Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"4E-BP1"”.
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Mechanism-Based Strategies to Enhance The Actions of A
… status of the translation repressor 4E-BP1, whose activity is modulated by upstream kinases sensitive to changes in ATP levels, were also performed. The hypophosphorylated state of 4E-BP1 leads to translation repression. Data indicated that treatment with 17-AAG alone resulted in a …
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Examination of type I IFN mediated regulation of the post-transcriptional antiviral response
… lacking suppressors of translation, TSC2 or 4E-BP1, are more sensitive to IFN-α/β inducible effects as indicated by increased antiviral protein synthesis, and enhanced resistance to virus infection. In vivo experiments showed that mice lacking 4E-BP1 are similarly more resistant to virus …
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Defining Determinants of Primary Drug Resistance in Precision Cancer Therapies
… therapy drives cell death through the 4E-BP1-dependent suppression of the translation and consequent stability of driver oncoproteins, including MYC. Further, with mechanistic intent, using a genome-wide CRISPR/Cas9 screen, we identified the CCR4-NOT complex as a major determinant of …
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Long-term activity-induced changes in the brain : a study of translational regulation and structural plasticity
… of the translation factors S6, elF4E and 4E-BP1. Increased phosphorylation of translation factors was observed along with increased translation after the induction of L-LTP; lastly, LTM formation occurred concomitantly with ERK MAP Kinase pathway-dependent phosphorylation of S6 and …
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Genetic Analysis of the PI3K/AKT/mTOR Signaling Pathway
… PI3K/AKT/mTOR pathway members: AKT1, p70S6K and 4E-BP1) in a variety of contexts, including under baseline cell growth conditions and in response to treatment with different PI3K/AKT/mTOR pathway inhibitors. Traditional genetic analyses were used to identify pathway activation phenotypes that …
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Protein quality and distribution determines anabolic signaling, cellular energetics, and body composition
… involves phosphorylation of the inhibitory eIF4E binding protein-1 (4E-BP1), which releases the initiation factor eIF4E and allows it to bind with eIF4G. Binding of eIF4E with eIF4G promotes preparation of the mRNA for binding to the 43S pre-initiation complex. Consumption of the amino acid …
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Investigation into the mechanisms responsible for muscle atrophy in cancer cachexia
… initiation factors through dephosphorylation of 4E-BP1 and an increase eEF2 phosphorylation was seen in response to PIF in vitro. The same pattern of events also occurred in gastrocnemius muscle of mice bearing the MAC16 tumour demonstrating weight loss, where a depression of mTOR and p70S6K …
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mTOR Signaling in Cellular Growth and Skeletal Muscle Maturation
… translation through the phosphorylation of 4E-BP1 and S6K. Here, I investigated the function of mTOR for the activation of newly found S6K1 homologue, S6K2, and for the regulation of skeletal muscle myotube growth. My results showed that S6K2 requires mTOR and its kinase activity for its …
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The Effect of Post Exercise Nutrition on Anabolic Response to Resistance Exercise
… exercise bout increased the amount of eIF4E-eIF4G by 4.5% 1-hr postexercise (p<0.05) without affecting the amount of eIF4E-4E-BP1. One hour after beverage consumption, serum total amino acid concentration increased for MILK 1 (p=0.003) and MILK 2 (p<0.001) but decreased for CE (p=0.028) …
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Investigating metabolic and mitochondrial adaptations triggered by blood flow during the endothelial-to-hematopoietic transition
… signaling regulates mTORC1 effectors S6K and 4E-BP1 to promote translation of mitochondrial ribosomes and electron transport chain proteins.</p> <p>Our work exposes an overlooked role of force in the maturation of mitochondrial machinery essential for HSC emergence and population of the blood …
