Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 172 for “"3T3"”.
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MicroRNAs: Tissue Expression and Role in 3T3-L1 Pre-Adipocyte Differentiation
… We investigated whether microRNAs regulate 3T3-L1 pre-adipocyte differentiation. The expression of microRNAs during 3T3-L1 pre-adipocyte differentiation was detected by microarrays and confirmed by northern blot and quantitative real time PCR. Several microRNAs, including let-7, were …
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Characterisation of the role of AMP-activated protein kinase in 3T3-L1 adipocytes
… inhibits insulin-stimulated glucose transport in 3T3-L1 adipocytes, which is in contrast to the effect of AICAR in skeletal muscle. Currently the role of AMPK in adipocytes remains poorly characterised despite the importance of fat tissue in energy homeostasis. To address this, the molecular …
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An Investigation into the Role of Arf6 in MCH-mediated Rearrangements in 3T3-L1 Preadipocytes
… may result in destabilized actin in murine 3T3-L1 pre-adipocytes. A pharmaceutical inhibitor of Arf6, NAV-2729, as well as the overexpression of dominant negative ARNO/Arf6 plasmids were used to determine if Arf6 was indeed a downstream signaling component in this pathway. Fluorescence …
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Ultrasonic backscatter coefficient quantitative estimates from Chinese hamster ovary and BALB/3T3 cell pellet biophantoms
… Chinese hamster ovary (CHO), and new and old 3T3 cells. Also introduced, for its geometrical similarities to eukaryotic cells, is a concentric sphere scattering model. BSC estimates from cell pellet biophantoms of known number density were performed with 20, 40 and 80 MHz focused transducers …
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Proteomics of Oxidative Stress Using Inducible CYP2E1 Expressing HepG2 Cells and 3T3-L1 Adipocytes as Model Systems
… stress related changes to the proteomes of 3T3-L1 adipocytes and an inducible CYP2E1 expressing HepG2 cells. Enhanced oxidative stress in hypertrophic adipocytes is associated with metabolic dysregulation and insulin resistance. Because mitochondria generate reactive oxygen species (ROS), we …
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Die Rolle einer konstitutiv-aktiven MAP-Kinase SAPK-Beta bei der zellulären Transformation, Proliferation und Apoptose von NIH-3T3-Fibroblasten
… zur morphologischen Transformation von NIH 3T3 Fibroblasten. Darüber hinaus bildeten diese Zellen kleine Foci aus transformierten Zellen, wuchsen in Soft-Agar und vergleichbar mit onkogenem Ras oder Raf, resultierte auch die Expression von aktiviertem SAPKb in der Zerstörung des F-Aktins. …
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Regulação da adipogênese e da secreção de quemerina por ácidos graxos de cadeia média e pelo ácido graxo eicosapentaenóico em células 3T3-L1
… (C8:0 e C12:0) sobre a adipogênese em células 3T3-L1. Além disso, avaliou-se o efeito desses ácidos graxos sobre a expressão de mRNA e secreção de quemerina, uma nova adipocina envolvida na adipogênese. Para isso, preadipócitos foram cultivados em presença (adipogênese induzida) ou ausência …
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Altered regulation of adipogenesis with respect to disease processes
… on the expression profile of differentiating 3T3-L1 mouse preadipocytes and identified over 200 transcripts whose expression was altered. Four of these genes namely ITM2A, IGFBP5, PTPRQ and WNT6 were selected for detailed investigation using the 3T3-L1 in-vitro adipogenesis model. Preliminary …
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Novel Metabolic Regulation of Invadopodia and Invasion by Acetyl-CoA Carboxylase and Lipogenesis
… reduced invadopodia incidence in Src-transformed 3T3 (3T3-Src) cells. Blocking ACC or fatty acid synthase (FASN) activity also altered pathway activity and cellular phospholipid profiles, including that of phosphatidylcholine (PC). Exogenous supplementation with fatty acid or PC following ACC …
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Coating of Polyvinylchloride for Reduced Cell / Bacterial Adhesion and Antibacterial Properties
… for polymers with antibacterial properties. 3T3 mouse fibroblast cells were used for cell adhesion, Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus were used for bacterial adhesion in the first study, Pseudomonas aeruginosa and Staphylococcus aureus were used for bacterial …
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Studies of SV40-transformation and the Loss of Growth Factor Requirements
… requirements. In particular, SV40 transformed 3T3 fibroblasts had a significantly reduced growth requirement for insulin. A reduced insulin requirement was also observed in several other transformed cell lines. Dose response studies with insulin and insulin-like growth factors indicated that …
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The role of sex steroids in the regulation of the Obese gene and leptin secretion
… in cultured cells. A mouse adipocyte cell line (3T3-L1) was treated with 17β-estradiol and dihydrotestosterone and RNA was harvested for analysis by qRT-PCR. Media extracts were also collected for leptin ELISA. The present study suggests a weak stimulation of Obese mRNA accumulation by estrogen …
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Primary culture of human limbal-corneal epithelial cells under different conditions
… stem-cell biology in vitro with and without 3T3 feeder cells. In particular, the expression of stem cell markers and colony-forming efficiency in different culture models were investigated. The research can be divided into three sections a) development of tissue culture models, b) translation …
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Some bioactivity of triterpenes from stem bark of protorhus longifolia and their derivatives
… on glucose uptake in C2C12 muscle cells and 3T3-L1 adipocytes, and triglyceride accumulation in 3T3-L1 adipocytes was also investigated. The anticoagulant and anti-inflammatory activity was investigated using tail bleeding time assay and cotton pellet-induced granuloma model in Sprague-Dawley …
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ΟΙ ΣΥΝΕΠΕΙΕΣ ΤΗΣ ΕΚΦΡΑΣΗΣ ΤΗΣ ΠΡΩΤΕΙΝΗΣ Ρ21 ΓΟΝΙΔΙΑΚΟΥ ΠΡΟΙΟΝΤΟΣ ΤΟΥ ΟΓΚΟΓΟΝΙΔΙΟΥ Η-RAS1 ΚΑΤΑ ΤΗΝ ΚΑΡΚΙΝΟΓΕΝΕΣΗ
… EARLY PASSAGE BALB/C EMBRYO CELLS AND BALB/C 3T3 CELLS TRANSFORMED BY THE HUMAN T24 H-RAS ONCOGENE. THE PRESENCE OF AN EXCESS OF NORMAL CELLS COULD SUPPRESS THE PHENOTYPE OF TRANSFORMED CELLS IN VITRO. A SIMILAR TYPE OF SUPPRESSION BY NORMAL CELLS COULDBE SHOWN IN VIVO IN TUMOURS INDUCED BY …
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Quantitation, Distribution and Secretion of Plasma Phospholipid Transfer Protein
Secretion of PLTP by mouse 3T3-L1 preadipocytes was also investigated. Characteristics of mouse adipocyte-secreted PLTP were similar to those of human plasma PLTP. Secretion of mouse PC transfer activity increased after differentiation.
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A Role for RAS in V-CRK Transformation
… by a different mechanism. v-Crk transformed NIH-3T3 cell lines were established in order to examine the effects of blocking the Ras pathway in v-Crk transformed cells. In contrast to previous studies done in mammalian cells, v-Crk caused morphological transformation and promoted …
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