Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 59 for “"ACE2"”.
-
Development of targeted gene delivery vectors to assess cardiac overexpresion of ACE2 in vivo
… ones. This thesis aimed to assess the effect of ACE2 overexpression in vivo on heart function and blood pressure. In order to achieve cardiac gene transfer, we first had to identify an efficient cardiac gene delivery vector. This was approached by the application of the two main techniques; (1) …
-
Development of Novel PET Radiotracers for Neuroinflammation and COVID-19: Targeting FPR2, I2BS and ACE2
… (I2BS) and angiotensin-converting enzyme 2 (ACE2) via PET. These molecular targets are important to understand the pathological processes in neuroinflammation and COVID-19. Over the course of project 1, automated radiosynthetic methods were developed to produce [11C]Quin C1 and [18F]Quin …
-
Investigating the Unique Mechanism of Action of CADA Analogs for Down-Modulating CD4, Sortilin, and ACE2
… the down-modulation of several proteins (CD4, ACE2, sortilin) with the establishment of a pharmacokinetic profile of various biologically active CADA analogs. To achieve these goals, deuterium-labeled CADA analogs (CK147 and TL020) and several signal peptides (hCD4, mCD4, Q19A mCD4, Q16A Q19A …
-
Structural Basis of Bat and Raccoon Dog Angiotensin-Converting Enzyme 2 (ACE2) Receptor Recognition by Sarbecoviruses
… binds human angiotensin-converting enzyme 2 (ACE2) with high affinity despite limited apparent time for adaptation, and why some bat-derived RBDs paradoxically bind human ACE2 more strongly than bat ACE2. To address this question, we compared the RBDs of SARS-CoV-2 and BANAL-52, a related bat …
-
Developing novel prophylactic approaches against SARS-CoV-2 infection
… the modulation of viral host receptors, such as ACE2, could represent a new chemoprophylactic and therapeutic approach for COVID- 19 complementing vaccination. However, the mechanisms controlling ACE2 expression remain elusive, partly due to low ACE2 levels in experimental models. To address this …
-
Exploring the therapeutic potential of the apelin receptor signalling axis, and angiotensin-converting enzyme 2 (ACE2) as the SARS-CoV-2 viral entry receptor, in the cardiovascular system
… pointing to loss of pharmacological function. ACE2 is a zinc-metalloproteinase type 1 transmembrane protein that cleaves angiotensin peptides to counter-regulate the renin-angiotensin-aldosterone system in the cardiovasculature and mediate hypotensive responses. The protein also acts as the …
-
Human testis angiotensin-converting enzyme: crystal structure of a glycosylation mutant and investigation of a putative hinge-mechanism by normal mode analysis
… (tACE), and homologues Drosophila AnCE and human ACE2. tACE is identical to the C-terminal domain of somatic ACE, which consists of two homologous domains, each having a slightly different substrate-specificity. This thesis describes the purification, crystallisation and X-ray crystal …
-
Protein and peptide-based biomaterials for treatment of COVID-19, crossing the blood-brain barrier and local delivery of antibiotics
… of the dissertation is to discover anti-ACE2 peptides. In Chapter 1, we briefly discussed the COVID-19 virus and its infectivity. We then discussed the challenges of delivering therapeutics to the brain and the need to discover BBB penetrating peptides. We also briefly described the need …
-
INFECTION OF MACROPHAGES BY SARS-COV-2: IMPLICATIONS FOR VIRAL SPREADING AND IMMUNE RESPONSE
… of the conventional viral entry protein ACE2, but also report evidence for ACE2-independent entry pathways in macrophages, as anti-ACE2 monoclonal antibodies efficiently blocked viral infection in conventional epithelial cells but not in this new SARS-CoV-2 target cell.
-
Antibody production with synthetic peptides against human Coronavirus-NL63 ORF3 protein
… (S1 domain) use angiotensin‐converting enzyme 2 (ACE2) for infectious entry. Furthermore, CoV‐NL63 simplifies binding to ACE2 by attaching to heparan sulphate proteoglycans on the cell surface. ACE2 has a significant expression in airway differentiated epithelial cells and CoV‐NL63 enter these …
-
New Advances on the Biochemical Pathways in the Reninangiotensin System in Hypertension and their role in Cardiac Structure and Function
… first that angiotensin converting enzyme 2 (ACE2) directly converts angiotensin (Ang) II into Ang‐(1‐7) only in hypertrophic hearts isolated from hypertensive rats. Moreover, chronic in vivo pharmacological blockade of ACE2 xvi resulted in a disruption of the balance of cardiac Ang II and …
-
Mechanisms of Emerging RNA Virus Adaptation to Hosts
… the entry of pseudotyped viruses in human ACE2 (hACE2)-expressing cells and decreased replication in human lung cells through reduced hACE2 binding. Next, serial passaging of SARS-CoV-2 in cells expressing the animal ACE2 receptor identified the recurring mutation A222V in the Spike gene. …
-
Computationally Designed Peptide Binder and Molecular Beacon for SARS-CoV-2
… and experimental validation to develop an ACE2-derived peptide that targets the viral spike protein receptor-binding domain (RBD). The peptide was derived utilizing the existing crystal structure of spike protein’s RBD and ACE2 to determine the linear peptide fragments that contributed the …
-
Characterization of a novel mouse model and vaccines for pulmonary pathogens
… of a new gene replacement mouse model (ACE2-GR) for COVID-19. Mice were productively infected with SARS-CoV-2, displayed no symptoms, and developed mild viral pneumonia prior to recovery. These findings establish ACE2-GR mice as a physiologically relevant model for mild disease. The …
-
Profiling Host Respiratory Responses to SARS-CoV-2 Infection
… and discover that the SARS-CoV-2 entry receptor ACE2/Ace2 is an interferon-stimulated gene in human but not murine nasal epithelia. We then profile nasopharyngeal swabs taken from individuals upon hospital admission, stratifying epithelial responses to infection based on ensuing peak disease …
-
Consequences and Limits of Cell-Cell Communication in Airway Immune Responses
… highest expression of the SARS-CoV-2 receptor, ACE2, and provide evidence that the ACE2 mRNA gets upregulated during interferon responses. Finally, we investigated whether airway epithelial cells send secondary signals to one another after sensing immune-relevant signals like cytokines. To do …
-
SARS-CoV-2 Variants of Concern: Biological Implications of Immunogenicity and Comorbidity
… for SARS-CoV-2, angiotensin-converting enzyme 2 (ACE2). As the infection of the K18 hACE2 mouse model with SARS-CoV-2 results in lethality, a dose needed to be chosen that the mouse survives in order to examine protection conferred by an initial infection. The mice that survived the SARS-CoV-2 WA1 …
-
Regulation of Substrate Metabolism and Renin-Angiotensin System with Dietary and Pharmacological Interventions: Implications for Obesity and Related Disorders
… an increase in angiotensin converting enzyme 2 (ACE2), the receptor for SARS-CoV-2 cellular infection. We observed that the obesogenic, high-fat sucrose-enriched (HFS) diet elevated the pulmonary content of this receptor, whereas the KD did not alter ACE2 levels. Moreover, the KD significantly …
Page 1 of 3