Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"APE1"”.
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Analysis of nuclear transport signals of the human apurinic/apurimidinic endonuclease (APE1/REF1)
… in human apurinic/apyrimidinic endonuclease (APE1), a key protein in both DNA base excision repair and transcriptional regulation, has not been analyzed in detail. We examined the role of specific residues in nuclear translocation of APE1, using green fluorescent protein (EGFP) fused to APE1 …
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Targeted Inactivation of APE1 Endonuclease by E96A Mutation Sensitizes Pancreatic Ductal Adenocarcinoma to DNA-Damagin Therapy
… survival. Apurinic/apyrimidinic endonuclease 1 (APE1/Ref-1) is a multifunctional protein with critical roles in base excision repair (BER) and redox regulation of transcription factors. This dissertation tests the hypothesis that selective disruption of APE1 endonuclease activity destabilizes …
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Charakterisierung der Eaf 1-Funktion für die Biogenese der Aminopeptidase 1
… wird die Leucin-Exopeptidase Aminopeptidase 1 (Ape1) als Vorläuferform im Zytoplasma synthetisiert, zu Homododecameren oligomerisiert, anschließend über den Cvt-Transportweg in die Vakuole transportiert und dort prozessiert. Die Isolierung und Charakterisierung zweier Mutanten via10 und via14 …
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The Choreography of Base Excision Repair
… human apurinic/apyrimidinic endonuclease 1 (APE1) and DNA polymerase beta (Pol β). First, we report seven X-ray crystallographic structures that reveal how APE1 influences the conformations of a variety of different mismatched base pairs. We show that the conformation of DNA mismatches bound …
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DNA Repair, Redox Regulation and Modulation of Estrogen Receptor Alpha Mediated Transcription
… endonuclease 1 or redox factor-1 (Ape1/Ref-1), interacts with ERalpha, promotes the ERalpha-ERE interaction, influences ERalpha-mediated transactivation, and selectively associates with endogenous, estrogen-responsive genes in MCF-7 cells. Our findings suggest that NM23-H1 and …
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the impact of Ape/Ref1 on hypoxia regulated genes; potential applications for cancer
… AP endonuclease1/Redox effector factor 1 (APE1/Ref-1) is upregulated in human pancreatic cancer cells and modulation of its redox activity blocks the proliferation and migration of pancreatic cancer cells. Modulation of APE1/Ref-1 using the siRNA increases the HIF target, HMOX1 and NQO1,two …
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Cross-talk-free dual-color fluorescence cross-correlation spectroscopy for high-throughtput screening
… color channels. The enzymatic activity of APE1 was monitored by FCCS using a double-stranded DNA substrate that was dual labeled with Cy3 and IRD800. Activity of APE1 was also monitored in the presence of an inhibitor (7-nitroindole-2-carboxylic acid). To improve sample processing …
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Functional DNA repair capacity assays : a focus on base excision repair
… and their immediate downstream endonuclease (APE1) can be tested simultaneously, at single-cell resolution, under physiological conditions. Taking advantage of the transcriptional properties of several DNA lesions we have designed and engineered specific fluorescent reporter plasmids for OGG1, …
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The Development of Novel Apurinic/Aprymidinic Endonuclease/Redox-factor 1 Inhibitors for the Treatment of Human Melanoma
… DNA repair endonuclease-1 (APE1), first recognized as an important DNA excision repair enzyme, is also known as Redox Factor-1 (Ref-1) involved in the activation of many nuclear transcription factors in both redox-dependent and independent manner. It has been well-documented …
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High-resolution mapping of abasic sites and pyrimidine modifications in DNA
… following depletion of the key repair enzyme, APE1, where an increase was observed in the number of enriched loci compared to control cells. A relationship was also revealed between this form of DNA damage and coding and regulatory regions of the genome upon knockdown of the APE1 protein. The …
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Clarifying the Role of the CST Complex in DNA Replication and Repair
… involved in the BER pathway including Pol β, APE1, FEN1, and LIGI. CST’s ability to augment these BER-associated proteins could be instrumental in promoting efficient DNA repair. Additionally, we find that CTC1 and STN1 are able to significantly enhance the polymerase activity of Pol δ and Pol …
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Essentiality of Mitochondrial Genome Integrity in Cell Survival
… and 3’ blocking group by DNA glycosylase and APE1, respectively. Currently, three 5’ end cleaning enzymes in the mitochondria have been proposed: EXOG, DNA2, and FEN1. EXOG is the only endo/exonuclease that exists exclusively in the mitochondria, and my results suggest that EXOG is the …
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Atm-/-Ung-/- Mice Present Reduced Levels of Switched Immunoglobulin Isotypes IgG1 and IgG3
… AID with apurinic/apyrimidinic endonuclease 1 (APE1), suggesting that ATM may function in BER through p-AID. However, mice possessing a knock-in mutation altering their serine-38 site to an alanine and a <em>Ung</em><em><sup>-/-</sup></em> genetic mutation show a complete block in CSR <em>in …
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More evidence for H2O2-mediated oxidative stress in vitiligo-increased epidermal DNA damage / repair.
… effective DNA repair machinery via hOgg1, APE1 and DNA polymeraseß. Taking into consideration that these patients do not have an increased prevalence for solar-induced skin cancers, our data suggest that BER is a major player in the hierarchy to combat H2O2-mediated oxidative stress …
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H2O2-mediated oxidation and nitration enhances DNA binding capacity / DNA repair via up-regulated epidermal wild-type p53 in vitiligo.
… machinery to mediate DNA repair via hOgg1, APE1 and DNA polymerase ß (Shalbaf 2009). On this basis it is tempting to conclude that DNArepair is the overriding mechanism to combat oxidative stress in this disease.