Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"ADP-Ribosylation"”.
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Regulation of Protein Degradation by ADP-Ribosylation
… The post-translational modification, poly-ADPribosylation has an estimated half-life of only 1-6 min. My thesis research aimed to reveal how the substrates of Tankyrase are degraded rapidly. Using Drosophila melanogaster, I described the role of Iduna E3 ubiquitin ligase in the regulation …
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Development and Application of Chemoenzymatic Tools to Investigate Site-Specific Protein ADP-Ribosylation
… the first technologies to install mono- and poly-ADP-ribosylation at specific sites on synthetic peptides and full-length proteins. Historically, chemical and topological complexities associated with ADP-ribosylation have stymied progress in this area. To overcome this, I have identified enzymes …
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Analysis of Interrelationships Among NAD+, PARP1, ADP-Ribosylation, and Splicing in Murine Embryonic Stem Cells
… Previous studies have implicated poly(ADP-ribose) polymerase 1 (PARP1), an abundant nuclear enzyme that plays key roles in a variety of nuclear processes, in ESC self-renewal and lineage commitment. Given the diverse molecular functions of PARP1, I sought to determine the potential …
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Study of the Cellular Role of GRP78/BiP mono-ADP-ribosylation in UPR and Cancer
Mono-ADP-ribosylation is a reversible post-translational protein modification that modulates the function of proteins involved in different cellular processes, including signal transduction, protein transport, transcription, cell cycle regulation, DNA (deoxyribonucleic acid) repair and apoptosis. …
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Chemical Genetic and Genomic Discovery of PARP-1-Dependent Mechansims of Transcriptional Regulation
Poly(ADP-ribose) polymerases, or PARPs, are a family of enzymes that modulate diverse biological processes through covalent transfer of ADP-ribose from NAD+ onto target proteins. These targets of post-translational modification as well as the genomic targets, or binding sites, to which the nuclear …
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Investigating the Role of PARylation in Regulating Skeletal Muscle Mass and Function in Healthy Mature Mice
Adenosine diphosphate (ADP) ribosylation is a post-translational modification dependent on the transfer of ADPr units from nicotinamide adenine dinucleotide (NAD+) on to a plethora of biomolecules (i.e., proteins, DNA, RNA, etc.) in response to physiological stressors (i.e., nutrient deprivation, …
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DEVELOPING SMALL-MOLECULE PROBES AND INHIBITORS FOR ADP-RIBOSE-BINDING PROTEINS
… (PTMs), protein adenosine diphosphate ribosylation (ADP-ribosylation) serves as key effectors and regulators in a plethora of biological processes. Although the biological roles of mono-ADP-ribosylation (MARylation) and poly-ADP-ribosylation (PARylation) have been extensively studied, …
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Novel interactions of the 5-HT₂ₐ and related receptors with intracellular signalling proteins
… through the phospholipase D (PLD) pathway in an ADP-ribosylation factor (ARF)-dependent manner, that appears to be independent of Gq/n. The M3 muscarinic receptor, another member of the group I GPCRs, has also been shown to signal through both PLC (via Got) and the alternative pathway of PLD …
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Studies of proteins implicated in the folding and processing of tubulin
… structures being produced. One is of the ADP ribosylation factor like 1 (ARL1) and another of the CAP-Gly domain of tubulin binding cofactor B (TBC-B). Enzymes involved in posttranslational modifications of tubulin, tyrosine ligation and deacetylation, were also investigated. These …
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Acute Nitrate Exposure Causes Proteomic Changes Consistent with the Regulation of Reactive Oxygen and Nitrogen Species
… are involved in G-protein signaling (Rho-GDI, ADP-ribosylation factor, G-protein ß-subunit), protein homeostasis (heat shock protein 70, prohibitin, calreticulin, and proteasome &#;-type 4 subunit), glycolysis (enolase), transport of hydrophobic molecules (lipocalin) and cytoskeletal …
