Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 83 for “"5'-UTR"”.
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Translational Regulation in the 5′UTR of Enteroviruses
The genus Enterovirus consists of a large group of positive-sense RNA viruses, with over 100 serotypes known to be human pathogens. Disease phenotype in humans ranges from sub- clinical to acute flaccid paralysis and treatment options for infection with these viruses are limited, with only few …
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Characterization of 5 UTR splicing and the CGI in HIV-2 RNA
… genomic RNA. Two elements of interest are the 5 UTR intron and the proposed long distance base pairing interaction between the C-box and G-box termed the CGI. This research focuses on the effects of 5 UTR splicing and the CGI in HIV-2 translation and replication. The central hypothesis is that …
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Characterization of 5 UTR splicing and the CGI in HIV-2 RNA
… genomic RNA. Two elements of interest are the 5 UTR intron and the proposed long distance base pairing interaction between the C-box and G-box termed the CGI. This research focuses on the effects of 5 UTR splicing and the CGI in HIV-2 translation and replication. The central hypothesis is that …
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Analysis of cis-acting expression determinants of the tobacco psbA 5’UTR in vivo
… to be regulated via its 5’- untranslated region (UTR). Some cis-acting elements within this 5’UTR and the correlated trans-acting factors have been defined in Chlamydomonas. However, no in vivo evidence with respect to the cis-acting elements of the psbA 5’UTR has been so far achieved in higher …
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Zur Bedeutung der polyTC-Motive in den Promotoren und 5‘-UTR-Bereichen konstitutiv exprimierter Pflanzengene
… (Beta vulgaris) besitzt im Promotor und der 5’UTR je eine Polypyrimidinbox. Solche Motive aus sich häufenden TC-Alternierungen sind in pflanzlichen Promotoren und 5’UTRs ein häufig auftretendes Motiv mit bislang weitgehend ungeklärter Funktion. Am Beispiel des Promotors Y11037 des konstitutiv …
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Direct Recruitment of eIF4GI and/DAP5 to the 5' UTR of a Subset of Human mRNA Drives Their Cap-independent Translation
… (e.g., tumor hypoxia, viral infection, nutrient deprivation, etc.), the canonical, cap-dependent translation initiation pathway in human cells is suppressed by sequestration of the cap-binding protein, eukaryotic initiation factor(eIF) 4E, by 4E-binding proteins. Circumvention of …
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GCD10 and GCD14 Proteins Facilitate Tomato Bushy Stunt Viral (TBSV) RNA Replication in Saccharomyces Cerevisiae
… studies showed that the 5 untranslated region (5UTR) of the genome is important, albeit not essential, for viral RNA replication in plants. Here, my study shows that the 5UTR is also important for viral RNA replication in S. cerevisiae, a model host for TBSV. Using the TBSV 5UTR as bait RNA and a …
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The Role of Secondary and Tertiary Structure in the Cap-Independent Translation of FGF-9 and HIF-1-alpha
… directly to the 5’ untranslated regions (5’ UTR) of the RNA. eIF4G binding then recruits the ribosome to the transcript. While this mechanism is useful for translation of apoptotic transcripts and transcripts involved in cell survival, cap-independent translation is also utilized by oncogenic …
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Characterization of the 5̕ untranslated region ( 5̕ UTR) of the alcohol oxidase I (AOX I) gene in Pichia pastoris.
