Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"5-hydroxymethyluracil"”.
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PHYSICAL MAPPING OF THE ORIGINS OF REPLICATION OF BACILLUS SUBTILIS BACTERIOPHAGE SP01
… kilobasepairs. It contains the unusual base 5-hydroxymethyluracil in place of thymine. Southern transfer hybridization analysis of pulse-labeled early replicating intermediates was used to localize the origins of replication on the physical map of SP01. The fragments that were consistently …
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Effect of Naturally Occurring DNA Modifications on DNA Structure and Packaging
… indicate that in the sequence context studied, 5-hydroxymethyluracil modifications destabilize dsDNA, while dense symmetrical 5-formyluracil (fU) modifications alter the dsDNA structure. These effects may provide clues to the differential protein recruitment observed in previous research. In vitro …
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High-resolution mapping of abasic sites and pyrimidine modifications in DNA
… the distribution of the modification 5-hydroxymethyluracil at single-nucleotide resolution in the DNA of trypanosomatids. This study provided proof-of-concept of the sequencing methodology in a genomic context and also revealed the genomic features and sequence motifs at which these …
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Methods to Probe the Function of Modified Bases in DNA
This thesis is focused on the development and utilisation of chemical and biological tools to probe the function of modified bases in DNA with specific exploration of the less well-studied T-modifications: 5-hmU, 5-fU and Base J. LCMS/MS techniques are first utilised to enable the accurate global …
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The Role of 5-Hydroxymethylcytosine in Genome Instability
5-hydroxymethylcytosine (5hmC) is a modified base generated in vivo via the oxidation of 5-methylcytosine (5mC) by members of the Ten Eleven Translocation (TET) dioxygenase enzyme family. Iterative oxidation of 5hmC to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) is also TET-dependent. 5mC …