Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"5-azacytidine"”.
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Impact of the DNA methyltransferase inhibitor, 5-azacytidine, on chondrocytic phenotype
… 3) addresses the phenotypic effects of 5-azacytidine (5-aza) in human articular chondrocytes and investigates whether upregulation of collagen type II, observed after 5-aza treatment, is associated with demethylation of the promoter and enhancer CpG island. In this chapter, we also mapped …
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Roles of growth hormone in liver growth and mesenchymal stem cell myogenic and adipogenic lineage commitment
… MSC cell line C3H10T1/2 cells with or without 5'-azacytidine and rbGH for 4 days. We assessed the myogenic or adipogenic potential by determining the ability of these cells to differentiate into myotubes or adipocytes, respectively. C3H10T1/2 cells treated with 5'-azacytidine and GH formed more …
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Dreidimensionales epidermis-ähnliches Wachstum humaner mesenchymaler Stammzellen auf dermalen Äquivalenten : Einfluss der experimentellen Kulturbedingungen auf Differenzierung, Migration und funktionelles Verhalten
… modified by pre-treating the cells with 5-azacytidine or supplementing the medium with all trans retinoic acid. Human MSC were capable of adaptation to epidermis-specific conditions without losing their mesenchymal multipotency. However, despite the viability and evident three-dimensional …
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Studies On The Determinants Of HIV Mutagenesis And Strategies For Its Enhancement
… in the absence of mutational hotspots; 3) 5-azacytidine causes HIV-1 mutagenesis after reduction to 5-aza-2’-deoxycytidine (i.e. decitabine); 4) the anti-HIV-1 activity of 5-azacytidine is antagonized by inhibitors of ribonucleotide reductase; 5) the anti-HIV-1 activity of ribonucleotide …
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Investigating the impact of pharmacological exposures on the developing epigenome
… on imprinted gene regulation in vitro. 5-azacytidine (AZA) and decitabine (DAC) are both cytidine nucleoside analogues used in the treatment of acute myeloid leukaemia. Following incorporation into DNA, both drugs have been demonstrated to induce depletion of DNA methyltransferases …
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The Role of DNA Methylation and Methyl Binding Domain Protein 2 in the Regulation of Human Embryonic and Fetal Beta Type Globin Genes
… mice with the DNA methyltransferase inhibitor, 5-azacytidine, induces a >10-fold increase γ-globin mRNA levels. In addition, βYAC transgenic mice null for methyl binding domain protein 2 (MBD2) express a similar level of γ-globin mRNA. DNA methylation and MBD2 appear to induce γ-globin expression …
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Defining the Transcriptional and Epigenetic Signature of Mouse Embryonic Stem Cells with Compromised Developmental Potency
… Using the DNA methyltransferase inhibitor 5-azacytidine, expression of Gtl2 could be rescued. Injection of those 5-azacytidine treated cells into blastocysts did partially rescue the embryonic lethal phenotype. Additionally, cell surface markers were analysed in a phenotypic screen using …
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Transgenerational Plasticity is Sex-dependent and Persistent in Yellow Moneyflower (Mimulus guttatus)
… seeds with the genome-wide demethylating agent 5-azacytidine erased the effect of maternal but not paternal damage. The number of glandular trichomes remained elevated for three generations following damage. These results indicate that transgenerational transmission occurs through both maternal …
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ROLE OF SPARC IN DORMANCY OF PROSTATE CANCER IN THE BONE
… cells by promoter methylation, but the 5-Azacytidine treatment reactivated the expression. Furthermore, high SPARC promoter methylation negatively correlated with disease-free survival of prostate cancer patients. We also found that the COX2 inhibitor, NS398, downregulated DNMTs and …
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Dissecting the function and targets of FOXG1 in glioblastoma
… de-differentiation, whereas Vitamin C and 5-azacytidine – agents that disrupt DNA and chromatin methylation – can facilitate de-differentiation. CRISPR/Cas genome editing was used to genetically ablate the cell cycle inhibitor FOXO3, or remove the FOXG1-bound cis-regulatory region. These data …
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p53 acts to prevent the formation of pathological R-loops during epigenetic stress
5-Azacytidine (Aza) and 5-Aza-2’-deoxycytidine (Dac) are the two primary therapeutics for the treatment of Acute Myeloid Leukaemia and some myelodysplastic syndromes. The two are frequently used interchangeably, surprisingly as their selectivity of de-methylation is unique from the other, however …
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Epigenetic Modification of Phenotype in Intestinal Smooth Muscle Cells
… were treated with trichostatin A (TSA) and 5-azacytidine (AZA), which inhibit enzymes that deacetylate histones and methylate DNA, respectively, and the outcomes on phenotype were assessed. TSA and AZA treatment decreased the enhanced growth response of repeated passage ISMC, and increased the …
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Single-cell Functional Profiling of Lymphocytes for Cancer Immunotherapy
… therapy of a hypomethylating agent (5-azacytidine) and an immune checkpoint inhibitor (nivolumab, programmed cell death protein 1/PD-1 inhibitor). Sixty-four patient-derived T cells from peripheral blood or bone marrow (site of disease), which were collected before the initiation of the …
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Virus elimination from Actinidia germplasm
… (acyclovir, amantadine hydrochloride, 5-azacytidine, oseltamivir, ribavirin, 2-thiouracil, and vidarabine) against AMV, ASGV, AVX, CLBV-a, and CMV in N. occidentalis shoot tip cultures cultures was determined. Only ribavirin (20mg/L) and ribavirin (10mg/L) combined with quercetin (10mg/L) …
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Hypothalamic mechanisms of appetite regulation involve stress response and epigenetic modification
… (SAM), a methyl donor, and 5-azacytidine (AZA), a methylation inhibitor, to determine their effects on appetite. When measuring food intake immediately post-injection, SAM didn't affect fed or fasted chickens from a line selected for low bodyweight (LWS, individuals with …
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Understanding immune priming in Pacific oysters: A multi-omics exploration of transcriptomic, epigenetic and microbiome regulation
… were exposed to the methylation inhibitor 5-azacytidine at different temporal intervals throughout early development (Chapter 2). A period of methylation inhibitor sensitivity was observed up to approximately 11 hours post fertilisation, which is indicative of a window of methylome …
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Understanding immune priming in Pacific oysters: A multi-omics exploration of transcriptomic, epigenetic and microbiome regulation
… were exposed to the methylation inhibitor 5-azacytidine at different temporal intervals throughout early development (Chapter 2). A period of methylation inhibitor sensitivity was observed up to approximately 11 hours post fertilisation, which is indicative of a window of methylome …
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Downregulation of miRNA expression in malignant germ cell tumours: mechanism and functional significance
… regions. Treatment of the cell lines with 5’-azacytidine resulted in significant upregulation of all three miRNAs (p<0.002), as well as BLID (p<0.02). The methylation status of potential CpG islands at the region of interest on chromosome 11 and chromosome 21 was therefore investigated by …
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DNA Repair Deficiency in Huntington's Disease Fibroblasts and Induced Pluripotent Stem Cells
… expansions, our data reveals that intermittent 5-azacytidine treatments induced <em>HTT</em> gene stability over 4 population doublings, elucidating methylation patterning involvement in TNR instability.</p> <p>Furthermore, induced pluripotent stem cells (iPSCs) undergo global epigenetic changes …
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Diet in the promotion or prevention of colorectal cancer
… silenced by hypermethylation. Using 5-azacytidine, we were able to revert the hypermethylation and induce DNA damage in these cells. We further characterized the model, showing increase in apoptosis, and effects on reexpression of MLH1 protein levels by western blot. We also tested a …
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