… of the 122 nucleotide 5' Untranslated Region (UTR) of the Alcohol Oxidase I (AOXI) gene in Pichia pastoris. The 5' UTR influences the expression of many heterologous proteins in P. pastoris. However, no systematic analysis has ever been performed on this region to date. Several truncated …
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Strucutre-function studies of the venezuelan equine encephalitis virus 5'UTR promoter element and its role in attenuation of the virus
… encoded by the 5’ \r\nUntranslated Region (5’UTR), in regulating virus replication and attenuation of \r\nVenezuelan equine encephalitis virus (VEEV) is described. VEEV is one of the \r\npathogenic members of the Alphavirus genus in the Togaviridae family. VEEV \r\ninfection causes …
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Mechanisms of Regulation of Tau Ires Mediated Translation
… manner by utilizing sequences in their 5’ UTR called IRES. It was previously shown that the 5’ UTR of the tau mRNA contains an IRES. In this study I show that IRES dependent translation of tau IRES is regulated at multiple levels in order to regulate the expression of the tau protein.</p> …
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Mapping RNA Binding Surfaces on Hfq Using Tryptophan Fluorescence Quenching
… Two identified binding sites located in the 5'UTR of hfq mRNA, site A and site B, were used for TFQ, along with a longer RNA sequence that contains both sites and their native linker, 5' UTR. TFQ illustrates that the individual sites and the 5' UTR are capable of binding both faces. Each site …
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STUDIES OF THE DIMERIZATION AND PACKAGING SIGNALS IN HIV-2 RNA
… in the 5' untranslated leader region (5'UTR) of HIV genomic RNA is essential for understanding viral replication because the 5'UTR regulates several essential functions by the alternate presentation and sequestration of signals through conformational changes. Our main focus in this study …
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STUDIES OF THE DIMERIZATION AND PACKAGING SIGNALS IN HIV-2 RNA
… in the 5' untranslated leader region (5'UTR) of HIV genomic RNA is essential for understanding viral replication because the 5'UTR regulates several essential functions by the alternate presentation and sequestration of signals through conformational changes. Our main focus in this study …
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Nucleic acid clamp-mediated transcriptional and translational modulation of oncogenes via the stabilization of G-quadruplexes
… the promoters of DNA, 5’-Untranslated regions (UTR) of mRNA and telomeres. These secondary structures tend to cluster around 1 kb from transcriptional start sites across all human chromosomes in addition to at telomeric regions. Extensive efforts from many research groups have been dedicated to …
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Integration of small RNAs in the control of Salmonella pathogenicity island 1(SPI1)
… PinT also directly interacts with 5’ UTR of rtsA transcript to cause both translational inhibition and degradation of the rts transcript. PinT regulates flhD expression by repressing crp expression, resulting in downregulation of HilD protein activity through FliZ. In addition to the …
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A Novel HIV-1 Genomic RNA Packaging Element and its Role in Interplay between RNAPackaging and Gag-Pol Ribosomal Frameshifting
… such as HIV-1 to the 5’untraslated region (UTR) containing four hairpin structures. However, most of these studies only focused on small fragments of the 5’UTR and therefore the evidences were not sufficient to consider the ¿ as the sole packaging element. A central goal of this dissertation …
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Understanding small RNA-mediated post-transcriptional regulation of Salmonella pathogenicity island 1 activator HilA in Salmonella typhimurium
… proteins. The hilA mRNA 5' untranslated region (UTR) is ~350 nucleotides in length and binds the RNA chaperone Hfq, suggesting it is a likely target for sRNA-mediated regulation. I compared two high-throughput approaches for sRNA target identification. I developed a modified MS2-Affinity …
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MicroRNA-122 and Poly(C)-Binding Protein 2 Bind to the Hepatitis C Virus Genome, Regulating Viral Replication and Genome Structure
… binding sites on the 5’ untranslated region (UTR) of the HCV genome. This work investigates how miR-122 and PCBP2 bind the 5’ UTR of the HCV genome, and how the competition between the two factors regulates viral translation and replication. The binding competition of miR-122 and PCBP2 was …
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Engineered mRNA regulation using an inducible protein-RNA aptamer interaction
… RNA elements into the 5'-untranslated region (5'-UTR) of a given mRNA, translation of a downstream coding sequence is directly controlled by TetR and tetracycline analogs. In endogenous and synthetic 5'-UTR contexts, this modular system efficiently regulates the expression of multiple target …
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