Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"APEX2"”.
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Directed evolution of split APEX peroxidase
… this thesis describes the development of a split APEX2 system. Short enzymatic reconstitution times are also desired, to further ensures both organelles' morphological integrity. Thus, it is critical that split APEX2 reconstitute peroxidase activity both rapidly and robustly. We first performed …
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Investigating how mitophagy is regulated by the metabolic context
… I secondly developed a proteomic approach using APEX2 biotin proximity labelling to detect changes in mitophagy signalling from the mitochondria surface. I exploited the in vitro and in vivo validated mito-QC reporter to target APEX2 on the mitochondrial surface using a conditionally stable …
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O-GlcNAc-Mediated Protein-Protein Interactions in Cell Signaling
… manner. We fused OGT to the peroxidase APEX2 and expressed it in cultured human cells. When cells are treated with cell-permeable biotin-phenol and H2O2, APEX2 generates biotin-phenoxyl radicals in situ, which react rapidly with proteins or other biomolecules, covalently attaching biotin …
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Directed evolution of TurboID for efficient proximity labeling in living cells and organisms
… Part I, we describe how fusion of the PL enzyme APEX2 to various mitochondrial proteins could be used to map the proteomes of mitochondrial subdomains and be used to visualize the localization of mitochondrial proteins in mitochondrial subdomains using APEX2 to generate contrast for electron …
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Enzyme engineering by directed evolution and applications in cellular imaging and proteomic mapping
… and characterization of the highly-active APEX2 which enabled advancements in mitochondrial and mitochondria-endoplasmic reticulum junction biology.
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Engineered heme peroxidases as genetically encoded probes with diverse applications in cell biology
… discuss the discovery and characterization of APEX2, a greatly improved variant of APEX. Finally, we describe the development of a split HRP protein complementation assay using a combination of rational design and directed evolution, followed by our application of split HRP for detection of …