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Showing 1 to 14 of 14 for “"AA3"”.
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Production and characterization of two AA3 enzymes from Arabidopsis thaliana
… enzymes. Within this class, the AA3 family, contains FAD-dependent oxidoreductases with diverse catalytic roles in fungi, where they participate in lignocellulose degradation. However, the biological functions of their plant counterparts remain largely unknown, and only a few …
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The Study of Aa3-Type Cytochrome C Oxidase in Rhodobacter Sphaeroides
Cytochrome c oxidase is the final electron acceptor in the respiratory chain and catalyzes the highly exergonic oxygen reduction reaction to water and forms a transmembrane electrochemical proton gradient. This transmembrane gradient is used by ATP synthase to produce ATP. The oxygen chemistry …
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The study of aa3-type cytochrome c oxidase in Rhodobacter sphaeroides
Cytochrome c oxidase is the final electron acceptor in the respiratory chain and catalyzes the highly exergonic oxygen reduction reaction to water and forms a transmembrane electrochemical proton gradient. This transmembrane gradient is used by ATP synthase to produce ATP. The oxygen chemistry …
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Study of the Proton Pumping Mechanism of the Aa3-Type Cytochrome C Oxidase
… (O2 + 4e- + 4H+ → 2 H2O). In cytochrome aa3 oxidase (C cO), electrons from cytochrome c are first transferred to a copper center, CuA, which is located on the electrically positive (P) side of the membrane, then transferred through low spin heme a to a binuclear center (high spin heme a3 …
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Studies on Critical Proton Pathway Residues in the Aa3-Type Cytochrome C Oxidase From Rhodobacter Sphaeroides
Aerobic organisms derive energy needed to sustain life from foodstuffs through the process of cellular respiration. Respiration is performed by a series of soluble and multi-subunit membrane proteins that form the 'electron-transport chain', found in the inner mitochondrial membrane of eukaryotes …
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Studies on critical proton-pathway residues in the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides
Aerobic organisms derive energy needed to sustain life from foodstuffs through the process of cellular respiration. Respiration is performed by a series of soluble and multi-subunit membrane proteins that form the ‘electron-transport chain’, found in the inner mitochondrial membrane of eukaryotes …
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Quinol activity of cytochrome aa3-600 menaquinol oxidase from Bacillus subtilis characterized by biochemical and EPR methods
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2018-05-01
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The AA3 Type Cytochrome C Oxidase From Rhodobacter Sphaeroides: Insights Into the Proton Pumping Mechanism From the N139D Mutation in the D-Channel
Cytochrome c oxidase, an integral membrane protein, is the final enzyme of the electron transport chain. The two sides of the membrane are denoted as the N side (the cytoplasmic side in bacteria and the matrix side of mitochondria in eukaryotes) and the P side (the periplasmic side in bacteria and …
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Microscopic description of gas permeation and delivery pathways in biological macromolecules
… terminal oxidases (cytochrome ba3 and cytochrome aa3), nitric oxide reductase (cNOR) and bacterial carboxysome, and to describe the movement of O2, CO2 and NH3 through lipid membranes and membrane channels. In the first part of this dissertation, I illustrated the necessity of pathways in assuring …
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Biochemical characterization of a-type heme-copper oxidases in escherichia coli, bacillus subtilis and thermus thermophilus
… proton pumping. Thermus thermophilus cytochrome caa3 oxidase, a member of A2 subtype, was successfully expressed, purified and characterized by site-directed mutagenesis, especially on YS pair as well as around the region of glutamate. It is suggested that the conserved YS pair in A2-type is a …
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Structural and biochemical studies on enzymes involved in natural product biosynthesis and cellular respiration
… We carried out structural studies on the aa3-menaquinone oxidase, which has high sequence similarity with ubiquinone oxidase from E. coli. We believe structural information on the aa3 can elucidate the mystery of the quinone/menaquinone binding site, which is critical for the q cycle in …
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Characterization of alternative enzymes in bacterial respiration
… but it was instead found to co-purify with the aa3 oxidase as a stable supercomplex. This supercomplex was characterized and its components were identified. The supercomplex was initially isolated in the detergent, dodecyl maltoside (DDM), but did not have a homogenous population. Therefore, …
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Synthese und Screening einer kombinatorischen Rezeptorbibliothek für biologisch relevante Tetrapeptide
… oder zwei Lysinen an Position AA1, AA2 oder AA3 die höchsten Bindungskonstanten aufwiesen. Der Faktor der Bindungskonstanten zwischen sehr gut bindenden Rezeptoren und schlecht bindenden Rezeptoren betrug für beide Peptide über 200! Die besseren Bindungseigenschaften von …
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The interactions of cytochrome bo3 from Escherichia coli with its substrates - ubiquinone and oxygen
Heme-copper respiratory oxygen reductases reduce O2 to water and use the redox free energy to generate the proton motive force. Among the heme-copper oxygen reductases are the quinol oxidases, which catalyze the 2-electron oxidation of ubiquinol or menaquinol instead of cytochrome c. Escherichia …