Global ETD Search
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Showing 1 to 20 of 65 for “"3T3-L1"”.
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MicroRNAs: Tissue Expression and Role in 3T3-L1 Pre-Adipocyte Differentiation
… We investigated whether microRNAs regulate 3T3-L1 pre-adipocyte differentiation. The expression of microRNAs during 3T3-L1 pre-adipocyte differentiation was detected by microarrays and confirmed by northern blot and quantitative real time PCR. Several microRNAs, including let-7, were …
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Characterisation of the role of AMP-activated protein kinase in 3T3-L1 adipocytes
… inhibits insulin-stimulated glucose transport in 3T3-L1 adipocytes, which is in contrast to the effect of AICAR in skeletal muscle. Currently the role of AMPK in adipocytes remains poorly characterised despite the importance of fat tissue in energy homeostasis. To address this, the molecular …
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An Investigation into the Role of Arf6 in MCH-mediated Rearrangements in 3T3-L1 Preadipocytes
… may result in destabilized actin in murine 3T3-L1 pre-adipocytes. A pharmaceutical inhibitor of Arf6, NAV-2729, as well as the overexpression of dominant negative ARNO/Arf6 plasmids were used to determine if Arf6 was indeed a downstream signaling component in this pathway. Fluorescence …
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Proteomics of Oxidative Stress Using Inducible CYP2E1 Expressing HepG2 Cells and 3T3-L1 Adipocytes as Model Systems
… stress related changes to the proteomes of 3T3-L1 adipocytes and an inducible CYP2E1 expressing HepG2 cells. Enhanced oxidative stress in hypertrophic adipocytes is associated with metabolic dysregulation and insulin resistance. Because mitochondria generate reactive oxygen species (ROS), we …
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Regulação da adipogênese e da secreção de quemerina por ácidos graxos de cadeia média e pelo ácido graxo eicosapentaenóico em células 3T3-L1
… (C8:0 e C12:0) sobre a adipogênese em células 3T3-L1. Além disso, avaliou-se o efeito desses ácidos graxos sobre a expressão de mRNA e secreção de quemerina, uma nova adipocina envolvida na adipogênese. Para isso, preadipócitos foram cultivados em presença (adipogênese induzida) ou ausência …
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Altered regulation of adipogenesis with respect to disease processes
… on the expression profile of differentiating 3T3-L1 mouse preadipocytes and identified over 200 transcripts whose expression was altered. Four of these genes namely ITM2A, IGFBP5, PTPRQ and WNT6 were selected for detailed investigation using the 3T3-L1 in-vitro adipogenesis model. Preliminary …
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The role of sex steroids in the regulation of the Obese gene and leptin secretion
… in cultured cells. A mouse adipocyte cell line (3T3-L1) was treated with 17β-estradiol and dihydrotestosterone and RNA was harvested for analysis by qRT-PCR. Media extracts were also collected for leptin ELISA. The present study suggests a weak stimulation of Obese mRNA accumulation by estrogen …
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Some bioactivity of triterpenes from stem bark of protorhus longifolia and their derivatives
… on glucose uptake in C2C12 muscle cells and 3T3-L1 adipocytes, and triglyceride accumulation in 3T3-L1 adipocytes was also investigated. The anticoagulant and anti-inflammatory activity was investigated using tail bleeding time assay and cotton pellet-induced granuloma model in Sprague-Dawley …
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Quantitation, Distribution and Secretion of Plasma Phospholipid Transfer Protein
Secretion of PLTP by mouse 3T3-L1 preadipocytes was also investigated. Characteristics of mouse adipocyte-secreted PLTP were similar to those of human plasma PLTP. Secretion of mouse PC transfer activity increased after differentiation.
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1,25-Dihydroxyvitamin D Regulation of Triacylglycerol Accumulation in Differentiated Adipocytes
… in differentiated adipocytes. To study this, 3T3-L1 adipocytes were differentiated for 9 days, followed by stimulation with 1,25(OH)2D (10 nM) or vehicle for 1-7 days. Results indicate that 1,25(OH)2D stimulates a 21% reduction in TAG accumulation in differentiated 3T3-L1 adipocytes after 4 …
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An in vitro study of the effects of free fatty acids on insulin mediated glucose uptake and metabolism by myocytes and fibroblast derived adipocytes
… and myotubules by serum deprivation, while mouse 3T3-L1 fibroblasts were differentiated into adipocytes by culturing in 3-isobutyl-1-methylxanthine (IBMX), dexamenthasone and insulin. Myocytes and adpipocytes were cultured in DMEM containing 5.5 mM or 20 mM glucose supplemented with 0.75 mM of …
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Assessment of chemical markers as surrogates for efficacy and safety of Rooibos extracts
… uptake and lipid accumulation was also tested in 3T3-L1 adipocytes. GRE’s were also tested at concentrations ranging from 1 and 100 μg/mL for inhibitory activity against the protein tyrosine phosphatase 1B (PTP1B) enzyme. To investigate the possible mechanism of action of glucose uptake in C2C12 …
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Study of the antioxidant, anti-inflammatory and anti-diabetic effects of extracts from Cotinus coggygria in models with experimental animals and cell cultures // Проучване на антиоксидантното,противовъзпалително и противодиабетно действие на екстракти от Cotinus coggygria в експериментални модели с опитни животни и клетъчни култури
… of extracts of C. coggygria on cell cultures: 3T3-L1 (murine preadipocytes) and J774A.1 (murine macrophages);5)treatment of male Wistar rats with three aqueous extract from the leaves and aqueous-ethanolic extract of wood in a model of subchronic toxicity (30 days); (6) Study of in vivo …
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The impact of whey protein isolate on energy balance regulation
… lysophosphatidic acid receptor 5 (LPA5) in 3T3-L1 pre-adipocytes was assessed. Over-expression of the receptor in 3T3-L1 pre-adipocytes provided a growth advantage to the cells and suppressed cellular differentiation into mature fat cells. In conclusion, the data demonstrates WPI impacts on …
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Stanovení biologické aktivity rekombinantního proteinu adiponektinu pomocí buněčné kultury
… práci s buněčnými kulturami konkrétně s linií 3T3-L1. Na základě proliferačních testů byla vybrána koncentrace inaktivovaného séra, která byla používána pro všechny další experimenty. V proliferačním testu byla ověřena stimulace růstu buněk pomocí růstových faktorů HB-EGF, PDGF-BB a bFGF v …
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Mechanistic Investigation of Genotype-Phenotype Correlations in PIK3R1-Related Diseases
… mutations in insulin-responsive cell types, 3T3-L1 preadipocyte models with conditional overexpression of p85$\alpha$ Y657X or p85$\alpha$ R649W were generated. Doxycycline-induced overexpression of mutant p85$\alpha$ attenuated insulin-stimulated Akt phosphorylation due to reduced …
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Defining the metabolic effect of peroxisome proliferator-activated receptor δ activation
… To achieve this, the metabolic phenotype of the 3T3-L1 adipocyte cell line was defined to generate a metabolically phenotyped in vitro model of adipose tissue. The importance of fatty acid alpha-oxidation in the differentiation of adipocytes was emphasised The effects of PPAR delta and PPAR gamma …
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The role of adiponectin and the adipocyte in energy metabolism and inflammation
… on insulin sensitivity in pig adipocytes, and 3T3-L1 adipocytes were examined. In pig adipocytes, concentrations of 25 and 40 mM glucose inhibited insulin stimulated glucose uptake (P < 0.05), that could not be reversed by addition of adiponectin to the culture media. To examine if …
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