Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"454-pyrosequencing"”.
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COMMUNITY ANALYSIS OF A UNIQUE FULL-SCALE WASTEWATER TREATMENT PLANT AS REVEALED BY 454-PYROSEQUENCING
… treatment plant that may benefit from the use of pyrosequencing techniques is the Los Alamos National Laboratory Sanitary Wastewater System (LANL SWWS) located in Los Alamos, New Mexico. This plant experiences highly variable flow and utilizes glycerin as a supplemental carbon source due to …
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The impact of chlortetracycline on Drosophila melanogaster and Aedes aegypti
… 50 µg ml-1 and above. Culturable techniques and 454 pyrosequencing, demonstrated that the microbial diversity of Drosophila was predominantly Acetobacter. Bacterial elimination through egg dechorionation yielded some similar results to chlortetracycline treatment. However, fecundity and life-span …
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Diversity and spatial distribution of fungal endophytes in a Eucalyptus grandis tree
… bar-coding, or metagenetics, was done using 454-pyrosequencing parallel sequencing of the barcode amplicons of all the fungal isolates present in the plants from the total DNA of the plant. Secondly, conventional DNA bar-coding of was done of fungal endophyte isolates. Isolates of the …
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Domestic 'laker' ships as a potential pathway of nonindigenous species in the Great Lakes
… ports, and identified invertebrates through 454 pyrosequencing of 18S rDNA. Ballast samples contained 12 brackish potential nonindigenous species, while St. Lawrence River port samples contained two fresh and 27 brackish species. Québec City poses the greatest establishment risk due to high …
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Exploring the Potential Use of Camelina Sativa as a Biofuel Crop for Nevada
… analysis of C. sativa was performed using 454 pyrosequencing to characterize expressed genes in roots, stems, leaves, flowers, and developing seeds of mature plants subjected to drought, cold and high salinity stress Some contig assemblies corresponding to stress-inducible gene and genes …
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Data Management tools For Metagenomic Datasets, A Case Study: Characterization and Dysbiosis of Gut Microbiota In Apcmin/+ Mice With and Without Colon Tumors
… and storing metagenomic sequences obtained from 454-pyrosequencing technology. </p> <p> The data management pipeline was optimized using microbial metagenomes isolated from colon tissue and stool samples of APCmin/+ mice (a mouse model of colon cancer). A total of 171,405 unprocessed sequences …
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Identifying ecological differentiation in microbial communities across taxonomic scales
… whole-ecosystem disturbance along with 16S rRNA 454-pyrosequencing to identify ecologically relevant distinctions among taxonomic groups defined at various taxonomic scales. The findings showed that bacterial lineages require different taxonomic definitions to capture ecological patterns. At a …
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Genome composition of Glycine max and sequence diversity among cultivated and exotic accessions
… content and diversity. The first study applied 454 pyrosequencing to obtain a low coverage survey that identifi ed repeat composition of the genome. The second study compiled data from numerous small RNA sequence datasets to follow the small RNA level regulation of soybean genes and the …
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Bacterial Degradation of Isoprene in the Terrestrial Environment
… isotope probing experiments combined with 454 pyrosequencing.
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Population genetics of the farmland sawfly <em>Dolerus aeneus</em> (Hymenoptera, Symphyta)
… sawfly transcriptomic library through the use of 454 pyrosequencing, the first genetic resource for any farmland sawfly. From this library, a set of 13 polymorphic microsatellite markers were isolated for use in the common farmland sawfly Dolerus aeneus. Using these markers, three Scottish …
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Assessment of the immunomodulatory effects of raw/farm milk and probiotic bacteria
… using next generation sequencing technology (454 pyrosequencing). Here the presence of previously unrecognised and diverse bacterial populations in unpasteurised cow’s milk was identified. Futhermore the bacterial content of pasteurised milk was found to be more diverse than previously …
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Antimicrobial activities and diversity of sponge derived microbes
… and archaeal diversity by application of 454 Pyrosequencing. The L. diversichela –proteobacterial community was dominated by a single ƴ-proteobacterial bacterium whereas the S. normani sample hosted a largely sponge specific microbial community, even more diverse than has been previously …
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Analysis of microbes in Greenland ice cores from periods of high and low atmospheric carbon dioxide levels
… meltwater were subjected to 454 pyrosequencing. Taxonomic analyses were based on rRNA and mRNA sequences. Taxa in the 10,500 ybp ice primarily included bacterial species of Proteobacteria, Firmicutes, and Cyanobacteria. This sample also contained black particulate matter …
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Bacterially-Mediated Formation of Rock Coatings in Kärkevagge, Swedish Lapland: A Mineralogical and Micro-Environmental Analog for Mars
… diversity surveys - Sanger sequencing and 454 pyrosequencing. Using the results of these surveys, we were able to assess diversity, richness, and metabolic potential of the communities. Microscopy and spectroscopy were used in order to visualize microbial communities inhabiting the coatings …
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Characterization of the prokaryotic community associated with the giant barrel sponge, Xestospongia muta across the Caribbean
… across the Caribbean were quantified using 454 pyrosequencing of the 16S rRNA gene. Phlyogenetic analysis showed differences between the sponge prokaryotic community and the surrounding bacterioplankton. Additionally, both symbiont and bacterioplankton populations were different between …
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Intersexuality and Endocrine Disruption in the Amphipod Echinogammarus marinus – From Genes to Physiology
… gonadal tissue, by<br/>9<br/>employing Roche 454 pyrosequencing producing one of the largest cDNA libraries for a crustacean species. A total of 12,645 gonadal contigs were assembled from 213,212 sequencing reads, and 1206, 1745 and 782 contigs were found to be male-, female- and …
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Bacterial Diversity of the Atacama Desert, Chile: The Challenges of Characterizing the Community Dynamics of Extreme Oligotrophic Ecosystems
… analysis included PCR-DGGE, Sanger-clone and 454-pyrosequencing analysis of 16S rRNA gene libraries, and the use of reverse transcriptase quantitative PCR (RT-qPCR) for quantifying the impact of environmental variables on the metabolic activity of a specific organism. These techniques were …
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Assembly and Automated Annotation of the <i>Clostridium scatologenes</i> Genome
… Applied Biosystems Solid method and the Roche 454 pyrosequencing method. The resulting DNA sequences were combined and assembled into 8267 contigs with an average length of 1250 bp with the Newbler Assembler program. Comparision of published subunits of csd gene and assembled contigs identified …
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A metagenomic approach to understanding the relationship between the vaginal microbiome and women’s reproductive health
… test to measure the sialidase activity, 454-pyrosequencing of amplicons from the V1-V3 region of the 16S rRNA gene to classify sequence to bacteria, and Illumina high-throughput sequencing to classify sequence to bacteria and functional genes in microbial samples from 38 Chinese women …
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From single genomes to natural microbial communities : novel methods for the high-throughput analysis of genomic sequences
… particular, the recently developed, ultra-fast 454 pyrosequencing has dramatically reduced the cost and time requirements per sequenced base pair. Furthermore, novel methods have been developed that enable sequencing of the 99 percent of microbes that are difficult to access with conventional, …
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