Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"4T1"”.
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A bélflóra megváltozásának hatása 4T1 emlőtumoros egerek túlélésére
… kísérleteink igazolták, hogy a LCA csökkenti a 4T1 emlő tumor sejtek proliferációs- és áttétképző képességét. Mivel a LCA-at a szervezetben kizárólag a bélflóra baktériumai állítják elő, olyan kísérletet terveztünk, melyben a bélflóra megváltozásának hatását 4T1 vizsgáltuk emlőtumoros egerek …
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Astaxanthin Decreases Invasion and MMP Activity in Triple Negative Breast Cancer Cells
… that Astaxanthin decreases invasion of 4T1 cells through Matrigel<sup>TM</sup>, but does not affect proliferation. Using zymography we showed that 4T1 cells treated with Astaxanthin demonstrate decreased MMP-2, -9, and -13 activities. Western blot analysis of conditioned media and …
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Delineating the role of prolactin-inducible protein (Pip) in breast cancer lung metastasis
… transplantable mouse model of BC utilizing the 4T1-mouse metastatic BC cell line and determined that while over-expression of mouse homologous (Pip) suppressed tumor development in the primary tumor in the breast, it enhanced metastasis in lungs. We hypothesized that in the lung, Pip promotes a …
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Metabolic-response assessment of metastatic murine breast cancer in 2D and 3D cultures using intrinsic NADH as a natural biomarker
… (NADH) in metastatic murine breast cancer cells 4T1. In addition, we investigated the cellular metabolic response of 4T1 cells in 2D monolayer and 3D collagen matrix cultures to drug treatment using two novel metabolic drugs, namely MD1 and TPPBr. Both 2P-FLIM and complementary time-resolved …
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Development and Characterization of an Autologous Whole Cell Breast Cancer Vaccine
… cancer vaccine using two murine cell lines, 4T1 and EMT6, to demonstrate the potential of vaccines for adjuvant treatment of breast cancer. We first tested two commonly used methods of inactivating cells, irradiation and freeze/thaw cycling, to see if either method was superior in …
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Role of Jak/Stat pathway in the pathogenesis of breast cancer
… of breast cancer. For these studies, we used 4T1 mouse mammary tumor cells as a model which mimics human breast cancer. First, we investigated the role of Tyk2 tyrosine kinase in the pathogenesis of breast cancer. Here we show for the first time that compared with wild type mice, Tyk2 -/- mice …
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Rerouting Pre-Existing Host Vaccine-Induced Immunity to wards Breast Cancer
… enhanced MHC-I expression <em>in vivo</em> in 4T1 mouse mammary tumors engineered to overexpress human HER2. To test our hypothesis of therapeutically redirecting preexisting non-cancer immunity developed through vaccination or contract with an infectious disease to cancer in vivo, we first …
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Platinum-NGR A Peptide Targeting Conjugate Of Cisplatin: Design, Synthesis, And Efficacy In Breast Cancer Cells
… was then employed in in vitro testing in 4T1 breast cancer cell lines that are CD13 positive. Cell viability, drug competition, and drug uptake were assessed in 4T1 cells for cyclic mPeg-CNGRC-Pt versus carboplatin and free peptide. Our results suggest that cyclic mPeg-CNGRC-Pt has …
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Identification and Knockout of Immunodominant Endogenous Retroviral Antigen in Murine Tumor Models
… used for preclinical studies B16F10, MC38, 4T1, and CT26 were able to produce replication-competent eMLV able to infect other murine cells in vitro and T cells in vivo. Human tumors have not been shown to produce infectious ERV, and so we removed the infectious potential of these murine …
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The Role of Inflammation and Nitric Oxide Synthase 2 in Triple-Negative Breast Cancer Metastasis
… knockout of the NOS2 gene in the 4T1 parental cell line significantly suppresses lung metastasis from primary 4T1 tumors in Balb/c mice, providing direct evidence that inhibition of NOS2 in tumor cells can suppress metastatic progression in TNBC. We used flow cytometry to immune …
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Dissecting cell-autonomous and microenvironmental factors governing lymphatic metastasis in breast cancer
… Using transplantable syngeneic mammary carcinoma 4T1 model, a differential preference for using the lymphatic system as a dissemination route was observed in immunocompetent mice when compared to immunodeficient mice. Characterisation of the differences in the tumour microenvironment revealed a …
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Changes of breast cancer growth and metastasis in murine models modulated by an aromatase inhibitor, a low calcium diet or a high fat diet
… lung metastases in mice inoculated with murine 4T1 cancer cells. In a following project, the effects of a Low Calcium Diet (LCD) on BC metastases from bone to lungs, and its effects on the bone microenvironment in mice inoculated with murine 4T1 cells were studied. Bioluminescence imaging and …
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Characterizing the role of Nucleosome Remodeling Factor (NURF) in tumorigenesis and metastatic progression using mouse models of breast cancer.
