Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"AHSV"”.
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Expression and characterization of plant produced AHSV nanoparticles encapsulating EGFP
… protein of African horse sickness virus (AHSV). Plant-based transient expression of the AHSV EGFP-VP3 and AHSV VP7 structural proteins has been used to generate CLPs from N. benthamiana leaves that successfully encapsulate the EGFP protein. However, interaction of this particle with the …
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Characterization and sequence variation of the virulence-associated proteins of different tissue culture isolates of African Horsesickness virus serotype 4
… equines caused by African horsesickness virus (AHSV), is often fatal, although the pathogenic effect in different animals is variable. Current AHSV vaccines are live attenuated viruses generated by serial passage in cell culture. This process affects virus plaque size, which has been considered …
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Characterisation and expression of ORF2 in genome segment 10 of African horse sickness virus
… was identified in African horse sickness virus (AHSV) Seg-10 which encodes the non-structural protein NS3. The ORF2 conservation, size, nature, expression, function and potential subcellular localisation of the putative protein product AHSV is not known and is investigated in this project. A …
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Investigating the production of a particulate plant-produced vaccine candidate against African horse sickness
… known serotypes of African horse sickness virus (AHSV), which is spread by Culicoides midges. Currently, a multivalent live attenuated vaccine is the only vaccine licensed for use in South Africa. However, it has the inherent risk of reverting to virulence as well as the possibility of genome …
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The Development of an African Horse Sickness Virus VP7 Quasi-Crystal Vaccine Candidate in N. benthamiana
… immunised with African horse sickness virus (AHSV) VP7 against a lethal challenge of AHSV-7 (Bailey 2016; Rutkowska et al. 2011; St Clair et al. 1999; Storni et al. 2005; Wade-Evans et al. 1997). This study investigates a safer monovalent vaccine alternative based on plant-produced …
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The characterisation of aspects related to the single stranded RNA binding ability of African horsesickness virus nonstructural protein NS2
African horsesickness virus (AHSV) is a member of the Orbivirus genus and part of the family Reoviridae. It is a double stranded RNA virus with ten genome segments that encode seven structural and four nonstructural proteins. AHSV nonstructural protein NS2, encoded by segment eight, is a single …
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Development of a single real-time RT-PCR method for the group-specific identification of African horsesickness virus and bluetongue virus
… for the specific detection and identification of AHSV and BTV in one reaction, using melting temperatures (Tm) to discriminate between the viruses. Two primers pairs were designed to bind to areas of homology within genome segment 7 (VP7) of AHSV and genome segment 5 (NS1) of BTV respectively. The …
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Development of plant-produced African horse sickness vaccines
… of the orbivirus African horse sickness virus (AHSV) and it is spread by Culicoid midges. The disease has significant economic consequences for the equine industry both in southern Africa and increasingly further afield as the geographic distribution of the midge vector broadens with global …
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Use of baculovirus surface display for characterization of AHSV4-VP2 antigenic structure
… is caused by African horse sickness virus (AHSV), which is mostly endemic in sub-Saharan Africa. However, occasional outbreaks have occurred outside Africa, such as the devastating AHSV-9 outbreak in 1959 reaching India and Pakistan or the European outbreaks caused by serotype 4 (AHSV4) …
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Characterizing the effect of mutation recombination and reassortment on the genetic diversity of African horse sickness virus genomes
African horse sickness virus (AHSV) is a member of the Orbivirus genus within the Reoviridae family and the aetiological agent for African horse sickness (AHS). It is an arthropod-borne virus transmitted by biting midges belonging to various Culicoides spp. Nine antigenically distinct AHSV (AHSV …
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Identifying B-cell epitopes of African horse sickness virus serotype 4 recognised by antisera of immunised horses
… of vaccines and immunodiagnostic reagents. AHSV proteins have been shown to confer protection to model animals in one form or another. This study was undertaken to investigate the global humoral immune reaction during immunisation in horses. This was done using sera from immunised horses and …
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Echocardiographic evaluation of horses challenged with African horse sickness virus
… infected with African horse sickness virus (AHSV) as part of a vaccine trial to describe echocardiographic changes during the course of the disease. Echocardiography was performed once prior to infection, and daily from infection to humane euthanasia of each subject. Both objective and …