Global ETD Search
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Showing 1 to 5 of 5 for “"3'-phosphoglycolate"”.
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Action of Tyrosyl DNA Phosphodiesterase on 3'-Phosphoglycolate Terminated DNA Strand Breaks
Free radical-mediated DNA double strand breaks (DSBs) are induced either directly by ionizing radiation or by certain chemicals like bleomycin. These breaks are terminated by 3'-PG (PO4CH2COOˉ) or 3'-phosphate groups formed as a result of fragmentation of deoxyribose. To study the nature of repair …
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Deoxyribose oxidation chemistry and endogenous DNA adducts
… a focus on C4'-oxidation that gives rise to 3'- phosphoglycolate residues on the DNA backbone and releases base propenal or malondialdehyde, and to investigate the role of base propenals in the formation of an important endogenous DNA adduct, MI dG. First, an index of total deoxyribose oxidation …
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Mechanistic studies of bleomycin-mediated double-stranded DNA cleavage and structural studies of DNA containing normal and 4'-oxidized abasic sites
… of the blunt-ended ds-DNA cleavage. A 3'-phosphoglycolate/5'-phosphate (3'PG/5'P) gapped lesion in this "hot spot" has been synthesized to probe the structural basis for re-organization of BLM to the second cleavage site. This lesion was titrated with BLM-Co(III)-OOH. The resulting mixture …
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Nonhomologous end-joining: TDP1-mediated processing, ATM-mediated signaling
… 3'-phosphotyrosyl single-strand breaks and 3'-phosphoglycolate (PG) double-strand breaks (DSBs). Supplementing Tdp1(-/-) extracts with H493R TDP1 partially restored processing of 3'-phosphotyrosyl single-strand breaks, but with evidence of persistent covalent adducts between TDP1 and DNA, …
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ARTEMIS AND METNASE MEDIATED PROCESSING OF 3΄-BLOCKED DNA LESIONS: ROLE IN RADIO/CHEMORESISTANCE AND DNA REPAIR
… mimicking such breaks were constructed. A 3ʹ-phosphoglycolate moiety on longer overhangs (4 and 6 bases) altered specificity and stimulated Metnase-mediated cleavage of the terminal 3 nucleotides. However, an 8-oxoG residue at the single-strand/double-strand border did not affect specificity …