Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"4CL"”.
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Improving barley for biofuel production – investigating the role of 4CL and CCR in the lignin biosynthesis pathway
… bioenergy crop itself. This thesis concerns the 4CL and the CCR enzymes on the lignin pathway which were chosen as the targets to manipulate lignin in barley. They were selected because there is evidence that suppression of each of them in dicot species can lead to increased saccharification. The …
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Molekulare, physiologische und biochemische Charakterisierung der Genfamilie für 4-Cumarat:Coenzym A Ligase aus Sojabohne (Glycine max L.)
Die 4-Cumarat:Coenzym A Ligase (4CL) ist ein Enzym des allgemeinen Phenylpropanstoffwechsels, das aktivierte Hydroxyzimtsäure-Ester zur Synthese spezifischer Phenylpropanoide bereitstellt. In vielen Pflanzen kommen strukturell und funktionell sehr ähnliche 4CL-Isoformen vor, weshalb diesem Enzym …
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Genetic Improvement of Switchgrass Cell Wall Content, Leaf Angle and Flowering Time
… production. 4-Coumarate: Coenzyme A ligase (4CL) is one of the key enzymes involved in the monolignol biosynthetic pathway. Two homologous 4CL genes, Pv4CL1 and Pv4CL2, were identified in switchgrass. Gene expression patterns and enzymatic activity assays suggested that Pv4CL1 is involved in …
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Structural studies on the biosynthesis and biological functions of natural products
… development. Studies on 4-coumarate:CoA ligase (4CL), the enzyme that synthesizes 4-coumarate-CoA, are thus crucial for plant engineering and drug discovery. In Chapter 3, I present thorough biochemical and structural studies on a tobacco 4CL enzyme, and a successful example of engineering this …
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Fungal Degradation Properties of Young Small Diameter Genetically Modified Quaking Aspen (Populus tremuloides)
… lignin content through transfer of an antisense -4CL gene, changed syringly/guaiacyl ratio through insertion of a sense CAld5H gene, and modified lignin content and syringyl/guaiacyl through simultaneous insertion of -4CL and CAld5H genes. Mass loss was used to examine differences between genetic …
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Paralogous genes in Arabidopsis thaliana contribute to diversified phenylpropanoid metabolism
… 4-hydroxylase (C4H) and 4-coumarate: CoA ligase (4CL). Cinnamoyl CoA reductase (CCR) then converts p-coumaryl CoA to p-coumaraldehyde, the substrate of CYP84A4. Through the analyses of mutants that are defective in these genes, stable-isotope labeling studies, and chemical complementation …
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Characterisation of chromatography adsorbents for antibody bioprocessing
… Ultra (Millipore), Protein A immobilised on 4CL Sepharose (GE Heatlhcare) in house and a prototype adsorbent with a Protein A mimic ligand (Millipore). Both down-scaled techniques of fixed bed chromatography, together with supporting analysis of equilibrium and dynamic behaviours are used. …
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Identifying and characterizing genes and transcription factors involved in coloration of apple fruit
… (C4H) and 4-coumaroyl:CoA-ligase (4CL) genes, both important in lignin biosynthesis, and possibly contributing to modulation of floral morphogenesis. Moreover, transgenic flowers had longer styles than those of wild-type flowers, suggesting that the MdMYB11 gene might be involved in …
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Synthesis, characterization, and anticancer activity of arene mono-ruthenium and heteroleptic mixed-valent diruthenium complexes
… the precursor tetraacetate complex [Ru2(O2CCH3)4Cl] by one substituted ap ligand. This afforded stable mixed-valent diruthenium(II, III) paddlewheel complexes with a general chemical formula [Ru2(O2CCH3)3(R-ap)Cl]. Complexes [Ru2(O2CCH3)3(4-CH3ap)Cl] (C3) and [Ru2(O2CCH3)3(4-Fap)Cl] (C8) show …
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Secretomotor substances in the rat lacrimal gland: Immunocytochemical functional and ligand binding studies
… was abolished by the VIP receptor antagonist [4Cl-D-Phe 6, leu' 7} VIP, which reduced the response to NPY. In addition, when VIP was combined with NPY an attenuated response was observed. VIP and NPY binding sites were present on lacrimal acinar cells, but binding sites for NPY were far less …
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Blei(II)- und Zinn(II)-Komplexe mit Polyolen und a-Hydroxycarbonsäuren sowie Blei(II)-Cyclodextrin-Einlagerungskomplexe
… aufgebaut. Der Cluster Sn6(OMe)3(O)4Cl (22) kann als ein Zwischenprodukt der Hydrolyse von Dimethoxy-Zinn(II) angesehen werden. Er besteht aus einem adamantanartigen Sn6O4-Gerüst sowie dreifach verbrückenden Methoxygruppen. Bei Einbeziehung des freien Elektronenpaars ergibt sich …