Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"5mC"”.
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Genetic and biochemical studies of human apobec family of proteins
… AID to deaminate 5-methyl cytosine to thymine (5mC to T). I reexamined this proposed new role of AID (5mC deamination) with two members of the APOBEC family in a novel Escherichia coli based genetic system. My results confirmed that while all three enzymes are strong cytosine deaminases, only …
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The Role of 5-Hydroxymethylcytosine in Genome Instability
… in vivo via the oxidation of 5-methylcytosine (5mC) by members of the Ten Eleven Translocation (TET) dioxygenase enzyme family. Iterative oxidation of 5hmC to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) is also TET-dependent. 5mC represents a critical epigenetic modification, and DNA …
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Tet1: A Unique Dna Demethylase For Maintenance of Dna Methylation Pattern
… the C5 position of cytosine (5-methylcytosine, 5mC) is a crucial epigenetic modification of the genome and has been implicated in numerous cellular processes in mammals, including embryonic development, transcription, X chromosome inactivation, genomic imprinting and chromatin structure. Like …
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Regulation of De Novo and Maintenance Dna Methylation By Dnmt3A and Dnmt3B
<p>DNA methylation (5-methylcytosine, 5mC) is essential for the regulation of gene expression and integrity of the mammalian genome. It occurs predominantly in the context of CpG dinucleotides to form a symmetrical pattern on both DNA strands, which allows DNA methylation patterns to be …
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Chemical and Enzymatic Methods to Detect Chemical Modifications in Nucleic Acids
… its manipulation and detection. In Chapter 3, 5mC pyridination was explored in DNA to determine whether a chemical group could be introduced to enable base resolution detection of 5mC by catalysing a specific 5mC-to-T conversion. In Chapter 4, cyanopyridines containing chemical enrichment …
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Dynamic epigenetic modifications during human fetal germ cell development
… and reductions in 5-methylcytosine (5mC), in the instance of both fetal ovarian and testicular germ cells alike. Furthermore, 5mC was re-detected in human fetal testicular germ cells at fetal stage, but in human fetal ovarian germ cells after birth. The studies in human germ cells …
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Programming of cardiovascular disease: an exploration of epigenetic mechanisms
… subsequent re-establishment of 5-methylcytosine (5mC), however this process has not previously been described for the rat. Furthermore, the involvement of more recently identified cytosine modifications; 5-hydroxymethylcytosine (5hmC), 5- formylcytosine (5fC) and 5-carboxylcytosine (5caC), has not …
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Metabolomics and chemical library screening for antibacterial drug discovery
… cytosine at the C-5 position (5-methylcytosine, 5mC) plays a crucial role in epigenetic transcriptional regulation. TET-family dioxygenases (TET1, TET2 and TET3) initiate active demethylation of 5mC. TET2 oxidizes 5mC into 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and …
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Development Of Single-Cell Transcriptomic & Epigenomic Sequencing Technologies To Assess Cell-Type-Specific Gene Regulatory Programs In Mammalian Brains
… (5hmC)- and “true” 5-methylcytosine (5mC) joint-profiling technology to assess the relationship of two DNA modifications with transcriptional outcomes in the same single cell. The current state-of-the-art DNA methylome profiling technologies rely solely on bisulfite conversion, which …
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Characterization of 5-methylcytosine dioxygenase Tet2 and rescue of mutant Tet2 activity by using turbo co-substrate
… transcription mediated by 5-methylcytosine (5mC) plays a critical role in eukaryotic development. Demethylation of these epigenetic marks is accomplished by sequential oxidation by ten-eleven translocation dioxygenases (TET1-3), followed by thymine-DNA glycosylase-dependent base excision …
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Maintaining Proper Levels of DNA Methylation Marks Through the TET Family is Critical for Normal Embryo Development in Pigs
… catalyzing the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). The three members of TET protein family, TET1, TET2, and TET3, are highly expressed in preimplantation embryos in a stage-specific manner. Previous studies demonstrated that TET proteins are involved in …
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ELUCIDATION OF MECHANISMS GENERATING 5-HYDROXYMETHYLCYTOSINE (5hmC) IN MAMMALIAN MITOCHONDRIA
… to catalyze the oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), an intermediate in the pathway of DNA demethylation. Tet enzymes catalyze this hydroxylation in a 2-oxoglutarate and Fe2+ dependent manner. We have recently reported significant levels of 5mC and 5hmC …
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Decoding 5HMC as an Active Chromatin Mark in the Brain and its Link to Rett Syndrome
… domain (MBD) protein that binds 5hmC and, 5mC, with high affinity. We reveal strong preferential inhibition of MeCP2 affinity to 5hmC by a Rett-syndrome-causing mutation, R133C. We then show that MeCP2 recognizes 5hmC and 5mC within CpA context. Modified CpAs were recently shown to exist in …
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Mechanism and Function of TrkB.T1 Astrocyte Expression
… TrkB.T1. We thus profiled the 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in neurons, astrocytes and microglia utilizing nanopore sequencing. We identified robust differences in cell-type specific TrkB isoform expression is associated with significantly different 5mC and 5hmC …
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Behavioural, genetic and epigenetic determinants of white matter pathology in a new mouse model of chronic cerebral hypoperfusion
… matter regions via perturbations in methylation (5mC) and/ or Ten- eleven translocation proteins (e.g. TET2) in mice. DNA methylation (5mC), hydroxymethylation (5hmC) and TET2 were immunochemically studied in white and grey matter of sham and chronically hypoperfused C57Bl6J mice a month after …
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Functional analysis of 5-hydroxymethylcytosine
… are incapable of distinguishing between 5mC and 5hmC implying the necessity not only to develop techniques specific for 5-hmC characterization but also reevaluation of previously published 5mC data. The biological function of 5-hmC is unknown however many recent studies have suggested a …
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Potential biological embedding of adverse and enriching environments through epigenetic mediators
… mechanisms, such as 5-methyl-cytosine (5mC) marks at cytosine-phosphate-guanine (CpG) base pair sequences, are now regarded as potential mediators of gene by environment interactions. However, current technological limitations make it impossible to measure epigenetics in the living human …
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The role of Ten Eleven Translocation enzymes in the hair follicle mesenchyme
… DNA demethylation by the hydroxylation of 5mC to 5hmC which in turn causes gene transcription activation. Dermal papilla (DP) cells located within the hair follicle are responsible for the regulation of development and the growth of hair follicles. Fgf20 signalling controls commitment of …
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Global alterations of the epigenetic landscape define endometrial receptivity and provide a hallmark for endometriosis
… and H3K27me3, as well as DNA methylation (5mC) and hydroxymethylation (5hmC) profiles by meDIP-seq and hmeDIP-seq, respectively, of endometrial biopsies from control and endometriosis patients. My data reveal considerable changes to the epigenome that occur during the transition from the …
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Evolução cromossômica, disploidia e epigenética no gênero Phaseolus L. (Fabaceae)
… das lisinas 27 e 9, respectivamente) e 5mC (5-metilcitosina) às heterocromáticas. Sítios de DNAr 45S, regiões centroméricas e a maioria dos blocos heterocromáticos terminais foram hipometilados, incluindo um associado a um cluster de genes de resistência à antracnose. Não foi encontrada …
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