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Showing 1 to 14 of 14 for “"3-nitrotyrosine"”.
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In vitro selection of deoxyribozymes for O-glycoside cleavage and for 3-nitrotyrosine modification
… the in vitro selection of deoxyribozymes for 3-nitrotyrosine azido-adenylylation described in Chapter 4. Previous research had identified deoxyribozymes for tyrosine azido-adenylylation. The hydroxyl group of 3-nitrotyrosine is many orders of magnitude less reactive than that of tyrosine. In …
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Untersuchungen zur Anbindung von Proteinen und Peptiden an Biomaterialien sowie zur Schädigung dieser Proteine und Peptide durch reaktive Sauerstoff-Spezies
… of reactive molecule species with proteins 3-nitrotyrosine was chosen as a sensitive marker and an assay was established: after pre-column derivatisation with dimethylaminoazobenzenesulfonyl chloride (DABS-Cl) and subsequent analysis via High Performance Liquid Chromatography (HPLC) a …
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Auswirkung von körperlicher Belastung auf die Konzentration von 3-Nitrotyrosin im Atemkondensat
… extracellular lining fluid. The marker 3-nitrotyrosine, which can be detected in breath condensate, helps to examine processes of oxidative stress in the lower airways. At the present time the analysis of breath condensate is not established in the diagnostic routine. This study claims to …
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Three dimensional molecular architectures for the synthesis and improved properties of high performance polymers
… backbones. We also use the detection of 3-nitrotyrosine as a probe to learn more about its physical state in solution. We further utilize the monomer above in a biocompatible post-polymerization functionalization reaction, taking advantage of the polymer's structural motif for the …
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The role of nitric oxide in pre-eclampsia
… lipid peroxides and the peroxynitrite product, 3-nitrotyrosine. An improved GC-MS method was developed to quantify total fatty acids and lipid peroxides. A new reversed phase HPLC technique for the analysis of free 3-nitrotyrosine in human plasma/serum was also developed although sample numbers …
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Mechanistic investigations of the radical initiation pathway of class I ribonucleotide reductase from Escherichia coli
… acid. Thus far, we have replaced Y356 with 3- nitrotyrosine (NO2Y), 2,3-difluorotyrosine (2,3-DFY), 2,3,5-trifluorotyrosine (2,3,5-TFY), and 2,3,5,6-tetrafluorotyrosine (2,3,5,6-TFY). Titrations of the NO2Y-R2 construct allowed us to show that the pKa of the putative residue on the PCET pathway …
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Mechanisms of imporvement following functional inhibition of nitrosative stress after brun and smoke-induced acute lung injury
… measured in the lung parenchyma as well as 3-nitrotyrosine and protein carbonyl formation (indices of RNS and ROS generation, respectively). In our murine study, treatment with the H2S donor significantly reduced the pro- inflammatory cytokine level and increased the anti-inflammatory cytokine …
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Chlorinated Tyrosine Species as Markers of Inflammation: A Kinetic Study
… ring by reactive nitrogen species to produce 3-nitrotyrosine (NO2Tyr) are two such markers. Both are stable byproducts of MPO activity and are readily measurable. Some studies, however, have called into question their use as biomarkers of inflammation. Concentrations of 3ClTyr or NO2Tyr …
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Post-Translational Modification of Proteins via Ambient Air Pollutants and Endogenous Reactive Species
… structure and function. The formation of 3-nitrotyrosine (NTyr) and dityrosine (DiTyr) upon reaction of proteins with ROS/RNS are two common PTMs studied due to their stability and irreversibility, as well as their ability to enhance the allergenicity of pollen allergens upon formation. Many …
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Next generation approaches toward engineering therapeutic proteases
… that selectively hydrolyzes peptides after 3-nitrotyrosine while effectively discriminating against similar peptides containing unmodified tyrosine, sulfotyrosine, phosphotyrosine and phosphoserine. The isolation of protease variants that can discriminate between substrates based on the …
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Natural history and pathogenesis of Takotsubo cardiomyopathy
… looking at the local and systemic rise in the 3-nitrotyrosine (3-NT) as a footmark of ONOO⁻ formation. As a part of clinical investigations, we evaluated the role of RV involvement in early hemodynamic derangement. We performed a meta-analysis to assess the impact of “secondary” TTC, male gender, …
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Bestimmung von 3-Nitrotyrosin im Liquor als Hinweis für nitrosativen Stress
… show the excess of peroxynitrite production, 3-nitrotyrosine (3-NT) was measured, since peroxynitrite itself is a very instable product. 3-NT can be seen as an indicator of nitrosative stress (and peroxynitrite), since it is a stable product out of the reaction of tyrosine und peroxynitrite. …
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CNS Neural/Glial Progenitors as Targets of HIV-1 and Opiates: Effects on Proliferation and Population Dynamics May Alter Behavior Outcomes.
… accompanied by greater microglial activation (3-nitrotyrosine+/Iba-1+). Unbiased stereological estimation in Nissl staining revealed that the depletion of NeuN immunoreactivity in these mice was not due to neuron cell death or loss, because the total neuron number in striatum and total striatal …
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Prediction of concentrations of reactive nitrogen species in aqueous solutions and cells
… ability of GSH and AH- caused 3-nitrotyrosine to be the only tyrosine derivative predicted to be formed at a significant rate. The major GSH reaction product was S-nitrosoglutathione. Analytical (algebraic) expressions were derived for the concentrations of the key reactive …