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Showing 1 to 6 of 6 for “"3D8 single chain variable fragment (scFv)"”.
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Generation of chickenized 3D8 scFv antibody from mouse monoclonal anti-DNA antibody by CDRs-grafting
3D8 single chain variable fragment (scFv) derived from a MRL-lpr/lpr mouse is a catalyric anti nucleic acid antibody that has ability of cellular internalization and DNA/RNA-hydrolyzing activity. It has been reported that cells treated with 3D8 scFv protein showed the down-regulation against …
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Identification of the receptor for cellular internalization of an anti-nucleic acid antibody, 3D8 scFv
… of cells. An anti-nucleic acid antibody, murine 3D8 single chain variable fragment (scFv) Ab, has been known to be an internalizing antibody that enters the cells by caveolae/lipid-raft-mediated endocytosis. However, cell surface receptor for 3D8 scFv internalization remains unknown. Here, we …
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Functional stability of a nucleic acid-hydrolyzing single chain antibody in various biochemical and biophysical environments
3D8 single chain variable fragment (scFv) antibody has cell-penetrating activity, nucleic acid-binding activity, nucleic acid-hydrolyzing activity, and antiviral activity. As a first step toward development of antiviral reagent using 3D8 scFv, functional stability based on DNA-binding and …
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Suppressive effect of a nucleic acid-hydrolyzing recombinant antibody (3D8) on viral multiplication
3D8 single chain variable fragment (scFv) is a recombinant anti-DNA antibody from an autoimmune-prone MRL-lpr/lpr mouse with lupus diseases. 3D8 scFv can hydrolyze ss-DNA, ds-DNA, ss-RNA, and ds-RNA without sequence specificity. On the base of 3D8 scFv property, we carried out quantitative and …
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Antiviral activity of 3D8 single chain Fv against RNA virus infection
… fields to target viral antigens effectively. 3D8 is an anti-DNA catalytic antibody that binds to DNA and RNA then degrades them without sequence specificity as well known as Abzymes. Previously it was reported that 3D8 single chain variable fragment (scFv) has cell-penetrating activity by …
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Effect of disruption of 3D8 complementarity-determining regions on properties of 3D8 antibody
… CDR-disruption on the function of an antibody, 3D8 scFv (single chain variable fragment; variable region of heavy chain connected to variable region of light chain by (G4S1)3 linker) which has DNA-binding/-hydrolyzing, heparin-binding and cell-penetrating activities. We generated single …