Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 21 for “"AAA-ATPases"”.
-
The molecular basis of LINC complex formation
… also arise from the structure. TorsinA is an AAA+ ATPase suggested to play a role in LINC complex regulation. Analysis of Torsin's binding partners LAP1 and LULL1 show that they are catalytically inactive AAA+ ATPases. We characterize the complex and show by EM that they form ring akin to …
-
Sequential Actions of VCP/p97 and the Proteasome 19S Regulatory Particle in Sterol-Accelerated, ER-Associated Degradation of HMG CoA Reductase
… through poorly defined reactions mediated by the AAA-ATPase VCP/p97 and augmented by the nonsterol isoprenoid geranylgeraniol. Here, we report that the oxysterol 25-hydroxycholesterol and geranylgeraniol combine to trigger extraction of reductase across ER membranes prior to its cytosolic release. …
-
Single molecule fluorescence spectroscopy of ClpXP-mediated substrate degradation
… in prokaryotes and organelles of eukaryotes. AAA+ ATPases in these proteases recognize protein substrates and power their mechanical denaturation and subsequent translocation into a sequestered degradation chamber where polypeptide cleavage occurs. Here, we present the single molecule …
-
Mechanistic studies of a AAA+ protease
AAA+ proteases are present in all branches of life and responsible for the energy-dependent degradation of most cytosolic proteins. Substrates for AAA+ proteases are unfolded and translocated into a compartmental peptidase. The requirement for protein unfolding raises several questions. How easily …
-
Design framework of the MuA remodeling signal that confers preferential complex disassembly by the AAA+ unfoldase ClpX
… non-native states. The Clp/Hsp100 family of ATPases are unfolding chaperones that remodel macromolecular complexes and facilitate ATP-dependent protein degradation. They are members of the superfamily of AAA+ enzymes (ATPases Associated with various cellular Activities), which is conserved …
-
Examination of two remarkable AAA+ proteases: unraveling substrate-enzyme interactions of the double-ringed ClpAP and direct thermal activation of HslUV proteolysis
AAA+ (ATPases associated with diverse cellular activities) proteases are degradation enzymes, which together with other molecular machines, including disaggregases and chaperones, are critical to the maintenance of cellular protein homeostasis. Different members of this quality control network …
-
An Investigation of TorsinA Interaction Partners
TorsinA is a AAA+ (ATPases associated with a variety of cellular activities) protein that is implicated in the neuromuscular disorder DYT-TOR1A early-onset isolated dystonia. DYT-TOR1A is a heritable form of dystonia characterized by involuntary twisting movements and postures that arise during …
-
Functional and structural studies of AAA+ proteases
AAA+ proteases are found in all domains of life. They degrade misfolded proteins as well as specific regulatory factors and thus play critical roles in protein quality control and numerous cellular processes. These enzymes share a conserved architecture in which a hexameric AAA+ ATPase recognizes, …
-
Using Small Molecule Tools to Study ATPase Mechanoenzymes
<p>The dysregulation of AAA (ATPases associated with diverse cellular activities) mechanoenzymes has been linked to diseases, and chemical inhibitors and activators can be powerful tools to probe mechanisms and test therapeutic hypotheses. However,the structural conservation across the AAA protein …
-
Communication & coordination between components of the ClpAP degradation machine
AAA+ (ATPases associated with diverse cellular activities) proteases are present in all kingdoms of life. These molecular machines perform energy-dependent regulated proteolysis. The bacterial enzyme ClpA is a double-ring hexameric AAA+ unfoldase/translocase that functions with the tetradecameric …
-
Noncanonical recognition and degradation of a stable soluble protein by AAA protease FtsH
AAA+ (ATPases associated with various cellular activities) proteolytic machines help maintain and adjust the cellular proteome in response to stress or changes in nutrients. AAA+ proteases bind degradation targets and utilize ATP-powered conformational changes in the AAA+ unfoldase ring to denature …
-
Structural Principles of Substrate Recognition and Unfolding by the ClpAP and ClpXP AAA+ Proteases
… Found in all domains of life, proteases of the AAA+ (ATPases associated with diverse cellular activities) superfamily perform targeted protein degradation of specific substrates. All AAA+ proteases consist of a hexameric AAA+ unfoldase and a compartmentalized peptidase. AAA+ proteases harness …
-
Identifying the requirement and mechanism of the dynamic yet kinetically stable ClpXP interface
AAA+ (ATPases Associated with various cellular Activities) proteases carry out regulated degradation and protein quality control. To achieve these goals, a barrel-shaped peptidase, with active sites in a sequestered chamber, cooperates with an attached AAA+ hexamer that recognizes, unfolds and …
-
Mechanisms of CipS reprogramming of the AAA+ protease CipAP
… occur. These peptidase chambers can partner with AAA+ (ATPases Associated with various cellular Activities) unfoldases, protein machines that control substrate access to this chamber. An additional level of control is provided by adaptor proteins, which modulate substrate selection by the AAA+ …
-
Stress-Induced Targeting of Molecular Chaperones In The Yeast Saccharomyces Cerevisiae
… Hsp104 is a member of the Hsp100 superfamily of AAA+ ATPases. Unlike the Hsp90 family of chaperones, Hsp104 is not restricted to a specific set of client proteins, but rather assists in reactivating stress-denatured proteins by solubilizing protein aggregates. I have discovered that Hsp104, along …
-
Lon degrades stable substrates slowly but with enhanced processivity, redefining the attributes of a successful AAA+ protease
… proteins. Mechanoenzymes belonging to the AAA+ (ATPases associated with diverse cellular activities) superfamily are present in all domains of life and harness energy from chemical fuels to perform mechanical work by promoting conformational changes in other biological macromolecules. …
-
Using Chemical Probes to Examine Cellular Activities
… spastin's role in cell division. Spastin is a AAA (ATPases associated with diverse cellular activities) protein that is recruited in anaphase by the endosomal sorting complex required for transport (ESCRT) to the reforming nuclear envelope, where spastin is proposed to sever stable …
-
Structural and functional studies of the ATP-dependent Clp proteases in cyanobacteria
… proteases, of which one major contributor is the AAA+ (ATPases Associated with diverse cellular Activities) family that includes the Clp proteases. The Clp protease exists in many forms of life, ranging from eubacteria to mammals. In the bacterium E. coli, the hexameric ATPases ClpX and ClpA …
-
Biochemical and Structural Insights into VCP Unfoldase and WRN Helicase Complexes
… Cdc48 in yeast) is an essential member of the AAA (ATPases associated with diverse cellular activities) protein superfamily. As its family name suggests, VCP function is implicated in a broad range of biological pathways including protein degradation, DNA repair, and organelle membrane …
-
Cross-Pathway Control of the Pathogenic Fungus <i>Aspergillus fumigatus</i>: a Manifold Stress Response System
… Unter anderem wurden dabei zwei sog. AAA-ATPasen entdeckt, die aufgrund der Tatsache, dass sie Teil des CpcA-abhängigen Transkriptoms sind, als cdaA und cdaB bezeichnet wurden. Entsprechende Mutantenstämme zeigten als Phänotyp eine ausgeprägte Sensitivität gegenüber dem Zellwandstress …
Page 1 of 2