Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"3D spheroids"”.
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Identifcation and stablization of a novel 3D hepatocyte monolayer for hepatocyte-based applications
… morphological stage before the formation of 3D spheroids was discovered when the hepatocytes attach to the substrata as monolayer but exhibiting many characteristics of the 3D spheroids, but without the limitations of the 3D spheroids. We coined the name of b 3D hepatocyte monolayerb to …
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Effect of Mesoporous Silica-Loaded Anticancer Drugs on 3D vs 2D Models of Colon Cancer Cells
… drugs warrants evaluation in a suitable 3D model mimicking in vivo tumours. This study investigated the impact of a potential chalcone-based anticancer drug, DMU121, and oxaliplatin (OXA) used in treating CRC. Both anticancer drugs were loaded onto mesoporous silica nanoparticles (MSNs) …
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The Influence of Human Umbilical Vein Endothelial Cells in the Formation of Glioblastoma Spheroids in Three-Dimensional Microwells
… However they may not provide threedimensional (3D) characteristics of the tumors. Therefore, there is an increasing need in 3D cell culture models to investigate cell growth and cell-cell interactions. In this thesis, we hypothesized that culturing GBM cells with endothelial cells in the …
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AI Assisted Cellular and 3D Spheroid Analyses for Combined Therapies Against Glioblastoma
… with lenalidomide (RT+Len, immunotherapy) in 3D spheroids, and temozolomide with graphene quantum dots (TMZ+GQD). Microscopy images at 24 and 48 hours were analyzed using k-means and hierarchical clustering with no labels or predetermined features. For RT+Vor, machine learning identified …
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Development of advanced 3D tissue models and O2 imaging methodologies
… they showed minimal toxicity and could penetrate 3D tumour spheroids in depth, showing efficient staining and even distribution. We applied the imaging methodology to the 3D spheroid model, investigating which method of formation from "free floating", "hanging drop" and LipidureTM, produced the …
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Modeling mammary epithelial cell polarization and the role of podocalyxin in breast tumor progression
… vitro by culturing mammary epithelial cells as 3D spheroids embedded in a reconstituted basement membrane. I first used this assay to characterize the role of cell-cell and cell-ECM adhesion in the formation and polarization of the apical junction complexes in normal mammary epithelial cells. …
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ELUCIDATING THE IMPACT OF ASCITES ON HIGH GRADE SEROUS OVARIAN CANCER THROUGH MULTIPLE IN VITRO PATIENT-SPECIFIC MODELS
… in vitro models, specifically 2D cells, 3D stem-enriched spheroids and polyclonal organoids, to develop preclinical models able to capture and faithfully reproduce the heterogeneity and complexity of this tumor and its TME. We could show that AF supplementation improves culturing …
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Metabolic Adaptations of Ovarian Cancer Metastases to Physiological Conditions and Disease Progression
… from the primary tumor and aggregate to form spheroids, enhancing their survival within the peritoneal cavity during dissemination to a secondary outgrowth site. The inability of removal of these aggregates by traditional surgical interventions may contribute to the high recurrence and …
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Targeting LGALS3 to treat steatotic liver disease and hepatocarcinogenesis
… by the employment of the gene silencing in a 3D in vitro model of steatosis. The same model was also used to verify the in vitro effect of T3 and MGL-3196 (a selective agonist of the thyroid hormone receptor β-isoform) on neutral lipid content and collagen accumulation. The gene silencing was …
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Development of new photoluminescent sensing materials, systems and applications for cell analysis
… and demonstrated with cultured cells and 3D spheroids made of HCT 116 cells, measuring their ECAR/OCR and responses to stimulation. Finally, the planar solid-state pH sensors on polyester films (Mylar) were used to measure extracellular pH in grafted tumours produced by CT26 cells and …
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Rational identification of a dual inhibitor of PARP-1 and ATM kinase
… in patient derived Glioblastoma stem cells and 3D spheroids. I also performed target validation experiments looking at the interplay between both targets, the role they play in the DDR and the possible mechanism(s) behind their synthetic lethality. An effort was undertaken to identify a PARP-ATM …