Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"8-oxoguanine"”.
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Biological response to 8-oxoguanine base released during DNA base excision repair
… A base excision repair pathway initiated by 8-oxoguanine DNA glycosylase (OGG1) excises 8-oxo-Gua lesions from nuclear and mitochondrial DNA, releasing the free oxidized base. It is well-established that failure to repair 8-oxo-Gua can result in mutagenesis and carcinogenesis; however, the …
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Next-generation sequencing as a tool for investigating the in vivo biological consequences of DNA lesions and its applications on the ethenoguanine, 8-oxoguanine and 1,3-butadiene-induced lesions
… simultaneously analyzing the mutagenicity of 8-oxoguanine, a prevalent oxidative lesion, in all 16 adjacent-base sequence contexts. Our result shows that sequence context can significantly modulate 8-oxoguanine mutagenicity. The observed 8-oxoguanine mutational pattern clustered closely with …
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Differences of DNA-Transcription Factor Interactions Following Chromium Exposure
The effect of 8-oxoguanine in the consensus sequence of êB DNA on the binding affinity of p50BD was determined using a 22-mer and a 30-mer double-stranded DNA oligonucleotide containing the NF-êB binding site. The addition of a carboxyl moiety at position 8 on guanine (creating 8-oxoguanine) was …
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Differences of DNA-Transcription Factor Interactions Following Chromium Exposure
The effect of 8-oxoguanine in the consensus sequence of êB DNA on the binding affinity of p50BD was determined using a 22-mer and a 30-mer double-stranded DNA oligonucleotide containing the NF-êB binding site. The addition of a carboxyl moiety at position 8 on guanine (creating 8-oxoguanine) was …
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Consequences of Oxidative DNA Damage: Base-Pair Dissociation, Singlet Oxygenation and DNA-Protein Crosslinking
… investigation of reaction dynamics of 9-methyl-8-oxoguanine (9MOG) radical cations. The experiments of following projects were realized using tandem mass spectrometry coupled with electrospray ionization, followed by the detection of product ions and measurement of cross sections. Potential energy …
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Mechanisms of Oxidative Stress Resistance in Porphyromonas gingivalis W83
… an increase in the oxidative DNA lesion 8-oxoguanine and its related enzymatic removal activity in <em>P. gingivalis</em> FLL92 in comparison with the parent strain. Additionally, a novel removal activity for 8-oxoguanine from duplex DNA was identified in <em>P. gingivalis</em> when …
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Examining the effects of reactive oxygen species on functional potential of HSCS during aging
… are indeed significantly detectable levels of 8-oxoguanine, a lesion associated with ROS, present in both young and old murine HSCs. In an attempt to attenuate the presence of these lesions, a four-week treatment with the thiol-based antioxidant N-Acetyl-L-Cysteine was administered orally to …
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Effect of oxidative stress on human telomeric DNA structural dynamics and accessibility
… (GQ). This guanine-rich sequence is prone to 8-oxoguanine (8OG) damage, which is one of the most common oxidative DNA lesions that can compromise telomere integrity. Here, we use single molecule Förster Resonance Energy Transfer (smFRET) to characterize the effect of 8OG on the structural …
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INFLAMMATION INDUCED CYTOSINE DEAMINATION DAMAGE IN CANCER ETIOLOGY
… mutation due to the oxidation of guanine to 8-oxoguanine that mispairs with adenine during replication. Herein lies the unsolved conundrum- if inflammation causes guanine to thymine transversion mutations, why is the prevalent mutation observed a cytosine to thymine transition mutation? In my …
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Clarifying the Role of the CST Complex in DNA Replication and Repair
… to oxidative DNA damage in the form of 8-oxoguanine caused by exposure to reactive oxygen species and free radicals. These oxidative lesions are repaired by the base-excision repair (BER) pathway. Previous work has implicated telomeric proteins such as the shelterin complex in mediating …
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DNA Repair Mechanisms, Aflatoxin B1-Induced DNA Damage and Carcinogenesis
… damage and carcinogenesis. Mice deficient in 8-oxoguanine glycosylase (OGG1, the rate-limiting enzyme in repair of oxidized guanine), mice heterozygous for OGG1, and wild type mice, were exposed to a single tumourigenic dose (50 mg/kg) of AFB1. Neither ogg1 genotype nor AFB1 treatment affected …
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ARTEMIS AND METNASE MEDIATED PROCESSING OF 3΄-BLOCKED DNA LESIONS: ROLE IN RADIO/CHEMORESISTANCE AND DNA REPAIR
… of commonly found oxidative base damage; 8-oxoguanine (8-oxoG), a hallmark of complex DSBs. However, majority of DNA DSBs are repaired in an Artemis independent manner, and recently discovered, DNA end-specific nuclease, Metnase is a candidate enzyme for repair of such breaks. To study the …
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Interactions of small molecules with duplex DNA and lesion containing G-quadruplex DNA
… the major oxidation product is 7, 8-dihydro-8-oxoguanine (OxodG). OxodG (0.58 V vs. NHE) can further be oxidized in the presence of one electron oxidants and the resulting product forms adducts with endogenous nucleophiles such as spermine. In light of these findings, we hereby designed and …
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Expression and characterization of recombinant human NEIL3 from Escherichia coli
… glycol (TG), 5-hydroxyuracil (5-OHU) or 8-oxoguanine (8-oxoG). Results indicate that the recombinant hNEIL3 proteins are active on single-stranded DNA (ssDNA) substrates containing 5-OHU and TG but show only weak activity on 8-oxoG. In contrast, in double-stranded DNA (dsDNA), activity on …
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Effect of mitochondrial respiratory defecton hepatocellular carcinoma :Involvement of mtOGG1 and NUPR1
… showed decreased expression of mitochondrial 8-oxoguanine DNA glycosylase/lyase (mtOGG1), a mitochondrial DNA repair enzyme; similar results were obtained with human hepatocellular carcinoma tissues. Among several OGG1-2 variants with a mitochondrialtargeting sequence (OGG1-2a, -2b, -2c, -2d, …
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DEVELOPMENT OF HIGH LEVEL AB INITIO METHODS TO DESCRIBE NONADIABATIC EVENTS AND APPLICATIONS TO THE EXCITED STATES OF SMALL BIOLOGICAL MOLECULES
… target system. The excited state dynamics of 8-oxoguanine (8-oG) and its anion are studied in order to assess the possibility of taking part in an electron transfer mechanism to repair a nearby cyclobutane pyrimidine dimer (CPD). It is found that barriers on the anion S1 excited state surface …
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Environmental sensing and response of conserved RNA-processing machines to specific external chemical cues
… that, while one RNA modification of guanine (8-oxoguanine) disables degradation, processing of another modification (1-methylguanine) enhances degradation relative to its unmodified counterpart. The biological implications of selective degradation by this enzyme raises new questions about its …