Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 13 of 13 for “"5-Formylcytosine"”.
-
Studies on approaches for sequencing cytosine modifications and the identification of their protein readers
… Design of an unnatural base pair comprising a 5-formylcytosine derivative with an altered Watson-Crick hydrogen bonding pattern was followed by biophysical confirmation of its pairing in duplex DNA. The unnatural base pair is tolerated by DNA polymerases, allowing optimisation of an expanded …
-
Regulation of nucleosome dynamics
… such as DNA mismatches, 5-methylcytosine and 5-formylcytosine. DNA methylation (5-methylcytosine) decreases DNA flexibility and reduces nucleosome mechanical stability while DNA mismatches and 5-formylcytosine have opposite effects. Our results suggest a completely new mechanism through which …
-
Nucleobase, Nucleoside, And Neighboring Nucleotides: Intrinsic Preferences For Tet Enzyme-Mediated Oxidation Of 5-Methylcytosine
… to 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), and 5-carboxycytosine (caC). The biochemical preference of TET enzymes for these substrates, in the canonical cytosine guanine dinucleotides (CpG), mimics the order in which they are generated and is reflected in levels of these …
-
Effect of Naturally Occurring DNA Modifications on DNA Structure and Packaging
… occupancy and stability revealed that 5-formylcytosine (fC) modifications have positive effects on nucleosome formation and stability compared to the unmodified counterpart by influencing the intrinsic biochemical and biophysical properties of the nucleosomes. These results provide casual …
-
Chemistry and Biology of DNA-protein cross-links
… we discovered, characterized and quantified 5-formylcytosine(5fC) mediated DNA-histone conjugates in human cells. 5-Formylcytosine (5fC) is an endogenous DNA modification enzymatically generated in the genome as an oxidation product of 5-methyl-dC (5mC). While 5mC is known to be an epigenetic …
-
Programming of cardiovascular disease: an exploration of epigenetic mechanisms
… 5-hydroxymethylcytosine (5hmC), 5- formylcytosine (5fC) and 5-carboxylcytosine (5caC), has not been characterised during germ cell ontogeny. Using immunofluorescence to study DNA modifications during late gestation I identified that 5hmC, 5fC and 5caC were present between e14.5 and …
-
The Role of 5-Hydroxymethylcytosine in Genome Instability
… enzyme family. Iterative oxidation of 5hmC to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) is also TET-dependent. 5mC represents a critical epigenetic modification, and DNA methylation is involved in many biological processes. DNA methylation state is not static; hence, DNA demethylation …
-
Tet1: A Unique Dna Demethylase For Maintenance of Dna Methylation Pattern
… 5mC into 5-hydroxymethylcytosine (5hmC), 5-formylcytosine and 5-carboxylcytosine both in vitro and in mammalian cells. Based on their potent oxidative activities on 5mC, TET proteins have shown great potential as the long-sought DNA demethylases that induce DNA demethylation through multiple …
-
Characterization of 5-methylcytosine dioxygenase Tet2 and rescue of mutant Tet2 activity by using turbo co-substrate
… sites produces 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxycytosine (5caC) bases in DNA. After 5mCpG, which is the preferred substrate, TET2 prefers 5mCpC as a substrate, followed by 5mCpA and 5mCpT. Because the TDG/BER pathway can convert 5fCpH and 5caCpH to an unmodified …
-
Methods to Probe the Function of Modified Bases in DNA
This thesis is focused on the development and utilisation of chemical and biological tools to probe the function of modified bases in DNA with specific exploration of the less well-studied T-modifications: 5-hmU, 5-fU and Base J. LCMS/MS techniques are first utilised to enable the accurate global …
-
Metabolomics and chemical library screening for antibacterial drug discovery
… 5mC into 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by iterative oxidation. Mutations in the TET2 gene are frequently detected in myeloid malignancies. Despite these critical emerging roles of TET dioxygenases in health and diseases, in vitro …
-
Characterising the role of TET2 in oestrogen receptor positive breast cancer
… DNA modifications, 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine. These results implied a potential role for GATA3 in modulating reading and writing of DNA modifications as part of the ER complex. In the context of the recently developing focus on TET2 in transcriptional …
-
DNA-Protein Cross-links: Formation, Repair, and Inhibition of DNA replication
DNA-protein crosslinks (DPCs) are ubiquitous DNA lesions that form when cellular proteins become trapped on DNA following exposure to UV light, free radicals, aldehydes, and transition metals. These ultra-bulky lesions are known to disrupt regular DNA cellular machinery, such as replication, …