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Showing 1 to 7 of 7 for “"7-AAD"”.
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Adaptation of Three Different Apoptotic Methods in Equine Bronchoalveolar Cells and Comparison of Bronchoalveolar Lavage Cell Apoptosis in Normal and COPD Affected Horses Before and After Dexamethasone Administration
… Triton-X (PI/Triton-X), 7-aminoactinomycin D (7-AAD), and Annexin V with propidium iodide (Annexin/PI) were evaluated in equine airway lymphocytes. A significant linear relationship was found for equine bronchoalveolar lavage (BAL) lymphocytes stained with 7-AAD and Annexin/PI . No relationship …
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Pesticide Mixtures Induce Immunotoxicity: Potentiation of Apoptosis and Oxidative Stress
… was examined via the 7-aminoactinomycin D (7-AAD) Staining Assay. Apoptosis was detected in each treatment (Mal 6.5%, Lind 12.0%, PBO 13.2%, ML 19.3% and MP 23.4%). Furthermore, 7-AAD staining in combination with fluorescent-labeled monoclonal antibodies, PE-CD45RB/220 and FITC-CD90, was …
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Preclinical Evaluation of Immunomodulatory Effects of Aurora Kinase Inhibition In Human Papillomavirus Positive Cancers
… cell death that was confirmed via annexin V 7-AAD staining as apoptosis. Furthermore, western blot analyses revealed DNA damage, in terms of vii increased levels of γH2AX levels, and pyroptosis, in terms of increased levels of cleaved gasdermin E. Importantly, in both mouse and human HPV+ …
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Immunotoxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and Diethylstilbestrol (DES) in the Fetal Mouse Thymus and Liver
… 18 thymocyte suspensions and flow cytometry, 7-AAD showed decreases in viable thymocytes from TCDD- or DES-treated fetal mice, and concomitant increases in thymocytes in early apoptosis. When thymocytes were co-identified with CD4 and CD8 cell surface antigen expression, enhanced apoptosis …
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Immunotoxic and Oxidative Effects of Endosulfan and Permethrin on Murine SPlenocytes, in vitro
… in combination with 7-Amino Actinomycin D (7-AAD) staining. Endosulfan exposures (25-150 µM) resulted in time- and dose-dependent increase in apoptotic and necrotic cell death in murine splenocytes, in vitro. Permethrin exposure (50-200 µM) resulted in neither a time-dependent/dose-dependent …
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Mechanism-Based Strategies to Enhance The Actions of A
… HSP70. Cytotoxic evaluation (Annexin V/7-AAD assay) of Act D in combination with 17-AAG suggested additive or more than additive interactions. For the second strategy, an agent that affected bioenergy production in addition to targeting transcription and translation was used. Since ATP is …
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The biological effects of HIV-1 Nef on the development of B-cell Lymphoma
… cycle analysis, Annexin V/7-Amino-actinomycin (7-AAD) staining confirmed that this observation was an anomaly, confirming that the Nef protein did not enhance apoptosis in BL cells. Additionally, the Nef protein did not provide any protective effect against apoptosis in BL cells exposed to the …