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Enzymatic synthesis of defined-length poly(ADP-ribose) and the investigation of cell-permeable poly(ADP-ribose) glycohydrolase inhibitors
Poly(ADP-ribosylation) (PARylation) is an important post-translational modification that maintains genomic stability in a cell. Engaging in important cellular processes such as DNA repair and cell death signaling, PARylation has gathered considerable interest as a target for genotoxic chemotherapy …
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An Investigation into the Role of Arf6 in MCH-mediated Rearrangements in 3T3-L1 Preadipocytes
… that is required in the subsequent activation of ADP-ribosylation factor 6 (Arf6). These events in succession may result in destabilized actin in murine 3T3-L1 pre-adipocytes. A pharmaceutical inhibitor of Arf6, NAV-2729, as well as the overexpression of dominant negative ARNO/Arf6 plasmids were …
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No-Independent Modulation of Soluble Guanylyl Cyclase (Sgc) Activity and Function
… For example, the interaction of sGC with ADP-ribosylation factor GTPase activating protein 1 (AGAP1) governs sGC’s intracellular distribution and therefore mediates localized production of cGMP. Interactions of sGC with heat-shock protein 90 (HSP90) or HSP70 promote the extent of sGC …
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NcCyP41, a two domain neurospora crassa cyclophilin : characterization of its peptidyl-prolyl cis/trans isomerase activity ; isolation and functional analysis of two novel NcCyP41-binding proteins
… spectrometry analysis has identified a potential ADP-ribosylation of CyPBP37. <br> <br>CyPBP37 is an abundant protein which is highly expressed in the logarithmic and the early stationary phase but only in thiamine free medium. The protein disappeared progressively from early to late stationary …
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Characterization of Novel Substrates of the Tankyrase and RNF146 Destruction Complex and Mechanisms of its Regulation
Poly-ADP-ribose is a post-translational modification that was first described over 50 years ago as a polymer derived from nicotinamide adenine dinucleotide or NAD. Since then, a family of 17 enzymes responsible for generating Poly-ADP-ribosylation or PARylation post translational modification has …
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PI(4)P-Dependent Recruitment of Clathrin Adaptors to the Trans-Golgi Network
… TGN is absolutely dependent on the small GTPase ADP ribosylation factor 1 (Arf1), but paradoxically, Arf1 has a broader intracellular distribution than these adaptors. We found that the Golgi is particularly enriched in phosphatidylinositol 4 phosphate [PI(4)P] and that the clathrin adaptor AP-1 …
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Trimming Fat Upon Infection: Proteolytic Demyristoylation as a Novel Bacterial Pathogenic Strategy
… host proteins, it strongly favors members of ADP-ribosylation factors family (ARF) and related ARF-like (ARL) proteins. By inactivating ARF and ARL proteins, IpaJ disrupts the Golgi structure and prevents secretion through general secretory pathway. I also found that by blocking protein export …
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Signal transduction by the 5-HT2A receptor and its H452Y polymorphic variant
… D (PLD) via recruitment of the small G-protein ADP-Ribosylation Factor (ARF). This thesis focusses on protein:protein interactions and signalling mechanisms of the 5-HT2AR and H452Y-5-HT2AR receptor variant. Both ARF1 and PLD1 have previously been shown to bind to the carboxy-terminal tail (ct) …
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Toward a Quantitative Analysis of PARP-1 and Poly(ADP-ribosyl)ation in Cellular Senescence
… evidence to suggest a connection between poly(ADP-ribose) (PAR) and mammalian longevity, no studies have been done to explore a possible role for PARP-1 — the enzyme responsible for synthesis of 90% of cellular PAR — in mechanisms of senescence. Furthermore, many techniques currently used for …
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Postsynaptic Cholinergic Control Of Cardiac Function In Aging
… protein measured by Western immunoblotting and ADP-ribosylation techniques was significantly greater in the atria and ventricles of the aged compared to adult rats. The fidelity of the signal transduction through muscarinic receptor-linked Gi protein, as judged from the guanine …
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