Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"5fC"”.
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Chemistry and Biology of DNA-protein cross-links
… characterized and quantified 5-formylcytosine(5fC) mediated DNA-histone conjugates in human cells. 5-Formylcytosine (5fC) is an endogenous DNA modification enzymatically generated in the genome as an oxidation product of 5-methyl-dC (5mC). While 5mC is known to be an epigenetic mark that …
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DNA-Protein Cross-links: Formation, Repair, and Inhibition of DNA replication
… occurring epigenetic marks (5-formyl cytosine, 5fC) in DNA react with lysine and arginine residues of histones H2A, H3, and H4 to form Schiff base conjugates. However, the understanding of cellular effects on DPCs is not fully understood. The main objective of this thesis was to investigate the …
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Metabolomics and chemical library screening for antibacterial drug discovery
… (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by iterative oxidation. Mutations in the TET2 gene are frequently detected in myeloid malignancies. Despite these critical emerging roles of TET dioxygenases in health and diseases, in vitro characterization of …
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Programming of cardiovascular disease: an exploration of epigenetic mechanisms
… (5hmC), 5- formylcytosine (5fC) and 5-carboxylcytosine (5caC), has not been characterised during germ cell ontogeny. Using immunofluorescence to study DNA modifications during late gestation I identified that 5hmC, 5fC and 5caC were present between e14.5 and e16.5 but absent …
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The Role of 5-Hydroxymethylcytosine in Genome Instability
… Iterative oxidation of 5hmC to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) is also TET-dependent. 5mC represents a critical epigenetic modification, and DNA methylation is involved in many biological processes. DNA methylation state is not static; hence, DNA demethylation mechanisms exist …
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Theoretical And Experimental Studies Of Noncovalent Metal Cation Binding And Base Pairing Interactions Of Cytosine And Modified Cytosines
… are 5-methylcytosine (5MeC), 5-fluorocytosine (5FC), 5-bromocytosine (5BrC), 5-iodocytosine (5IC), 1-methylcytosine (1MeC), 5-fluoro-1-methylcytosine (1Me5FC), 5-bromo-1-methylcytosine (1Me5BrC), and 1,5-dimethylcytosine (15dMeC). Relative N3 proton affinities (PAs) of the modified cytosines are …
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Studies on approaches for sequencing cytosine modifications and the identification of their protein readers
Alongside the genetic code, epigenetic mechanisms including covalent cytosine modifications facilitate dynamic changes in gene expression throughout development and differentiation. Such modifications are also drivers and markers of disease, earning them great academic and clinical interest. A …
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Characterization of 5-methylcytosine dioxygenase Tet2 and rescue of mutant Tet2 activity by using turbo co-substrate
… (5hmC), 5-formylcytosine (5fC), and 5-carboxycytosine (5caC) bases in DNA. After 5mCpG, which is the preferred substrate, TET2 prefers 5mCpC as a substrate, followed by 5mCpA and 5mCpT. Because the TDG/BER pathway can convert 5fCpH and 5caCpH to an unmodified cytosine base …