Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"3D-cultures"”.
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Metabolic-response assessment of metastatic murine breast cancer in 2D and 3D cultures using intrinsic NADH as a natural biomarker
… are distinctly different in threedimensional (3D), tumor-like model as compared with that of the conventional 2D monolayer culture. To test this hypothesis, we employed two-photon (2P) fluorescence lifetime imaging microscopy (2P-FLIM) and time-resolved 2P-fluorescence anisotropy of the reduced …
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Use of a 3D liver microreactor as an in vitro model for the study of bile acid synthesis and hepatobiliary circulation
… etc. This thesis focuses on the use of a 3D in vitro model of liver to study the effects of compounds and culture conditions on hepatobiliary transport and bile acid synthesis. In order to achieve this goal, protocols were developed to perform hepatic transport studies of bile acids in …
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Design of three-dimensional axon stretch growth device
… are stretched grown by using three-dimensional (3D) cultures. This thesis work involves modification of the existing 2D axon stretch growth device to achieve axon growth in 3D cultures. The design includes separating two 3D hydrogel cell cultures using a porous nylon mesh to constrain each half …
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Development of collagen-based scaffolds for differentiation of induced pluripotent stem cells
… been broadly studied and applied in engineering 3D scaffold materials in tissue engineering. A collagen hydrogel can provide cells with a porous and soft environment to proliferate and differentiate. However, lacking mechanical stiffness and shrinkage resistance made it a challenge to sustain …
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Factors affecting bone cell growth and differentiation under differing culture conditions
… with bone grafts, implantation of biodegradable 3D scaffolds seeded with MSCs has been suggested. However, differences in osteoblast differentiation in 2D versus 3D cultures remain unclear. In this study, rat marrow stromal cells (MSCs) were grown both on plastic and in 3D polymer scaffolds and …
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Elucidating the Role of Cellular Architecture in the Developing Pancreas
… have improved upon transitioning from 2D to 3D cultures, indicating that the 3D microenvironment in which β cells are born is critical for the acquisition of their cell fate. However, little is known about the mechanisms through which the 3D architecture of the developing pancreas mediates …
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Investigation of pancreatic beta cell differentiation and function, and global profiling analysis of diabetes serum for the identification of disease biomarkers
… iPS cell line was performed in 2D and 3D culture, with improved efficiency of definitive endoderm formation in 3D cultures (a step on the differentiation routetowards beta cells). In parallel, serum from diabetes patients was examined to identify new disease iomarkers in a collaborative …
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Engineering Matrix Properties for Stem Cell Culture and Gene Delivery
… both two-dimensional (2D) and three-dimensional (3D) cultures, and the proliferation of SSCs was influenced by the density of cell adhesion ligands but not by substrate stiffness. Separately, the effects of substrate stiffness on non-viral gene delivery were evaluated for stem and precursor cells …
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Advanced Microfluidic Platforms for Studying the Stromal Compartment of Pancreatic Cancer Tumor Microenvironments
… combination of microfluidic technologies with 3D cultures have resulted in Organ-on-a-Chip (OOC) technologies that leverage the microscale manipulation of fluids to achieve precise spatiotemporal control of 3D tissues. However, many of these systems remain in the proof-of-concept stage due to …
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A 3D synthetic extracellular matrix that can be dissolved to interrogate the cellular local microenvironment on demand
In vitro multi-cellular 3D cultures can provide tremendous insight into pathologies arising from dysregulation of extracellular communication by recapitulating key features of the microenvironment, such as ECM-cytokine interactions, spatially regulated cell-cell interactions, and matrix biophysical …
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Attributes of Astrocyte Response to Mechano-Stimulation by High-Rate Overpressure
… to astrocyte response and reactivity. Astrocyte cultures were exposed to a 20 psi high-rate overpressure scheme using an underwater explosion-driven device. Astrocytes experienced dynamic energetic fluctuations in response to overpressure which were followed by the assumption of a classically …
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RESOLVING TUMOR HETEROGENEITY THROUGH HIGH RESOLUTION MULTI-MODAL TRANSCRIPTOMICS: INTEGRATIVE SINGLE-CELL ATLASES AND GENERATIVE SPATIAL MODELING
… Using single-cell RNA-seq, I profiled 2D and 3D cultures grown in the presence or absence of ascitic fluid (AF). AF supplementation enhanced proliferation, preserved minor tumor populations, and reshaped the composition of cancer-associated fibroblasts (CAFs). The transcriptomic landscape of …
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ADULT HUMAN BRAIN MENINGES HOST NEURAL STEM/PRECURSOR CELLS THAT CAN BE IN VITRO EXPANDED AND SHOW MULTIPOTENT NEURAL DIFFERENTIATION POTENTIAL IN 2D AND 3D CULTURE
… and assessed NSC properties in vitro in 2D and 3D culture. To perform this, we first set up the methodologies for brain meninges cell isolation, and characterization of NSC in vitro properties, differentiation in 2D and 3D culture and transplantation in vivo using the murine models. We observed …
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A noninvasive screening tool for cell fate determination using raman spectroscopy and machine learning in tissue engineering and microbiology applications
… these stem cells to self-renew in artificial cultures. Though various cellular, biochemical, and mechanical cues regulate the fates of HSCs in their native microenvironment, attempts to use niche-inspired cues to expand and maintain HSCs in vitro for extended times have had limited success. …
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Hypoxia and Hypoxia-Inducible Factors in Normal and Malignant Breast Epithelium
… mammary cells grown in three-dimensional cultures. Heterogeneous ER expression in breast cancer was additionally found to be related to cyclin D1, a cell cycle regulator frequently overexpressed in breast cancer. As cyclin D1 expression was not affected by hypoxia, our findings suggest two …
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The role of mechanical loading in chondrocyte signaling pathways
… We also developed a three-dimensional (3D) cell culture system using collagen-coupled agarose gels to mimic the physiologically relevant cell microenvironment. The activities of Lyn-Src, KRas-Src and Lyn-FAK were up and down regulated by high (>10 μl/min) and moderate (5 μl/min) …
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Surface functionalized iron oxide nanoparticles for applications in biomedical sciences
… nanoparticles is examined in two methods, 2D and 3D cell culturing. By analyzing the cell response in 2D and 3D cultures an accurate estimation of the toxicity of nanoparticles can be made. The toxicity of iron oxide was assessed using commercially available cell assays (CellTiter-Glo and …
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The Use of 2D and 3D Models to Study Competition in the Context of Barrett’s Oesophagus and Oesophageal Adenocarcinoma
… organoids were utilised to study competition in 3D. Co-cultures were generated from fluorescently tagged BO and OAC organoids and imaged for analysis. Fusion events between BO and OAC organoids were captured by wide-field microscopy and quantified, providing an additional method to study …