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Showing 1 to 20 of 80 for “"AFLP"”.
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AFLP Marker Analysis Of Monoploid Potato
… current potato research. In the present study, AFLP analysis was conducted on two sibling S. phureja "BARD 1-3" monoploid populations derived by androgenesis in anther culture, and gynogenesis through the use of a haploid-inducing pollinator, S. phureja "IVP 101." Little indication of somaclonal …
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AFLP markers linked to Fusarium head blight resistance in Triticum aestivum.
In this study, AFLP technology was used to find markers linked to genes controlling Fusarium head blight (FHB) resistance in Triticum aestivum L. FHB is a disease of cereal crops that results in reduced wheat yields, discoloured, shrivelled kernels, mycotoxin accumulation, and reduced seed and …
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Amplified fragment length polymorphism (AFLP) analysis of genetic variability in Phalaenopsis
Amplified fragment length polymorphism (AFLP) markers allow a rapid assessment of the level of genetic variation that would be difficult to evaluate using a limited number of morphological markers. AFLP was used to assess the level of genetic variation among 16 different Phalaenopsis species and …
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Genetic fingerprints of microalgal culture strains: amplified fragment length polymorphism (AFLP) for investigations below the species level
… von genetischen Fingerabdrücken, die mit der AFLP (amplified fragment length polymorphism) Technik generiert wurden, untersucht. Mittels AFLP was es möglich, verschiedene Stämme derselben Art (Chlorella vulgaris) zu unterscheiden und für jedes Isolat einen charakteristischen genetischen …
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Introgression of the Genes for Sesquiterpene Carboxylic Acid Synthesis From a Wild Tomato, Lycopersicon Hirsutum, Into the Cultivated Tomato for Increased Host -Plant Resistance to Insect Pests
… the Amplified Fragment Length Polymorphism (AFLP) protocol. Progeny of each generation were separated into high and low classes for SCA levels and the DNA combined of individual plants within the phenotypic classes. Bulk segregant analysis was used to identify polymorphic AFLP markers present …
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Molecular Genetic Variation of Diaporthe Phaseolorum and Phomopsis Longicolla and Their Detection From Soybean Tissues
… analysis dendrograms were developed based on the AFLP data using the computer program SAS. Some Diaporthe and Phomopsis isolates from the same host were in different clusters and an individual cluster had isolates from various hosts. In addition, AFLP data demonstrated that genetic variation of P. …
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Molecular Mapping Of A Soybean Mosaic Virus (SMV) Resistance Gene In Soybean (Glycine Max)
… strategy, amplified fragment length polymorphic (AFLP) marker analysis of the two parental lines, NILs and bulk segregants was performed using 64 primer combinations. Initial breakthrough came in the form of AFLP primer combination of Eco+AAC/Mse+CTG exhibited polymorphism between NILs, bulk …
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Population Structure, Aggressiveness, and Molecular Genetic Variability of Sclerotinia Sclerotiorum
… by amplified fragment length polymorphism (AFLP). A dendrogram was calculated utilizing the neighbor joining method within the NTSYS-pc system. Each isolate produced a unique AFLP genotype, and the range in genetic similarity was 0.1022 to 0.3387. AFLP analysis revealed that S. sclerotiorum …
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Genetic Diversity in Native and Invasive <i>Rubus </i>(Rosaceae)
… used; Amplified Fragment Length Polymorphism (AFLP) and Randomly Amplified Fingerprints (RAF). Using three AFLP primers and two RAF primers, genetic similarity was determined and phylograms were constructed. Through statistical analysis and phylogram data it was determined that there might be …
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A Phylogeographic Study of Arenaria ciliata and Arenaria norvegica in Ireland and Europe
… Amplified fragment length polymorphism (AFLP) markers were generated for all populations sampled using three selec- tive primers and the obtained data were analysed using neighbour- joining analysis, networks, AMOVAs and direct analysis of the frag- ments. The results indicate a complex …
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Molecular Genetics on the Genus Glycine: I. Phylogenetic Study of the Subtribe Glycininae. II. Development of a Universal Soybean Genetic Map
… but also on molecular traits such as RFLP, RAPD, AFLP, and SSR data. None of these, however, has been associated with specific chromosomes of soybean. Therefore, this study was conducted with the objective of integrating all available classical genetic i.e. pathological, morphological, and …
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Genetic and Molecular Analysis of the Rj(7) Gene in Soybean Using Pcr-Based Technologies
… and amplified restriction length polymorphism (AFLP) technologies were used for molecular genetic studies. The bulked segregant analysis and segregation analysis were performed for the detection of DNA markers. The results indicated that among the marker systems used there was no SSR marker …
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Identification of genes related to self-incompatibility in ryegrass (Lolium perenne L.)
… two differential expression techniques: cDNA-AFLP and the cDNAmicroarray. The genome-wide expression analysis carried out by cDNAAFLP allowed us to identify 479 TDFs (Transcript Derived Fragments) as putatively related to SI in L. perenne. All the expression profiles were quantified using AFLP …
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Species-level phylogenetic reconstruction of the African cycad genus Encephalartos (Zamiaceae)
… between different taxa. In this study, AFLPs were used together with DNA sequencing to reconstruct the phylogenetic relationships of the genus. This study is the first to be presented with aims of resolving the relationships of Encephalartos using AFLPs together with DNA sequences. Total …
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Towards a molecular approach for the identification of fungal taxa that contain psilocybin
… of amplified fragment length polymorphism (AFLP) produced by five selective primer pairs were also analyzed. By the analysis of genetic similarity, RAPD and AFLP method were capable of providing genus and species information. AFLP band patterns can provide reliable species test and can be …
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Aronia mitschurinii: Solving a Horticultural Enigma
… using amplified fragment length polymorphic (AFLP) analysis. Successful seed formation was achieved between maternal diploid <em>A. melanocarpa </em>and <em>Malus domestica</em>, <em>Photinia serrulata</em>, <em>Sorbus</em>, and ×<em>Sorbaronia</em>. Clustering of AFLP similarity data using …
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Spatial structuring in trait variation in Polyommatus icarus in a functional context
… with allozymes and a neutral DNA marker (AFLP) variation are studied here to understand how geographic trait variation is related to environmental variation. The approach adopted here is to study this along a latitudinal cline of temperature and photoperiod, using four populations from …
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Taxonomical use of floral scent data in apomictic taxa of Hieracium and Sorbus derived from hybridization
… between the taxa involved. In Sorbus additional AFLP (Amplified Fragment Length Polymorphism) analyses were carried out from the populations and individuals that were studied for scent and genetic and scent data were correlated. In Hieracium, the inflorescense scent consisted mainly of …
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Population Genetics and Differential Pathogenicity of the Soybean Brown Stem Rot Pathogen Phialophora Gregata
… between genetic variation and pathogenicity. AFLP (amplified fragments length polymorphism) and ISSR (inter simple sequence repeat) analyses were used to detect genetic diversity in Phialophora gregata. Various levels of DNA polymorphism were detected among the 45 soybean and mung bean …
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