Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"AP-3"”.
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Characterisation of the AP-3 adaptor-like complex
… coat consists of two components, clathrin and adaptor (or AP) complexes, the AP-1 complex is associated with the clathrin coated vesicles that bud from the TGN and the AP-2 complex is associated with the clathrin coated vesicles that bud from the plasma membrane. A new type of adaptor-like …
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Proteomic analysis of the sorting machineries involved in vesicular traffic between the biosynthetic and endosomal compartments
… focusing on the connection between the Golgi apparatus and the endosomal compartment, mediated by two parallel trafficking pathways regulated by the clathrin adaptors AP-1A and AP-3 (Owen et al., 2004). Typical cargo molecules sorted along the AP-1A regulated pathway are mannose 6-phosphate …
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A Molecular Mechanism for Endocytic Recycling of the M5 Muscarinic Acetylcholine Receptor
… poor receptor immunoreactivity and a wide, overlapping pattern of expression. MRs are characterized by the presence of a large third intracellular loop domain (i3), the sequence of which is divergent between MR subtypes. The i3 is known to determine signaling and trafficking characteristics of …
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Biotin and Fluorescent Labeling of Ribonucleic Acids Using T4 Rna Ligase (Rna Modification, Rna-Dna Hybridization)
… and D. melanogaster 5S RNA, yeast tRNA('Phe), (Ap)(,3)C, and (Ap)(,3)A serve as acceptors with yields of products varying from 50 to 100%. Analysis of the oligoribonucleotide (Ap)(,3)C reactions with RNase A and PNase A plus bacterial alkaline phosphatase demonstrated that a phosphodiester bond …
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Molecular basis of cargo sorting by endosomal trafficking machinery
… marking a critical junction between the Golgi apparatus, plasma membrane and endo-lysosomal pathway. It is at these early endosomal organelles that the sorting of a transmembrane protein determines if it is recycled for further use or degraded. Pathophysiological conditions from pathogens, rare …
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The mechanism of HIV-1 Nef-mediated downregulation of CD4
… clathrin-associated, plasma membrane-localized AP-2 complex in the Nef-mediated downregulation of CD4. In contrast, depletion of the related AP-1 and AP-3 complexes, which direct transport from the TGN and endosomes, had no effect. The requirement for AP-2 was subsequently confirmed in a human …
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Identification of host factors required for cytosol entry by Shigella flexneri: Results of a genome-wide CRISPR/Cas9 screen
… protein KHDRBS1, known to act as a signalling adaptor and regulator of inflammatory responses, facilitates cytosol entry and replication by *Shigella*. I showed that the transcriptional regulator YY1 is also required for cytosol entry and replication. Both KHDRBS1 and YY1 are likely to act …
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Mixed variational problems associated with stationary viscous incompressible free boundary flows
A strategy that is often used in the study of capillary free boundary (FB) problems for viscous incompressible flows is the following: (1) Ignore one of the boundary conditions at the FB and prove that for every chosen position of the FB the resultant problem, here called the auxiliary problem …
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Wechselwirkungen von Proteinen mit den zytoplasmatischen Domänen der Mannose-6-Phosphat-Rezeptoren
… mit den µ2- und µ3A-Untereinheiten der Adaptorkomplexe AP-2 und AP-3 im Hefe Two-Hybrid-System untersucht und durch Kopräzipitationsanalysen verifiziert. Die Wechselwirkung zwischen dem MPR46-CT und µ2 wird durch die C-terminalen Aminosäureste 49-64 der zytoplasmatischen Rezeptordomäne …
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New reporters of protein trafficking and protein-protein interactions in live cells
… biotin to a 15amino acid recognition sequence (AP). Only upon PPI would BirA be in close enough proximity to biotinylate the AP. Although the use of proximity biotinylation within the IF scheme proved challenging because of the geometric rigidity of the antibody conjugates, we later successfully …
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Hexamerine und Neuropeptide in der postembryonalen Entwicklung der Insekten
… Der erste identifizierte Interaktionspartner - d-AP-3 - ist Teil eines Adaptin- Komplexes, der als Adapter zwischen membranständigen Rezeptoren und Clathrin oder ähnlichen Proteinen an der rezeptorvermittelten Endozytose beteiligt ist. Die Adaptin-Interaktionsdomäne liegt innerhalb des …
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Role Of Type Ii Phosphatidylinositol 4-Kinases In Endosomal Tubule Dynamics During Melanosome Biogenesis
… PI4KIIα and PI4KIIβ interact respectively with AP-3 and AP-1, both of which control BLOC-1-dependent cargo sorting. We hypothesized that type II PtdIns-4-kinases support BLOC-1-dependent tubule formation via PtdIns4P to regulate melanosome biogenesis. We found that depletion of either PI4KIIα or …
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The effect of scaffold physical properties on endothelial cell function
… within three-dimensional matrices without recapitulation of the full vessel architecture. Within these matrices, surface and structural properties impose a set of forces on the embedded EC. Indeed, substrata pore size and modulus have profound effects on phenotype and function of a range of …
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Comportamiento de tres cultivares clonales de quequisque (Xanthosoma sagittifolium (L.) Scott) en la Comunidad de la Poma, Masaya, postrera 99-00
… estudio fue el determinar el rendimiento y adaptabilidad de tres cultivares clonales de quequisque Masaya (MY), Nueva Guinea (NG) y Apalí (AP), en la comunidad de La Poma, Masaya establecidas en época de postrera. El ensayo se estableció siguiendo arreglo del diseño de bloque completos al …
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The regulation of yeast homotypic vacuole fusion and membrane trafficking events by sphingolipids, ergosterol, and lipid rafts
… the activity of SNAREs, small GTPases, chaperones, tethering complexes, and specific regulatory lipids. This process occurs in four experimentally defined steps: priming, tethering, docking, and fusion. Work with baker’s yeast identified interdependence between a set of pro-fusion proteins …
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Prevalencia y clasificación de la enfermedad renal crónica en un área de salud de Murcia. Valoración de una intervención al cabo de un año y propuestas de mejora.
… cardiovascular. Desde Atención Primaria (AP) se debe realizar la identificación de población de riesgo, prevención y tratamiento de factores de riesgo, optimización del tratamiento de ERC, remisión con criterio de pacientes a nefrología. Para ello los médicos de familia (MF) de AP deben …