Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"4-thiouridine"”.
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Developing a Novel Cell Surface RNA Detecting and Profiling Method via RNA Metabolic Labeling
… that were used to label such csRNAs are 4-Thiouridine (4sU), an unnatural nucleotide analogue, and S-(2-aminoethyl)-ester-methanesulfonothioic-acid-biotin (MTSEA-biotin), a crosslinker designed specifically to label 4sU. By deploying these tools to cell lines such as HEK293T and HeLa, csRNA …
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Biochemical and genetic characterization of mercaptopyruvate sulfurtransferase and paralogous putative sulfurtransferases of Escherichia coli
… is necessary for biosynthesis of thiamin and 4-thiouridine in transfer ribonucleic acid. These observations led to the hypothesis that sulfurtransferases might be involved in sulfur transfer for biosynthesis of some sulfur-containing cofactors (e.g., biotin, lipoic acid, thiamin and …
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Biochemical analysis of the interaction between transfer ribonucleic acid and exportin-t
… the contact sites between tRNA and exportin-t. 4-Thiouridine (s4U) was introduced into tRNAPheT.th by in vitro transcription with T7 RNA polymerase and crosslinked to exportin-t by irradiation at 312 nm. The crosslinking was Ran•GTP dependent and could be competitively inhibited by other tRNA …
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Mechanisms of CD8+ T Cell Mediated Virus Inhibition in HIV-1 Virus Controllers
… T-cells with Gag p24 and Nef peptides adding 4-thiouridine (4SU) during the final hour of stimulation, allowing for separation of RNA made during the final hour of stimulation. Subsequent PCR of RNA isolated from these cells, allowed us to determine how much mRNA was made for our genes of …
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Versuche zur Strukturaufklärung bakterieller Thiouridin Synthetasen
Von nahezu allen rezenten Organismen ist bekannt, dass ihre tRNA posttrans-kriptional modifiziert wird. Ein Beispiel für diese Modifikationen ist die Insertion von Schwefel, wie sie durch die beiden Sulfurtransferasen ThiI und MnmA katalysiert wird. ThiI katalysiert die Insertion von Schwefel an …