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AMP-activated protein kinase-mTOR signaling pathway and hepatitis B virus replication
… eukaryotic translation initiation factor 4E-binding protein 1(4E-BP1)의 발현과 인산화는 HepG2.2.15세포에서 증가하였다. Huh7 HBV WT stable 세포에서는 S6K1의 인산화, PP2A의 발현과 인산화가 증가하였다. AMPK alpha 2가 과발현 될 때 HBV의 core particle과 그 속의 핵산, HBV의 DNA가 감소하였다. HepG2.2.15 세포에 metformin을 처리하였을 때 AMPK의 인산화는 증가하였고 이에 따라Akt, …
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Insights into the regulation of mTOR signaling and the consequences of pharmacological inhibition
… machinery through its substrates S6K and 4E-BP1. Additionally, S6K can suppress insulin signaling, establishing a negative feedback loop to PI3K. Consistent with its role in cell growth, derangements in mTOR signaling are increasingly associated with cancer and, more surprisingly, …
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Elucidating the Roles of Eukaryotic Initiation Factors Involved in DAP5 Mediated Translation
… which consists of the cap binding protein eIF4E, the scaffolding protein eIF4GI, and the helicase factor eIF4A.The recognition and binding of eIF4E to the m<sup>7</sup>G cap structure of the mRNA is essential for the subsequent recruitment of eIF4GI and eIF4A to form a translationally active …
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Regulation of mTOR complex 1 in response to growth factors and nutrients
… translation through its substrates S6K and 4E-BP1. Many of the upstream signals that lead to activation of TOR pathway are tumor suppressors and deregulation of the pathway leads to disease. We aim to better understand the regulation of mammalian TORC1 (mTORC1) by upstream signals. In the …
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A mechanistic examination of the metabolic regulation of insulin resistance, sarcopenia, and osteoporosis
… or total and phosphorylated Akt, mTOR, S6K1 or 4E-BP1 response. The magnitude of the anabolic response to an oral EAA ingestion in healthy older men and women remains intact after 3 months of EAA supplementation.\r\nStudy 3) Older men received continuous weekly testosterone injections for 5 …
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Regulation of PPARgamma Activity by mTOR in Adipogenesis
… nuclear shuttling of mTOR and S6K1 activation/4E-BP1 phosphorylation. Furthermore, it was found that nuclear shuttling of mTOR regulates mitogen-stimulated rapamycin-sensitive translation initiation. These findings uncover a function for the nucleus in the direct regulation of the protein …
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Pim Kinase Inhibition: Single Agent and Combination Approaches In B-Cell Lymphoma
… and translation regulators, c-Myc and 4E-BP1 declined in both model systems. Additionally, levels of short-lived Mcl-1 mRNA and protein also decreased and correlated with decline of global RNA synthesis. Collectively, our investigations highlight Pim kinases as viable drug targets in …
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α7β1 integrin regulation of skeletal muscle growth in response to mechanical stimulation
… protein (YAP) content, and increased phospho- 4E-BP1 on Ser65. In Aim 3, α7Tg overexpression was not sufficient to rescue the anabolic response to chronic loading in aged mice, yet injection of LM-111 significantly restored integrin localization at the membrane and the anabolic response to a …
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Mechanistic insight into the role of dietary fibers and short chain fatty acids in the regulation of metabolism and inflammation in the pig
… restored LPS-induced reduction in Akt and 4E-BP1 expression levels, accounting for its effect in enhancing tight junction intergrity. In addition, butyrate also acted as energy source fueling epithelial cell recovery, evidenced by the changes in AMPK abundance and intracellular ATP level. …
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Regulation of mTORC1 Signalling and Amino Acid Uptake by Iron Availability in Caco-2 Intestinal Epithelial Cells
… of ribosomal S6 protein and that of 4E-BP1 phosphoryation on Ser65, all of which serve as positive downstream readouts of mTORC1 signalling. The loss in phosphorylation of these mTORC1 targets was reversed upon cellular resupplementation of iron, treatment with Actinomycin D (which …
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