Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"35S promoter"”.
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The roles of proteinases and proteinase inhibitors in plant-nematode interactions
… suitability of cauliflower mosaic virus (CaMV) 35S promoter used for transgene constructs was evaluated in this study. It was found that the expression of proteinase inhibitors, driven by the CaMV 35S promoter, decreased in root tissues of transgenic plants during late nematode infection stage. …
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Development of genetic transformation systems in creeping bentgrass (Agrostis palustris Huds.)
… protoplasts. The cauliflower mosaic virus (CaMV) 35S promoter was least active; whereas the rice actin 1 gene 5’ sequence was most active among the six sequences tested. Ranked in order of activity (high to low), the other four 5’ sequences were: 1) the CaMV 35S promoter plus the maize alcohol …
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Secretion of active recombinant phytase from stably transformed soybean cells
… either a constitutive cauliflower mosaic virus 35S promoter or a soybean seed specific β-conglycinin promoter, each with or without a patatin endoplasmic reticulum (ER) signal sequence. All four vectors were sequenced and introduced into 'Williams 82' suspension culture cells by particle …
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Transformation of Brassica napus cv. Westar with the beet western yellows virus coat protein gene
… contains the cauliflower mosaic virus (CaMV) 35S promoter and polyadenylation signal. The CP cDNA was inserted in the plus-sense orientation (clone BW102D) and in the anti-sense orientation (clone BW137D) relative to the CaMV 35S promoter. The CP coding regions of both pRT103 constructs were …
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Effects of Changes of Redox Properties of the Chloroplast ATP Synthase in Arabidopsis
… were overexpressed behind a constitutive 35S promoter compared with the endogenous gamma-subunit gene. In the isolated mutant chloroplasts, the high ATPase activities did not require DTT treatment while in the wild-type plant, ATPase activities increased after DTT treatment indicating that …
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Functional analyses of tomato 3-hydroxy-3-methylglutaryl coenzyme a reductase (HMGR) genes in transgenic plants engineered for altered HMGR expression
… tomato hmg2 in defense responses were studied by promoter analyses of hmg2:GUS gene fusions, overexpression of hmg2 cDNA, and antisense inhibition of hmg1 and hmg2 in transgenic plants. Activity of the hmg2 promoter is developmentally regulated showing expression in seedling cotyledons and …
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Expression of Human Protein C in Transgenic Tobacco
… hPC was fused to an enhanced constitutive 35S promoter (35SDE ) and introduced into a plant transformation vector. The hPC construct was introduced into tobacco leaf disks using Agrobacterium tumefaciens-mediated transformation, and 30 transgenic plants were generated. Stable integration of …
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Characterization and Expression of Plant Defense Genes in Peach and Tomato
… gene, respectively, each driven by the CaMV 35S promoter. Transformation was initially verified by the presence of an nptII gene and GUS enzyme activity in the uidA reporter gene, both engineered into the gene construct. Further confirmation of the transgenics was accomplished by Southern …
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Expression of recombinant porcine preprorelaxin in Nicotiana tabacum
… tabacum, under the control of either the "super" promoter or the dual enhanced cauliflower mosaic virus 35S promoter. Eighteen transgenic tobacco plants were generated that were transformed with the above recombinant genes. Preprorelaxin, mRNA was detected in 12 of the transgenic plants. Fully …
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Expression of atrial natriuretic factor in transgenic potatoes
… by transcriptional fusion with the CaMV 35S promoter. The other two were driven by a 0.7-kb tuber-specific patatin promoter that was isolated by PCR amplification from potato cultivar Maris Piper. Enhancement of expression was attempted by fusing the 5' untranslated leader from the …
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Promoter analysis in transgenic sugar beet
… which encodes P-D-glucuronidase (GUS) to examine promoters in transgenic sugar beet. GUS activities were measured in eight replicates of each of eight sugar beet hairy root clones which had been obtained after inoculation of material grown from a single seed with a CaMV 35S promoted uidA …
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The modification of Anthocyanin biosynthesis in dicotyledonous plants
… different anthocyanin regulatory genes and two promoters. The anthocyanin regulatory genes consisted of a matched pair from maize and a mismatched pair from two dicotyledonous species of plants. In order to accomplish this aim, a series of vectors were made that enabled expression of the genes …
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The modification of Anthocyanin biosynthesis in dicotyledonous plants
… different anthocyanin regulatory genes and two promoters. The anthocyanin regulatory genes consisted of a matched pair from maize and a mismatched pair from two dicotyledonous species of plants. In order to accomplish this aim, a series of vectors were made that enabled expression of the genes …
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Effect of nutritional status on phenotypic characteristics of Arabidopsis and alfalfa in relation to the expression of AtSnRK2.9
… sativa (alfalfa) under the control of the CaMV 35S promoter. Overexpressing D123E Atsnrk2.9 had a serious inhibitory effect on growth and the plants were no longer responsive to changes in phosphate levels. In Arabidopsis, the D123E snrk2.9 overexpressors had a 66% reduction in total seed yield …
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Aphid Transmission, Replication, and Transcription of BYDV -Pav -Il and Genome Organization of RhPV
… driven by Cauliflower mosaic virus-35S promoter (CaMV-35S), were constructed to study the mechanism of replication and transcription of BYDV-PAV-IL in oat protoplasts. Linearized wild-type and mutant cDNA clones express the 22-kDa coat protein and produced genomic and subgenomic RNAs …
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Expression of Nanobodies in Marchantia polymorpha chloroplasts
… Marchantia nuclear genome, I tested different promoters and subcellular locations of protein products. I found that the cauliflower mosaic virus 35S promoter with dual enhancer (<sub>pro</sub>35Sx2) and cytoplasmic localisation of the protein product gave the highest yield of recombinant …
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Identification and Characterization of Genes Involved in Regulation of Ascorbate Metabolic Pathway(s) in Arabidopsis thaliana
… content. Overexpression of AtPAP15 with the CaMV 35S promoter resulted in up to 3-fold higher AsA levels than wt plants, where two independent SALK T-DNA insertion mutants in AtPAP15 had 50% less AsA than wt plants. Enzyme activity of bacterially expressed GST:AtPAP15 was greatest with phytate as …
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The Overexpression of a Feedback -Insensitive Anthranilate Synthase in Arabidopsis and Tobacco
… ASA2 was expressed under the control of the CaMV 35S promoter in Arabidopsis and tobacco to determine the effect of expression of ASA2 on Trp production and Trp-associated secondary metabolism. Transgenic Arabidopsis showed AS activity that was insensitive to inhibition caused by Trp, and had up …
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Structure and Function Analysis of a Plant Transcription Activator
<p>The cauliflower mosaic virus 35S promoter is composed of several cis-elements, each of which confers a different expression pattern in transgenic tobacco. One such cis-element, activation sequence (as)-1 (-83 to -63) mediates preferential expression in root. The element contains a tandem repeat …
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DNA-protein interactions throughout the Arabidopsis calmodulin-3 gene
… The $-$685 to $-$385 region of the calmodulin-3 promoter interacts with abundant A/T-sequence binding nuclear factors, termed high mobility group proteins, which are thought to play an architectural role in regulating the chromatin structure of a gene. Sequence specific DNA-binding proteins that …
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