Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"3D genome"”.
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How Chromosome-Nuclear Envelope Attachments Affect 3D Genome Organization
… for gene expression and regulation. Thus, genome sequences must be understood in the context of their 3D organization which critically influences the flow of information. The effort to understand this added complexity now encompasses an entire field of chromosome biology and is reshaping …
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New insights into 3D genome architecture through improved genomic methods
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2027-05-01
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Analysis of 3D Genome Organization in 4-Cell Mouse Embryos
… coupling between the spatial organization of the genome inside the cell nucleus and a cell’s gene expression profile, in order to gain insights into cell fate determination, we studied the 3D genome organization; in particular how chromosomes are arranged in the 3D space of the cell nucleus. More …
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Study of 3D Genome Organisation in Budding Yeast by Heterogeneous Polymer Simulations
… the nucleus has revealed the essential role of genome organisation in controlling genome function. Furthermore, genome architecture is highly dynamic and significant chromatin re-organisation occurs in response to environmental changes. However, the mechanisms that drive the 3D organisation of …
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Computational methods for analyzing and modeling gene regulation and 3D genome organization
… analyzing and linking gene expression with the 3D organization of the genome. First, I describe methodologies for elucidating gene regulatory mechanisms by considering multiple data modalities. I design a computational framework for identifying colocalized and coregulated chromosome regions by …
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The 3d Genome As A New Dimension In Understanding Pathologic Short Tandem Repeat Instability
… of repetitive sequences as well as other genome dysfunction such as disrupted expression of the gene containing the repeat. Recently, the spatial positioning of chromatin in 3D space has emerged as a precisely regulated process that has functional consequences for development and disease. …
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Investigating 3D genome organisation in Caenorhabditis elegans with Accessible Region Conformation Capture (ARC-C)
… of Hi-C, only survey a pre-defined subset of the genome. I developed a variant of Hi-C, named Accessible Region Conformation Capture (ARC-C), in C. elegans, which interrogates genome organisation at multiple scales genome-wide - from domains and compartments to high resolution interactions between …
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The role of MLL4 in the chromatin framework: from the epigenome to 3D genome organization
… haploinsufficiency, TrxG members show reduced genome binding, while PcG strengthens its enrichment and genome occupancy. This chromatin state rearrangement is also reflected on a three-dimensional scale. Genome Architecture Mapping (GAM) experiments reveal distinct changes in 3D chromatin …
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Building the 3D Genome from the Ground Up: Local Interactions Give Rise to Global Order
The three-dimensional organization of the genome within the nucleus plays a central role in determining gene regulation and establishing cellular identity, but the mechanisms by which local molecular interactions give rise to global chromatin architecture remain an active area of study. …
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Dissecting The Roles of Human Smc Complexes In Transcription Regulation and Chromatin Organization
… elements and genes in the three-dimensional (3D) genome. However, the hierarchy of chromatin contacts and their underlying mechanisms are not well-understood. SMC complexes including Cohesin complex and Condensin complex has been widely proposed to organize 3D genome structure, and further …
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"""TSA-Seq"": a novel proximity mapping approach for studying three dimensional genome organization and function"
… 2010) and is important for maintaining normal genome function. However, although it is known that the distribution of chromatin and genes in the eukaryotic cell nucleus is non-random and dynamic, the actual domain organization of chromatin, the mechanisms and dynamics which give rise to this …
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Sculpting 3d Chromatin Folding Via Genome Editing
… inserts revealed their distinct contributions to genome folding. Altogether, short DNA insertions can suffice to shape the spatial genome in a manner influenced by chromatin context. Additionally, we frame our findings in the context of recent advances in experimentally creating chromatin loops, …
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The role of the SWI/SNF ATP dependent chromatin remodelling complex in the regulation of the human hair follicle cell proliferation and control of the human cutaneous wound healing
… as well as higher order chromatin folding and 3D genome organisation. ATP-dependent chromatin remodelling complexes modulate nucleosome structure, positioning and chromatin de-compaction and are involved in gene activation and repression. SWI/SNF ATP-dependent chromatin remodelling complexes …
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Characterizing 3D epigenomes in pathological conditions
<p>The human genome is intricately folded within the confines of a minuscule nucleus, maintaining critical activities, including transcription, replication, and DNA repair. These processes are orchestrated by the epigenome. Prior research has established that both the epigenome and its …
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Mechanisms of genomic stability maintenance in fission yeast
… in order to achieve timely duplication of the genome in the timeframe of S. The subset of origins activated in each cell cycle constitute the replication program of the cell. Despite the fact that origin selection is characterized by stochasticity, there are several parameters affecting the …
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Molecular Mechanisms Behind Sars-Cov-2 Induced Host Genome Restructuring
… at least in part, to significant alterations in 3D genome organization and epigenetic landscape following SARS-CoV-2 infection that are distinct from and more severe than changes caused by other viral infections or immune stimulants. While these changes at the chromatin level are important for …
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Data-driven Mechanistic Modeling of 3D Human Genome
Three-dimensional (3D) organization of the human genome regulates DNA-templated processes, including gene transcription, gene regulation, and DNA replication, which are crucial for cell differentiation and cell functionality. Computational modeling serves as an efficient and effective way of …
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The impact of chronic loss of STAG2 on DNA damage repair and the epigenome in acute myeloid leukaemia
… chromatid cohesion as well as organizing the 3D genome, controlling gene expression and DNA repair. The consequences of a STAG2 mutation on Cohesin function is not well understood and in particular, the effects on DNA damage repair remain to be fully elucidated. In addition, it is likely that …
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Insights into transcriptional regulation from natural and induced variation in closely related species
… functional contexts, such as higher-order genome organisation or the neoplastic transformation of a tumour cell in cancer development. In the first part of the thesis, I explore the evolutionary dynamics of the CTCF (CCCTC-binding factor) binding among mouse species and its interplay with …
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The evolution of gene regulatory landscapes in mammalian tissues
… studied. To fill this gap, I analyzed whole-genome bisulfite sequencing (WGBS) and matched chromatin immunoprecipitation assays followed by sequencing (ChIP-seq) of five TFs from the livers of five mammals. I first characterized DNA methylation patterns at transcription factor binding regions …