Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 73 for “"3D culture"”.
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The effect of hypoxia and 3D culture conditions on heterogeneous ovarian cancer spheroids
… metastasis potential of ovarian cancer. SVF co-cultured spheroids further increased the glycolytic shift of the heterogeneous of ovarian cancer spheroids, induced the aggressive phenotype by elevating the corresponding protein markers. Decreasing the glycolytic shift and suppression of the …
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DIFFERENTIATION OF NEURAL STEM CELL USING SMALL MOLECULES IN 2D AND 3D CULTURE SYSTEM
… on the cells grown in three dimensional (3D) hydrogel or two dimensional (2D) environment since 3D hydrogel mimics the extracellular matrix and provides physiologically more relevant environment than 2D cell culture system. Three-dimensional (3D) hydrogel systems in this study involve …
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Investigating the impact of gold nanoparticles on pulmonary cells: From 2D to 3D culture systems
… how to build a 3-dimentional human lung model cultured at an air-liquid interface. Gold nanosphere aerosols were delivered to the cocultures of primary human pulmonary cells through nebulization to mimic human inhalation. Our lung model shows successful epithelium differentiation regardless of …
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The Impact of Stromal Cells on the Metabolism of Ovarian Cancer Cells in 3D Culture
… have displayed the ability to aggregate and form 3D homogeneous and heterogeneous spheroids, which can function as micrometastases. Ovarian cancer spheroids survive independently prior to adhering to an endothelial tissue. Since aggregation has been shown to provide a survival advantage to the …
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Osteogenic and chondrogenic differentiation of rat bone marrow stromal cells in 3D culture with novel PCL-TCP scaffolds
… of rat bone marrow stromal cells (BMSC) in 3D PCL-TCP scaffolds. PCL-TCP scaffolds fabricated by fused deposition modeling (FDM), are honeycombed, highly reproducible bioresorbable 3D structures with a completely interconnected pore network and custom-tailored porosity and pore size. After …
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ADULT HUMAN BRAIN MENINGES HOST NEURAL STEM/PRECURSOR CELLS THAT CAN BE IN VITRO EXPANDED AND SHOW MULTIPOTENT NEURAL DIFFERENTIATION POTENTIAL IN 2D AND 3D CULTURE
… and assessed NSC properties in vitro in 2D and 3D culture. To perform this, we first set up the methodologies for brain meninges cell isolation, and characterization of NSC in vitro properties, differentiation in 2D and 3D culture and transplantation in vivo using the murine models. We observed …
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Gain-of-Function Effects of Mutant p53 Explored Using a Three-Dimensional Culture Model of Breast Cancer
… carcinogenesis, we employed a three-dimensional (3D) culture model of breast cancer. When placed in a laminin-rich extracellular matrix, non-malignant mammary epithelial cells form structures highly reminiscent for many aspects of acinar structures found in vivo. On the other hand, breast cancer …
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The effects of BMP4 on different in vitro models for mouse gastrulation and neural induction
… induction to take place. Gastruloids are a 3D culture model that does not require BMP4 to undergo gastrulation-like events. This thesis describes the effects of BMP4 on gastruloids and on a 2D adherent cell culture model for gastrulation, demonstrating the interactions between BMP4, Nodal, …
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A Human Pluripotent Stem Cell-Derived Model for the Investigation of Thermogenic Adipocyte Development and Function
… investigates how integration of alternative cell culture systems can help optimise this in vitro BA model by potentially enabling the culture of mature adipocytes in a physiological microenvironment. Finally, this thesis examines whether the recapitulation of a physiological or pathophysiological …
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Fasting Reduces Intestinal Radiotoxicity Enabling Dose-Escalated Radiotherapy For Pancreatic Cancer
… Given this robust phenotype, we developed a 3D culture ex vivo assay using intestinal stem cell-enriched epithelial spheroid cultures. We modified these intestinal spheroids with a bioluminescent reporter and used these cells to develop a modified clonogenic assay for 3D culture that can be …
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Metabolic-response assessment of metastatic murine breast cancer in 2D and 3D cultures using intrinsic NADH as a natural biomarker
… conducted in a two-dimensional (2D) monolayer culture towards pathophysiological investigation, drug screenings, and cancer diagnostics. There is strong evidence, however, that suggests cellular behavior and metabolism in 2D cell culture is too simplistic of a model as compared with those in …
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Enhancement of death receptor-mediated apoptosis in multiple myeloma cells
… and overcome TRAIL resistance in both suspension culture, and 3D cell culture as a model of solid disseminated MM lesions that form in bone. Methods: The effects of TRAIL sensitizers and/or TRAIL treatment were investigated in both suspension cultures and in an alginate-based 3D culture model. …
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Protecting Healthy Cells Against the Negative Effects of Radiation Therapy for Lung Cancer
… damage in traditional two-dimensional (2D) cell culture conditions in vitro may not recapitulate tissue responses as modeled in three-dimensional (3D) organotypic culture systems since important signals, provided by the extracellular matrix and microenvironment, are lost in 2D monolayer cultures. …
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Peptide hydrogels as novel scaffolds for dental pulp cell culture
… 10% RGD Bioglex hydrogel were most promising for 3D culture of DPCs. <br/><br/>The DPC secretome in 3D hydrogel culture was investigated to assess the suitability of the hydrogels for engineering the dentine-pulp complex. Results showed that 0.5% w/v NapFFK’K’-OH hydrogel favoured the production …
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LINEAR AND NONLINEAR FLUORESCENCE LIFETIME IMAGING FOR BIOSENSING APPLICATIONS
… biosensing in live intact single cell within 2 D culture, 3D culture and in-vivo animal model, zebrafish.
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The Cytoplasmic Domain of Membrane-type 1 Matrix Metalloproteinase is Required for its Survival-Promoting, but not its Migration-Promoting Function in MCF-7 Breast Cancer Cells
… resulted in a protrusive cell morphology in 3D culture that was lost upon serum starvation. MT1-MMP expression in a chicken embryo tumour model resulted in vascularization of MCF-7 tumours; a phenotype that was partially maintained following expression of MT1-MMP CD variants. These results …
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Generation of human inner ear organoids from pluripotent stem cells and an organ-on-chip platform
… project builds on our previous discovery of a 3D culture system that uses hiPSCs to generate inner ear (otic) organoid containing sensory epithelium, neurons, and mesenchymal cells. We also combine novel platforms such as organoid-on-a-chip (triple-decker inserts) to enable high-resolution …
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Expression of Aldehyde dehydrogenase (ALDH) in the breast stem cell line D492 and relations to stem cell properties
… cells and forms branching colonies in 3D culture similar to structures seen in vivo. D492M is a subline of D492 that has adapted mesenchymal character through epithelial-mesenchymal transition (EMT). EMT is a process where epithelial cells gain a mesenchymal phenotype with increased …
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Influence of rearrangement of actin cytoskeleton on the overall material properties of ATDC5 cells during chondrogenesis
Studies show that when chondrocytes are cultured in 2D, they lose their phenotype. This change is reversed when the cells are embedded in a 3D culture system such as in agarose gel. Previous experiments in our lab showed that when prechodrocytic cells were embedded in agarose, chondrogenesis was …
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Microtubule Organisation in Normal and Colon Cancer Cells
… assays."" Overexpressing"cells"were"grown"in"3D"culture"to"study"any"effects"on"polarisation." The" TC7" cell" line" containing" truncated" APC" possessed" fewer" microtubules" orientated"perpendicular"to"the"cortex"than"wildNtype"expressing"cells.""This"could"be" …
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