Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"A549 cell line"”.
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A study of gene expression in human normal and carcinogenic cell lines using qRT-PCR
… the tumour to develop depcnds on activation of cell immortalisation pathway. The correlation of these pathways has not been reported. The present study described the correlation in transcription of genes of two phases of drug metabolism pathways and immortalisation pathway in four lung cell …
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Epidermal growth factor-mediated activation of the unliganded glucocorticoid receptor in A549 cells
… of interleukin 6 (IL-6) mRNA occur in other cell types and whether the effects of EGF are mediated via the GR. This study provides the first evidence of a single ligand (EGF) causing ligand-independent GR activation, resulting in the regulation of cytokine genes in the lung epithelium …
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Synthesis and evaluation of novel heterocyclic compounds as anticancer agents
… is a protein found in all eukaryotic cells. This protein is an essential component formitosis and has several different binding sites at which a variety of chemically differentagents interact. These binding sites include the colchicine, vinca alkaloids, rhizoxin/maytansine and tubulin …
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DRY POWDER INHALATION OF PNEUMOCOCCAL VACCINE USING POLYMERIC NANOPARTICLES AS CARRIERS
… to be effectively taken up by the lung dendritic cells (DCs) were successfully produced. The NPs were then surface adsorbed with PspA4Pro (~20 µg of PspA4Pro per mg of NPs) and spray-dried using L-leu as a microcarrier to produce NCMPs with a product yield of 55.55±6.64% for the PspA4Pro adsorbed …
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PULMONARY DELIVERY OF PNEUMOCCOCAL VACCINE USING NANOCOMPOSITE MICROPARTICLE CARRIERS VIA DRY POWDER INHALATION
… and MMAD of 1.49±0.29 μm. Further cell viability studies on A549 cell line showed a good profile with a cell viability of 79±4.7% for anionic NPs/NCMPs and 78.85±9.96% for cationic xviii NPs/NCMPs at 2.5 mg/ml concentration after 24 h exposure. The previous results introduced a …
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Disentangling bacterial virulence mechanisms: Klebsiella pneumoniae targets post-translational modifications (PTMs) to manipulate innate immunity
… unconjugated ISG15 levels in infected epithelial cells (A549 cell line). Klebsiella was found to reduce isg15 transcription by blocking the phosphorylation of ISG15’s transcription factor IRF3. Remarkably, Klebsiella targets two PTMs to achieve this, namely phosphorylation and ubiquitylation. …
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Identification of determinants of sensitivity to duocarmycin analogues: A class of potent anti-cancer drugs
… involved in the intrinsic sensitivity of cancer cells to duocarmycins. This thesis aims to identify the determinants of sensitivity of duocarmycin analogues by utilising three independent approaches. First, a hypothesis-driven approach was based on literature that suggested an alternative …
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Pharmacological modulation and manipulation of cancer drug resistance
… were successful P-gp modulators in the DLKP-A cell line; the ditrifluoroacetyl resveratrol derivative, RBM15, and the macrocycle derivative, KG104. A panel of nine therapeutic agents were evaluated for their potential to down-regulate multidrug resistant protein expression and thus, overcome …
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In vitro analysis of volatile organic compounds in search of potential biomarkers of lung cancer
… them. Studieshave shown that various cancer cells in vitro produce or consume specific VOCs that can serve as potential biomarkers to differentiate them from non-cancer cells. The present study aimed at the detection, identification and semi-quantification of VOCsreleased or consumed by the …
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Using high-content subcellular proteomics to identify x-ray-induced protein trafficking
… Earth, which pose minimal threat to organisms as cells have multiple mechanisms to prevent damage or death. However, high levels of IR can overwhelm these mechanisms causing a cascade of events such as oxidative stress, DNA damage, cell cycle changes and cell death. The latter outcome is why IR …
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Differential proteome analysis of human lung epithelial cells following exposure to aromatic volatile organic compounds
… VOC-mediated molecular effects on human lung cells in vitro at VOC concentrations comparable to exposure scenarios below current occupational limits. Although differential expression of single proteins in response to VOCs has been reported, effects on complex protein networks (proteome) have …
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Investigating WNT signalling in the lung
… of non-canonical WNT signalling, the planar cell polarity (PCP) pathway has emerged as a key ciliary signalling mechanism. Recent studies have highlighted the involvement of core PCP proteins in both ARPKD pathology and lung development and homeostasis. The aim of the study was to determine …
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Compartmental responses of the respiratory tract to Staphylococcus aureus
… at its effects on human primary nasal epithelial cells and their response to TLR ligands. The respiratory tract itself seems to pose a contradiction given by the clinical observation that its upper portion (nasal compartment) allows the growth of bacteria, acting like a reservoir, whereas the …
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Radiation biophysical modeling of the FLASH effect: from mechanistic understanding towards clinical endpoint prediction
… qualities, namely, particle types, energies, and linear energy transfer (LET). In particular, it has been demonstrated using electron LINACs, showed for UHDR irradiation a significant sparing of animals from cognitive deficits in learning and memory. The FLASH effect has been seen using proton …