Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"5-aza-2’-deoxycytidine"”.
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Studies On The Determinants Of HIV Mutagenesis And Strategies For Its Enhancement
… in the absence of mutational hotspots; 3) 5-azacytidine causes HIV-1 mutagenesis after reduction to 5-aza-2’-deoxycytidine (i.e. decitabine); 4) the anti-HIV-1 activity of 5-azacytidine is antagonized by inhibitors of ribonucleotide reductase; 5) the anti-HIV-1 activity of ribonucleotide …
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Exposures and the Impacts on the Epigenome that Persist from Youth to Adulthood and Intergenerationally
… arsenic and the chemotherapeutic drug 5-aza-2'-deoxycytidine (Decitabine), both of which disrupt different elements of the DNA methylation pathway resulting in persistent epigenetic changes. I first share my findings on the impacts of maternal arsenic exposure, which disrupts the 1-carbon …
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Pathway Profiling Of Replicative And Induced Senescence
… by using various drugs to induce senescence: 5-aza-2-deoxycytidine (a demethylating agent), Adriamycin (a chemotherapeutic drug), and H2O2 (an agent causing oxidative stress).</p> <p>MDAH041 cells, which are fibroblasts isolated from a patient with Li Fraumeni Syndrome, have heterozygous alleles …
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p53 acts to prevent the formation of pathological R-loops during epigenetic stress
5-Azacytidine (Aza) and 5-Aza-2’-deoxycytidine (Dac) are the two primary therapeutics for the treatment of Acute Myeloid Leukaemia and some myelodysplastic syndromes. The two are frequently used interchangeably, surprisingly as their selectivity of de-methylation is unique from the other, however …
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Spectroscopic analysis and synthesis of nucleoside analogs for lethal mutagenesis
… A novel antiviral nucleoside analog, 5-Aza-5,6-dihydro-2'- deoxycytidine (KP1212), employs the mechanism of lethal mutagenesis to avoid the problem of drug resistance and use the virus's high mutation rate against it. KP1212 is incorporated into DNA by HIV reverse transcriptase (RT) but …
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Investigating the molecular dynamics of gene re-silencing following treatment with epigenetic therapies
… in cancer. DNA hypomethylating agents such as 5-Aza-2’deoxycytidine (5-Aza-dC) are approved for the treatment of myelodysplastic syndrome and show promise in the treatment of solid tumours. These therapies are thought to exert their anti-tumour effects by reactivating hypermethylated genes. …
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Functional consequences of cytosine methylation in mitochondrial DNA catalyzed by DNA methyltransferase 1
… inhibition by the nucleoside analog 5-aza-2’-deoxycytidine (5-aza-dC). NRF1 and PGC1α, transcription factors that activate nuclear-encoded mitochondrial proteins in response to oxidative stress, were observed to up-regulate expression of mtDNMT1. Loss of p53, a tumor suppressor gene …
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Aberrant epigenetics in the molecular pathogenesis of human acute myeloid leukemia
… p15 mRNA. Treatment with the cytosine analog 5-Aza-2’-deoxycytidine (5-Aza-dC) in vitro resulted in promoter demethylation and p15 mRNA re-expression, which was associated with a release of a transcriptionally repressive complex at the p15 promoter. Importantly, 5-Aza-dC treatment also reversed …
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Chronic Hypoxia Induces Epigenetic Modifications in the Fetal Rat Heart
… the presence of the DNA methylation inhibitor, 5-aza-2-deoxycytidine, binding of SP1 to PKCε promoter, promoter methylation, and PKCε protein and mRNA were restored to control levels. Connecting epigenetics to chronic hypoxia in utero led us to further investigate the underlining mechanism of …
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Regulation of Matrix Metalloproteinases and Their Inhibitors by DNA Methylation in Human Gestational Tissues
… were treated with the demethylating agent 5-aza-2'-deoxycytidine (AZA) and/or lipopolysaccharide (LPS). qRT-PCR and western blotting revealed synergistic up-regulation of MT1-MMP and TIMP-1, by combined AZA+LPS treatments. Secreted MMP-2 and -9 activities were evaluated by gelatin zymography …
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Zur epigenetischen Inaktivierung der Wnt-Antagonisten SFRP-1, -2, -4 und -5 bei der Akuten Myeloischen Leukämie
… of KG1a and HL60 with the demethylating agent 5-aza-2’deoxycytidine led to a reexpression of SFRP-1 and SFRP-2 in real-time RT-PCR analysis. By MSP, we analyzed the methylation status of SFRP-1,-2,-4 and -5 in 97 specimens obtained from AML patients at diagnosis. The frequencies of aberrant …
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COMBINATORIAL MOLECULAR PHARMACOLOGICAL AND PRECLINICAL STUDIES OF AN EPIGENETIC ANTICANCER DRUG, 3-DEAZANEPLANOCIN A, IN RATS
… through inducing cancer cell apoptosis. 3-deazaneplanocin A (DZNep) appears to be a unique chromatin remodelling (epigenetic) compound that can deplete the cellular enhancer of zeste homolog 2 (EZH2) protein, an enzyme responsible for modulation of histone methylation and in turn specifically …
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A Bayesian Approach to Dose-Response Assessment and Drug-Drug Interaction Analysis: Application to In Vitro Studies
… was declared for DNA methylation inhibitor, 5-AZA-2'-deoxycytidine combined with one histone deacetylation inhibitor, suberoylanilide hydroxamic acid or trichostatin A in the cell lines HEY and SKOV3. This suggests potential new epigenetic therapies in cell growth inhibition of ovarian cancer …
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Glioma genetic profiling: The role of DNA repair and telomerase
… methylation/demethylation in glioma is unknown. 5azadC treatment reduced hTERT expression, which resulted in the downregulation of telomerase protein and its activity. However, an inverse correlation between hTERT and MGMT expression was observed after treatment. Evidence shows that hTERT …
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Identifizierung und Charakterisierung von neuen DNA-Methylierungs-Biomarkern für das humane Mammakarzinom
… in vitro by a DNA demethylating treatment with 5-aza-2’-deoxycytidine and trichostatin A. Therefore, promoter methylation was identified as the major molecular aberration leading to a common expression loss of the analyzed genes in malignant breast cell lines. Next, promoter methylation was …
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The role of Ten Eleven Translocation enzymes in the hair follicle mesenchyme
Epigenetic mechanisms play an important role during the morphogenesis of the hair follicle and the hair cycle. Work on hair regeneration is of importance as no products are available which can provide complete reversal of hair loss. Tet2 promotes DNA demethylation by the hydroxylation of 5mC to …