Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 40 for “"3D cell culture"”.
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Microenvironmental modification and analysis of 3D cell culture using microgels
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01
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3D Cell Culture in Microgels: A Novel Screening Platform for Disease Modelling and Drug Testing
… drug candidates are usually tested on 2D cancer cell lines adhered to a plastic surface, which neglect many aspects of a tumour in vivo, such as cell-cell and cellmatrix interactions. In 2011, the first successful long-term culture of epithelial cells from primary tissue was developed by the …
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Microfluidic-based 3D cell culture for studies of biophysical and biochemical regulation of endothelial function
… challenge lies in replicating the native cell/tissue environment ex vivo. Certain key features of living tissues such as the three dimensionality, biophysical and biochemical microenvironment cannot be readily replicated in traditional culture platforms. Moreover, the capability for …
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Pro-oxidative and Pro-inflammatory Mechanisms of Brain Injury in Experimental Animal and 3D Cell Culture Model Systems
… include: (1) Determination of the molecular and cellular mechanisms responsible for blast- and radiation-induced brain injuries and (2) Development of a three-dimensional (3D) model system in order to mimic in vivo-like microenvironments to further broaden our knowledge in pro-oxidative and …
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Building an In-vitro Cell Model to Study Cellular Basis of Maturation Timing: A Proof of 3D Cell Culture Methodology in Atlantic Salmon Adipose Tissue
Three-dimensional (3D) cellular cultures have been shown to represent tissue formations and functions more accurately than two-dimensional (2D) cultures and have been successfully utilized more accurately in model organisms, e.g., to understand cellular modular functions. However, the applications …
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Development of single-cell Raman spectroscopy for noninvasively screening the differentiation of living stem and progenitor cells in biomaterial platforms for 2D and 3D cell culture
Stem and progenitor cells' capabilities to self-renew, differentiate, and proliferate within their native tissue made them the subject of ongoing studies to understand the mechanisms of such processes and their potential use as therapeutics. Regenerative medicine encompasses the idea of treating …
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Bioinspired soft matter self-assembly and its use in biomedical applications
… matter, and many biomedical products, such as 3D cell culture platforms or nanocarriers for drug and imaging agent delivery, often consist mostly of soft matter. Despite advances in these fields, concerns still exist regarding the function, reproducibility, and cost of soft matter systems for …
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Role of cancer stem cells in breast and prostate cancer
Cancer stem cells (CSC) are thought to be responsible for the initiation, propagation and tumour re-occurrence. CSC have been identified in most solid and haematological cancers and account for ~1% of the total cell population. Culturing cancer cell lines in monolayers enriches for the most …
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Enhancement of death receptor-mediated apoptosis in multiple myeloma cells
… and overcome TRAIL resistance in both suspension culture, and 3D cell culture as a model of solid disseminated MM lesions that form in bone. Methods: The effects of TRAIL sensitizers and/or TRAIL treatment were investigated in both suspension cultures and in an alginate-based 3D culture model. …
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The interplay of matrix rigidity and mechanical stretching for the development of artificial skin
… employed to improve tissue morphogenesis, and cellular spatial organization but it has not been previously implemented in a synergistic manner with matrix rigidity due to lack of tools in 3D cell culture systems. The goal of this study is to investigate the structural changes, the morphological …
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A Cell-Based Model to Study Factors that Drive Diffuse Astrocytoma Development
… a hypermethylator phenotype that prevents the cells from differentiating. Acquisition of additional mutations in tumor suppressors such as p53 and/or ATRX likely leads to tumor initiation. Work in the Wells Laboratory has found that loss of the HIPPO adaptor protein AmotL1 is also associated …
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Analysis of DNA damage and repair responses in cerium exposed cells and hepatocyte spheroids
… are generally performed in vitro using cultured cells to predict responses in vivo. Due to limitations of cell culture systems, there is a growing interest in utilizing 3D cell culture models to better mimic conditions found in tissues. This thesis investigates the DNA damaging potential …
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An In Vitro 3D Model to Evaluate Behaviour of Breast Cancer Cells and Response to Treatment
The field of 3D culture models of disease has started to move towards systems that aim to recapitulate the complexity of human tissues. However, despite recent improvements, current 3D systems remain overly simplistic, lacking the biophysical characteristics and diverse structures found in most …
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The Influence of Human Umbilical Vein Endothelial Cells in the Formation of Glioblastoma Spheroids in Three-Dimensional Microwells
… (OVs). The studies with two-dimensional (2D) cell cultures have been used as physiological models for understanding the cellular and molecular behavior of the GBM tumors. They are also highly desirable for virus studies. However they may not provide threedimensional (3D) characteristics of the …
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Mechanotransduction of subcellular AMPK and its role in breast cancer cell migration
… documented, yet its role in the regulation of cellular metabolism and the potential link between cellular energy and cell migration remain poorly understood. In this study, we investigated the role of mechanotransduction (via Src and FAK) in AMP-activated protein kinase (AMPK) activation in …
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Step-growth hydrogels crosslinked through grafted polypeptides enable nano- to macroscale synthetic extracellular matrix design
… secondary structure found in the native extracellular matrix. A specific grafted N-carboxyanhydride polypeptide, poly(y-propargyl-L-glutamate) (PPLG), forms the foundation of this thesis. PPLG is an especially useful polymer for exploring hydrogel crosslinking through grafted polypeptides …
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Microfluidic and optogenetic technologies to model spinal cord development and neuromuscular junction formation and function
… in the spinal cord and innervating muscle cells throughout the body are responsible for virtually all motor functions, from locomotion to respiration or speech. They arise from differentiation of progenitor cells within the neural tube under spatiotemporally well-defined morphogen …
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Protecting Healthy Cells Against the Negative Effects of Radiation Therapy for Lung Cancer
… species that can damage both cancer and healthy cells. Synthetic triterpenoids, including CDDO-Me, act as anti-inflammatory, antioxidant modulators primarily by inducing the Nrf2/ARE pathway. In the documented series of experiments, I show that CDDO-Me can be used as a radioprotector in normal …
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Global characterization of oncogenic kinase signaling
… of 235 and 261 kinases from MDA-MB-231 cells, respectively. Coupling the kinase enrichment with phosphopeptide enrichment enabled the identification of 799 kinase-derived phosphosites, with ~10% localized within the activation loop. Secondly, the global impact of constitutively active …
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