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Showing 1 to 11 of 11 for “"ALKYLTRANSFERASE"”.
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ΜΕΤΑΛΛΑΞΟΓΕΝΕΣΗ ΚΑΙ ΕΝΕΡΓΟΠΟΙΗΣΗ ΤΟΥ RAS ΟΓΚΟΓΟΝΙΔΙΟΥ ΑΠΟ ΑΛΚΥΛΙΩΤΙΚΟΥΣ ΚΑΡΚΙΝΟΓΟΝΟΥΣ ΠΑΡΑΓΟΝΤΕΣ
… BECAUSE IT REPAIRS THE G:T DEMETHYLATION 3) IN ALKYLTRANSFERASE DEFICIENTE. COLI CELLS, THE O6MEG MUTAGENIC EFFICIENCY IS SIGNIFICANTLY INCREASED, THEREFORE THE ALKYLTRANSFERASE IS THE MAJOR REPAIR ENZYME OF THE LESION. HOWEVER, IN THE ABSENCE OF ALKYLTRANSFERASE AND MISMATCH REPAIR, THE MAIN …
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Surface Displayed SNAP as a New Reporter in Synthetic Biology
… allowing us to place human O6-alkylguanine DNA alkyltransferase (hAGT), also known as SNAP, on the surface of a bacterial cell. Initially, we used this synthetic component as a tool for spatially segregating orthogonal synthetic gene outputs by visualizing an extracellular synthetic green …
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Studies on the mechanism of action on antitumour imidazotetrazinones
… of the DNA repair enzymes O6-alkylguanine-DNA-alkyltransferase (O6-AGAT) and 3-methyladenine-DNA-glycosylase (3MAG) have been examined in a range of murine and human cell lines with differential sensitivity to temozolomide. All the cell lines were proficient in 3MAG despite there being 40-fold …
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Validation of an aASPH-SNAP-tag fusion protein for immunodiagnosis of triple-negative breast cancer
… the human DNA repair enzyme O6 -alkylguanineDNA-alkyltransferase (AGT) that has the ability to recognize and bind to any benzyl-guanine modified substrates in a 1:1 stoichiometry. This makes SNAP-tag technology a favourable tool to use for studying the different properties of antibody …
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Development of potential immunodiagnostic & therapeutic techniques using SNAP-fusion proteins as tools for the validation of Triple-negative Breast Cancer
… the human DNA repair enzyme O6-alkylguanine-DNA-alkyltransferase (AGT), which autocatalytically binds benzyl-guanine modified substrates such as fluorophores or small molecule toxins covalently in a 1:1 stoichiometry. In this study, the primary aim was the comparison of different antibody formats …
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Photoimmunotheranostic targeting of CSPG4-positive melanoma cells using SNAP-tag technology
… the human DNA repair enzyme O6-alkylguanine-DNA alkyltransferase. It reacts autocatalytically and in a strictly 1:1 coupling chemistry with substrates that have been modified with benzylguanine (BG). Through genetic fusion of this self-labelling protein with a tumour targeting antibody, we …
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Evaluation of tumour-associated antigens to optically label cutaneous basal cell carcinoma for surgical excision
… a protein tag derived from O6-alkylguanine-DNA alkyltransferase, which would allow for covalent auto-labeling of the corresponding antibody based fusion proteins with benzylguanine-modified (BG) substrates (e.g., fluorophores) under physiological conditions with high efficiency at 1:1 …
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Generation of Glioblastoma specific SNAP based antibody fusion proteins for future radiolabelling application
… of the DNA repair enzyme O6 -alkylguanine DNA alkyltransferase (hAGT), to a single chain variable fragment (scFv) region of an antibody, thereby providing a tool that can detect and label TAAs. The SNAP-tag® element is engineered to irreversibly react with any benzylguanine (BG) substrates, …
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Developing novel SNAP tag-based antibody fusion proteins directionally conjugated to fluorophores for immunophenotyping of acute myeloid leukemia
… of the human DNA repair enzyme alkylguanine-DNA alkyltransferase (AGT), which reacts specifically with O6-benzylguanine (BG)-modified molecules via irreversible transfer of an alkyl group to a cysteine residue within its active site. Benzylguanine can be chemically attached to a variety of …
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Establishment of recombinant antibody technologies allowing for the generation of SNAP-tag fusion proteins
… a human DNA repair enzyme, O6 - alkylguanine-DNA-alkyltransferase (AGT) which naturally removes alkyl residues from damaged DNA. The enzyme reacts specifically with benzylguanine (BG) derivatives via irreversible transfer of alkyl groups to cysteine residues forming stable end products. In this …
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Development of SNAP-tag based fusion proteins as novel auristatin F-containing immunoconjugates and photoimmunotheranostics in the detection and treatment of triple-negative breast cancer
… mutant of the human O(6)-alkylguanine-DNA alkyltransferase, endowed with the ability to specifically and irreversibly react with benzylguanine (BG) derivatives, forming a stable product. Based on the above premises, this research aims to use SNAP-tag technology as a cutting-edge …