Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"3D Organoids"”.
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ADVANCING IN SITU CRYO-ET TO THE NEXT DIMENSION: TOMOGRAPHY ON 3D ORGANOIDS
… alternative is the use of three-dimensional organoids, which present a balance between physiological relevance and technical capabilities. Organoids recapitulate key aspects of tissue architecture and physiology, thus bridging the gap between cellular and tissue contexts. Additionally, they …
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IMPLEMENTATION OF 3D CELLULAR MODELS FOR DRUG AND TOXIN TESTING IN HEALTHY AND CANCEROUS TISSUE
… screening tool, we employed bioengineered 3D liv er and cardiac organoids biofabricated from multiple physiologically relevant human cell groups to represent healthy tissue , and tumor organoids derived from colorectal cancer cells from established cell lines to screen multiple materials …
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A NOVEL MAGNETIC THREE-DIMENSIONAL LEVITATED PRIMARY CELL CULTURE SYSTEM FOR THE DEVELOPMENT OF IN VITRO SALIVARY GLAND-LIKE ORGANOIDS
… cell-based approaches in three-dimensional (3D) scaffold-based systems. The majority have nonhuman-based components. Therefore, this project aims to develop SG mini-glands/organoids using a novel 3D xeno-free system (M3DL) that assembles and levitates magnetized primary SG-derived cells …
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A THREE DIMENSIONAL MODEL OF HUMAN HEPATO-BILIARY ORGANOGENESIS
… goal of this thesis project was to generate a 3D in vitro model of liver tissue using tissue engineering strategies that can recapitulate fetal liver development. There are two key components to our in vitro model: acellular liver scaffold discs that provide 3D native liver tissue …
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Integrative and Scalable Pipeline for Multi-omic Characterization of Biological Tissues
… mice, postmortem humans, and three-dimensional (3D) organoids derived from human induced pluripotent stem cells (hiPSCs), can be utilized. Regardless of the models used, a large-scale, unbiased study of the brain is yet to be achieved due to the vast complexity and high interconnectedness of the …
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Development of an in vitro human lung organoid system to study alveolar type 2 stem cell maintenance and dysfunction during disease
… developed and characterised a novel in vitro 3D organoid platform that allowed for long-term hAT2 maintenance in chemically-defined conditions. Cultured hAT2-derived organoids underwent clonal expansion, were functionally mature and exhibited maintenance of differentiation capacity. High Wnt …
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Exploring the role of BCL11A in the oesophagus and mammary gland using in vivo lineage tracing and 3D organoid models.
… in vivo lineage tracing techniques and in vitro 3D organoids to explore the expression and gene function of Bcl11a in the oesophageal epithelium and mammary gland. Gene function studies were undertaken using mouse models of BCL11A overexpression: RosaCreERT2; Rosa-CAGG-LSL-BCL11A (BCL11AOE) and …
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Developing bioelectronic sensors for 3D in vitro models at the air liquid interface: Innovative technology for biomedical and respiratory research.
… materials, microfluidics, and three dimensional (3D) organoids. However, the clinical translatability of these models is limited by the lack of sensor technologies capable of monitoring them non-invasively and in real time. Gold standard analysis techniques rely on sample labelling or destructive …
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Application and Development of Advanced Genetic Tools to Study Adult Stem Cells
… of the corpus epithelium. Gastric RZ DKO organoids show independence from the essential growth factor Rspondin-1 but require exogenous Wnt. A similar exogenous Wnt dependence was identified in a human gastric cancer cell line harbouring homozygous Rnf43 inactivating mutations. Thus, Wnt …
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OrganixInsights: High throughput imaging and high content screening of organoids
… resolution. In particular, three-dimensional (3D) organoid models have emerged as powerful experimental systems for investigating tissue development, disease progression, and therapeutic response. Organoids replicate key structural and functional characteristics of in-vivo tissues, including …
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Clonal Dynamics in Barrett's Oesophagus and Oesophageal Adenocarcinoma
… in vivo model for BO/OAC, but patient derived 3D organoids may provide an appropriate in vitro system for studying clonal dynamics, including competition between different stages of the disease. The evolution of IM through to OAC is still being explored. To date we know that progression is …