteins and the autophagy receptor, p62
… proteins traffic via the dynamin-independent ARF6-associated pathway. Moreover, I also show that these proteins physically interact with each other. In addition, the tools developed during these studies helped me identify a new role for the most common autophagy receptor protein, p62. I show …
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Heparan Sulfate Proteoglycans in Pancreatic Ductal Adenocarcinoma (PDAC)
… Specifically, YAP1 transcriptionally regulate ARF6 GAPs in an indirect manner to promote SDC1 localization on the cell surface and sustain macropinocytosis, enhance the activation of multiple RTKs, thereby promoting resistance to KRAS*-targeted therapy. Overall, our study has provided the …
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Studies of E2s Related to Unconventional Ubiquitination in Arabidopsis thaliana
… ARFs were evaluated, and overexpression of ARF6 enhanced the root hair growth in WT plants and partially rescued the root phenotypes of the ubc13 mutant. A major part of this study was on Arabidopsis UBC22, a sole member of an Arabidopsis E2 subfamily. Results from the phylogenetic analysis …
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Deciphering ARAP3 Functions in Hematopoiesis and Hematopoietic Stem Cells
… is a GTPase-activating protein that inactivates Arf6 and RhoA GTPases. ARAP3 deficiency in mice causes a sprouting angiogenic defect resulting in embryonic lethality by E11. Mice with an ARAP3 R302,303A mutation (KI/KI) that prevents activation by PI3K have a similar angiogenic phenotype, …
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Characterizing the Expression Pattern of miR167-Regulated ZmArf3 and ZmArf30 in Maize Inflorescences
… to the functionally redundant Arabidopsis ARF6 and ARF8, which are also targeted by miRNA167. ZmArf3 and ZmArf30 have similar expression patterns in the maize tassel, localizing in all meristem regions, including the inflorescence meristem, branch meristem, spikelet pair meristem, spikelet …
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Trafficking motifs in potassium channels
… by a dynamin-independent, raft-mediated, and ARF6-dependent pathway. A prolonged block of this pathway revealed that the channels could also undergo internalisation by an alternate dynamin-mediated pathway. Internalised hERG channels were found to recycle back to the cell surface and undergo …
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Investigation of the intracellular trafficking pathways of the dermonecrotic toxin family
… study implicates the small regulatory GTPase Arf6 in the endocytic trafficking of PMT. Translocation of PMT from the endocytic vesicle occurs through a pH-dependent process that is also dependent on both microtubule and actin dynamics, as evidenced by inhibition of PMT activity in our …