… knocked down mouse breast cancer cell lines, 4T1, 66cl4, and 67NR, into syngenic BALB/c mice. Our findings reveal decreased tumor growth in 66cl4 and 67NR as measured by tumor weight at 3-4 weeks post injection. Tumor growth did not appear to be significantly affected in 4T1 challenged mice. …
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Metaboloptics: In Vivo Optical Imaging to Enable Simultaneous Measurement of Glucose Uptake, Mitochondrial Membrane Potential, and Vascular Features in Cancer
… comparing in vivo glucose uptake of metastatic 4T1 and nonmetastatic 4T07 murine mammary adenocarcinomas. Further, the addition of vascular information revealed metabolic heterogeneity within the tumors. The primary conclusion of Aim 1 was that delivery-corrected 2-NBDG uptake (2-NBDG60/RD) is …
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The Treatment of Solid Tumours with Complete Freund's Adjuvant
… survival in both the P815 mastocytoma and 4T1 mammary cancer models. CFA treatment was not, however, found to be effective in the CT26 colorectal carcinoma and the DEN-induced Hepatocellular carcinoma. Combination therapy with CFA and anti-CTLA-4, which blocks the inhibitory effects of …
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The Treatment of Solid Tumours with Complete Freund's Adjuvant
… survival in both the P815 mastocytoma and 4T1 mammary cancer models. CFA treatment was not, however, found to be effective in the CT26 colorectal carcinoma and the DEN-induced Hepatocellular carcinoma. Combination therapy with CFA and anti-CTLA-4, which blocks the inhibitory effects of …
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Potentiation of The Immune Checkpoint Blockade Response By Metabolic Modulation Is Predictable Using Molecular Imaging
… Resonance Imaging (acidoCEST MRI), pHe of 4T1 tumors after esomeprazole can predict response to combination therapy, as indicated by increased T cell activation. However, the change in B16-F10 pHe after esomeprazole predicts response to combination therapy, whose increase is compensated by …
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The effects of thermally abused frying oil polar fraction on breast cancer motility and proliferation in vitro
… cells in vitro, including the impact on 4T1 cell migration and proliferation. Wound healing and transwell migration assays were conducted to assess the effects of TAFO-PF on cell migration and proliferation assays were used to measure proliferation. The major findings in this thesis were: …
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Delivery of Myoglobin Polymersomes Results in Tumor Hemorrhagic Necrosis and Enhanced Radiation Response
… polymersome accumulation in orthotopic 4T1 mammary carcinomas. In addition, a novel method for measuring pharmacokinetics was developed, using serial small volume blood draws from individual mice. The plasma fluorescence in microcapillary tubes was used to quantify polymersome …
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An Exploration of the Feasibility of Combining Radiation Therapy with Psoralen Phototherapy
… was observed in various murine cancer cells (4T1, KP-B, and CT2A) when treated with a combination of 50µg/mL phosphor, 10µM psoralen (8-MOP), and 1Gy of 80kVp x-ray (viability < 20%), compared to any of these components alone (viability > 70%). This suggests a synergistic interaction between …
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