Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"APOPLAST"”.
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Engineering and characterization of single chain antibody fragments (scFvs) specific to key enzymes in polyamine biosynthesis and manipulation of polyamine pathway by constitutive expression of recombinant ODC and SDE enzymes in transgenic tobacco
… vector for transient expression in the apoplast, cytosol and endoplasmatic reticulum. ODC was accumulated to higher levels in plant cytosol and apoplast, while ODC targeted to ER was not detected in immunoblots. Stable transformed tobacco plants overexpressing heterolougs ODC in cytosol …
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Investigating variation in the response of photosynthetic traits to ozone pollution in maize
… reacts to form reactive oxygen species in the apoplast, and ultimately decreases photosynthetic carbon gain. Free Air Concentration Enrichment (FACE) technology is for essential evaluating the photosynthetic response of maize to elevated ozone in field conditions. This project used FACE …
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Molecular characterisation of the <i>Rhynchosporium commune</i> interaction with barley
… using a proteomic analysis of infected plant apoplast.<br/><br/>Further apoplastic analysis revealed some of the most abundant proteins that are present in R. commune’s infection toolkit. Cell wall degrading enzymes (CWDEs), virulence factors and proteins involved in detoxification were all …
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Role of the Leucine-responsive Regulatory Protein during growth of the bacterial corn pathogen Pantoea stewartii subspecies stewartii in the xylem environment
… stage of Pss infection begins in the mesophyll apoplast of the corn leaf where a type III secretion system (T3SS) and its associated effectors induce water soaking (WS) and nutrient release. Ultimately, Pss will enter the plant xylem apoplast (will be referred to as the xylem) and use quorum …
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Production of recombinant human serum albumin in transgenic plants and plant cells
… when the protein was either secreted to the apoplast or targeted to endoplasmic reticulum (ER). Further analysis included stable transformation of tobacco plants via Agrobacterium mediated gene transfer. Accumulation of rHSA in the ER and secretion into the apoplast were confirmed by …
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Blueberry Fruit Development and Splitting
… microscope level that allowed us to analyze apoplast to symplast ratios (A:S ratio) and thus allowed a study of anatomical features that should be strongly influenced by physiological/hydrostatic phenomena. Several hypotheses of A:S ratios were tested resulting in the observed correlations …
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The role of citrate in plant-pathogen interactions
… acid citrate, which is secreted into the apoplast by both bacterial pathogens and plant hosts has previously been shown to increase the virulence of the gram negative pathogen Pseudomonas syringae DC3000 (Pst DC3000), by acting both as a chemoattractant and as an inducer of genes …
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Responses of wheat to infection by Mycosphaerella graminicola
… wheat infection, especially visualisation in the apoplast. None of the stains tested were suitable for this purpose, implying that the fungal hyphal wall is modified or protected within the plant
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Comparative study of the subcellular localisation and the N-glycosylation patterns of a recombinant glycoprotein expressed in Oryza sativa, Nicotina tabacum and Medicago truncatula
… secreted, via the default pathway, to the apoplast. This has been well-documented in protoplasts and leaf cells, but the trafficking of recombinant proteins in storage tissues such as endosperm or cotyledons has only received little attention. In addition, it has been suggested that the …
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The relevance of adenylate levels and adenylate converting enzymes on metabolism and development of potato (Solanum tuberosum L.) tubers
… similar signaling mechanisms in the plants’ apoplast. Presumably, apoplastic apyrases are involved in this signaling by hydrolyzing the signal mediating molecules ATP and ADP to AMP. This PhD thesis focused on the role of adenylates on metabolism and development of potato (Solanum tuberosum) …
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IDENTIFICATION OF THE ROLE OF [METHYL]GLUCURONIC ACID ON ARABINOGALACTAN POLYSACCHARIDES IN ARABIDOPSIS THALIANA
… a GPI-anchor. They can also be soluble in the apoplast. Their low abundance, chemical similarity and high functional redundancy have hindered their study. My strategy to overcome these difficulties was to study knock-out Arabidopsis thaliana plants of glycosyltransferases that transfer sugars …
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Characterization of ammoniumtransporters in Arabidopsis thaliana
… including the cytosol, the vacuole and in the apoplast (Wells and Miller, 2000; Nielsen and Schjoerring, 1998). Two related families of ammonium transporters (AMT1 and AMT2), containing six genes which encode transporter proteins that are specific for ammonium had been identified prior to this …
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Iron and reactive oxygen in wheat-pathogen interactions
… I show that iron is secreted to the apoplast of the diploid wheat Triticum monococcum during attack by the powdery mildew fungus Blumeria graminis f.sp. tritici. This iron accumulates at cell wall appositions synthesised de novo beneath sites of pathogen attack. I further show, using …
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The role of the apoplastic transport barriers for radial water and ion uptake in rice (Oryza sativa L.) and corn (Zea mays L.) roots
… LpOPR could be either brought about by a large apoplastic component of water transport or by a high permeability of membranes of the living cells in the OPR or by both together. In order to quantify the relative contribution of the apoplastic vs cell-to-cell paths to the overall LpOPR, …
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Transport of oxygen in roots of rice (Oryza sativa L.) and of water in developing grape berries (Vitis vinifera L.)
… grown plants correlated with development of the apoplastic barriers in the root peripheral layers. Deoxygenated conditions induced early development of exodermal Casparian bands and suberin lamellae. In addition to suberization, early lignification of walls of densely packed sclerenchyma cells …
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Engineering Resistance to <i>Orobanche</i> <i>aegyptiaca</i>: Evidence of Sarcotoxin IA as an Anti-Parasite Protein and Macromolecule Movement From Host to Parasite
… plants. These parasites form symplastic and apoplastic connections with their hosts and act as strong sinks for the uptake of water, minerals, and photosynthates, often causing severe damage to the hosts. Although the uptake of small molecules such as sugars and herbicides by <i>Orobanche</i> …
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Einsatz von Pflanzenzellkulturen als industriell nutzbare Expressionssysteme für therapeutische Proteine
… ensured the secretion of the protein to the apoplast. Main focus of the described studies are such parameters, which are of essential importance for the industry, like productivity, genetic stability of the organism as well as the production of a functional protein and the economy of the …
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In planta studies of the corn pathogen Pantoea stewartii subsp. stewartii and applications of a corn-based industrial byproduct
… Chaetocnema pulicaria. After entering the apoplast of the leaf, the bacteria cause water soaking symptoms before traveling to the plant xylem to form a dense biofilm, thereby blocking water transport and inducing necrosis and wilt. This results in reduced crop yield and may even lead to …
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