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Showing 1 to 10 of 10 for “"AAA+-Protein"”.
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Chemical Biology, Biochemical and Structural Studies of MDN1, an AAA Protein Required for Ribosome Biogenesis
<p>Cellular proteins are synthesized by ribosomes, which are ~3 MDa macromolecular complexes comprised of four ribosomal RNAs and ~80 ribosomal proteins in yeast. The biogenesis of such complicated ribonucleoprotein complexes is a highly regulated, multistep process requiring a plethora of more …
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Rubisco Activase -- a Typical AAA+ Protein With Unique Features, And, PIFI -- a Novel Chloroplast Protein Functioning in Chlororespiration
A novel Arabidopsis chloroplast protein, named PIFI, with a similar C-extension as activase was identified as functioning in chlororespiration and possibly cyclic electron transport by reverse genetics. In a pifi mutant, which possesses an intact NDH complex, NDH-mediated dark reduction of …
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Using Small Molecule Tools to Study ATPase Mechanoenzymes
<p>The dysregulation of AAA (ATPases associated with diverse cellular activities) mechanoenzymes has been linked to diseases, and chemical inhibitors and activators can be powerful tools to probe mechanisms and test therapeutic hypotheses. However,the structural conservation across the AAA protein …
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Role of the MecA adaptor protein in regulation of the AAA + chaperone ClpC of Bacillus subtilis
ClpC, a member of the AAA+ protein superfamily from Bacillus subtilis, is forming with ClpP a proteolytic system, that is part of the protein quality control system and involved in general proteolysis of misfolded and aggregated proteins. In addition ClpCP together with the adaptor MecA is …
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Untersuchungen zur Struktur und Substratspezifität des AAA+-Chaperons ClpB in Escherichia coli
… und der Substratspezifität des AAA+ Chaperons ClpB aus dem gram-negativen Modellorganismus Escherichia coli. <br>Zur Untersuchung der Struktur von ClpB wurde die Methode der limitierten Proteolyse verwendet. Daran schloß sich eine massenspektrometrischer Analyse der Fragmente an …
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Single-molecule and ensemble studies of DNA replication system
… to reconstitute the DNA replication machinery protein by protein. In the first study, by developing a real-time fluorescence assay, we discovered that RFC can assemble a PCNA ring from monomers in solution. A motion-based DNA polymerization assay showed that the PCNA assembled by RFC is …
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Communication & coordination between components of the ClpAP degradation machine
AAA+ (ATPases associated with diverse cellular activities) proteases are present in all kingdoms of life. These molecular machines perform energy-dependent regulated proteolysis. The bacterial enzyme ClpA is a double-ring hexameric AAA+ unfoldase/translocase that functions with the tetradecameric …
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Mechanisms of substrate recognition by the AAA+ protease HslUV
Protein degradation is a central component of all biological processes. The proteome must constantly change in response to environmental stimuli. As a result, protein synthesis and regulated proteolysis are vital to cell survival. In Escherichia coli, the protease HslUV is one of five ATP-dependent …
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Analyzing Resistance to Design Chemical Inhibitors of AAA Proteins
… As selective binding of inhibitors to their protein targets is mediated by specific interactions of the inhibitor with the protein backbone and side chains, mutations that disrupt these interactions can lead to resistance. Identifying resistance-conferring alleles can thus reveal and suggest …
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Chemical Approaches to Dynein Inhibition
… to maintain order within the cytoplasm. Motor proteins are the enzymes that convert the chemical energy contained in adenosine triphosphate (ATP) into directed movement along polarized filaments of actin and tubulin within cells. Dyneins are the primary enzymes that drive motion toward the …