Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"96 well plate"”.
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Characterization of perillyl alcohol as a cell migration-inhibiting compound
… of the Plopper laboratory designed a 96-well plate, we optimized our in vitro migration and cell viability assays to meet the standards set by the National Cancer Institute for use in a large-scale drug screen for anti-migration compounds. Using these assays, we measured the …
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Fabrication of a Low-Cost, Motorized, 3D-Printed Mechanical Testing Device Compatible with Confocal Microscopy
… Furthermore, with a form factor constrained to a 96-well plate, the device is able to fit into most conventional microscopy stages for simultaneous characterization of microstructure deformation under a quantifiable mechanical load. The construction of this device is performed using …
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Design of the First Analytical Platform for Automated Top Down Proteomics With quadrupole/Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
… dimensional separations platform that utilizes a 96 well plate nanospray robot to facilitate automated analysis on the mass spectrometer. This thesis also highlights instrument advancements required to improve throughput for observation of protein intact masses and methods used to obtain robust …
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Development of A High-Throughput System For Screening of Anti-Prion Molecules
… (i.e., mouse and Syrian hamsters) using a 96-well plate PMCA (96wp-PMCA) platform. Utilizing the 96wp-PMCA technique, a small number of anti-prion and anti-amyloid molecules has been tested against these prion strains using different solvents and at varying concentrations. My results show …
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The Involvement of Progesterone in Glial Cell Viability
… ethynodiol diacetate. Glial cells of 1x10° cells/well were introduced into a 96-well plate and allowed to grow for 24 hours in an incubator at 37°C and 5% CO2. Cells were treated with 0 nM (control), 10 nM, 100 nM, 1 uM, 10 uM ethynodiol diacetate in doublets, with half of the cells being stressed …
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Microfluidic device for cytotoxicity screening
… method requires initial cell seeding in 96-well cell culture plates and a large volume of culturing media, overall a time-consuming and resource-intensive process. We have developed a microfluidic device with internal structures that can evenly distribute cells across five chambers and …
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Use of the Protein Misfolding Cyclic Amplification for food safety and drug discovery
… tested.</p> <p>In parallel, a modified 96-well plate PMCA screening strategy identified eight promising compounds with inhibitory effects on PrP<sup>Sc </sup>formation. Notably, these candidates exhibited diverse properties, including both hydrophilic and hydrophobic profiles, with some …
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Development and evaluation of novel antimicrobial solutions against biofilms and planktonic microbial growth
… system. The Biofilm assays incorporated the MBEC plate, which included use of a 96-well plate with a specially designed pegged lid. Using these assays, the efficacy of the three novel chemical solutions were evaluated as high-level disinfectant, sporicidal, and biofilm disruptor and eradicators. …
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Biochemical Evaluation of SRH Analogs as Potential LuxS Inhibitors
… would be optimized in future to be performed in 96 well plate to avoid excess consumption of enzyme and substrate. In addition, a fluorescence proximity assay is envisioned for the evaluation of a subset of LuxS inhibitors that function by preventing protein dimerization. This assay requires that …
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Development of a high-throughput phenotyping method for measuring sorghum carotenoids
… and UV-VIS spectrophotometric detection in a 96-well plate format was developed and validated. To develop the HTP method, we measured 60 samples using both HPLC and UV-VIS, identifying a strong correlation (R2=0.62, p-value<4.51x10-14) between total carotenoid concentrations measured with the …
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Using a chemical genetics approach to dissect the nitrogen signalling pathway in Arabidopsis
… exposed to a variety of nutrient treatments in a 96-well plate format suitable for chemical genetic screens. This was combined with a newly developed ‘ice capture’ method that provided a rapid and efficient way to harvest root material for the luciferase assay. Using this screening platform in …
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Antiproliferative Activity of Carotenoids, Phenolic Compounds, and Volatile Terpenoids in Carrots (Daucus carota L.)
… respectively. 1000 Caco-2 cells were seeded in a 96-well plate, treated with the carrot carotenoid, phenolic, or volatile extract at a dilution of 50X, 100X, or 200X, then counted at 0, 6, and 12 hours after treatment using the MTS assay. The carrot carotenoids, phenolics, and volatile terpenoids …
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Characterisation and Screening of secondary metabolites from Commiphora viminea (Burtt Davy, Burseraceae) Engl. for antifungal properties against Candida auris
… Antifungal evaluation was performed using a 96-well plate assay filled with 100 μL of Sabouraud Dextrose broth, followed by the addition of 100 of freshly prepared yeast inoculum. Subsequently, 100 μL of crude extracts at a concentration of 5mg/mL was added to each well in the first row and …
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Application of Essential Oil Compounds and Bacteriophage to Control Staphylococcus aureus
… both for treatment of clinical disease as well as in food. Two such antimicrobials with potential application in the food industry include essential oils (EO) and host-specific bacteriophage (phage). The objectives of this study were 1) to determine the efficacy of varying concentrations of …
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Production and characterisation of yeast cell wall preparations with binding activity against Salmonella and Escherichia coli.
… in the cell wall of Saccharomyces cerevisiae as well as other yeast species, MOS have the capacity to inhibit type 1 fimbriae containing pathogenic bacteria from binding to host tissue and thus prevent infection. Alterations in the growing conditions of several yeast strains, including …
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Characterization of Molecular Glycerophospholipids by Quadrupole Time-of-Flight Mass Spectrometry
… of MDCK cells. Finally, combining the use of a 96 well plate and a robotic system demonstrated that these analyses can be automated and analyzed with high throughput. This system we termed Shotgun Lipidomics. Taken together, this mass spectrometric methodology provides rapid and detailed insight …
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Development and application of a high throughput cell based assay to identify apoptosis inducing proteins, and functional characterization of the candidate Vacuole Membrane Protein 1 (Vmp1)
… done using a flow cytometer with an integrated 96-well plate reader. A total of 200 proteins have been screened in the assay, out of which five were identified to be significant activators of apoptosis. One of the candidates, Vacuole Membrane Protein 1 (Vmp1), which forms vacuoles in cells and …
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Tumor cell deformability in the metastatic cascade
… carcinoma cells that had received signaling from platelets to undergo an epithelial-mesenchymal-like transition maintained the same deformability or became less deformable, respectively. In all cases, however, epithelial and mesenchymal tumor cells both take much longer to pass through a …
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Development and application of a high throughput cell based assay to identify novel modulators of ERK1/2 activation and,Functional characterisation of the candidate Radial spokehead like (Rshl1)
… done using a flow cytometer with an integrated 96-well plate reader. A total of 200 proteins were screened, out of which eleven novel cancer relevant modulators of ERK1/2 activation were identified. One of the candidates, the Radial spoke head like -1 (Rshl1), which was identified as an …
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Silica Colloidal Crystals As Emerging Materials For The High-Throughput Sizing Of Proteins By Packed Capillary Electrophoresis
… therapeutic proteins to have high stability as well as high purity. With development time on the order of years and costs incurred in the millions, pharmaceutical companies are investing more time in finding fast and efficient ways to screen for protein heterogeneity. Current methods such as …
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