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Showing 1 to 18 of 18 for “"3' end processing"”.
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Interrogating Dux4 Mrna 3′ End Processing
… but contributes to FSHD pathology due to Dux4-dependent cellular reprogramming.</p> <p>We aim to understand what regulatory elements facilitate the cleavage and polyadenylation (CPA) of the Dux4 mRNA beyond the non-consensus PAS and to determine if inefficient CPA underlies the poor expression of …
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Epigenetic Regulation of Replication-Dependent Histone mRNA 3 End Processing
… transkribierten Histon-mRNAs notwendig. In unseren Studien führte ein Knockdown von CDK9 im Zellsystem zu einem ineffizienten Erkennen der korrekten mRNA-Schnittstelle. Infolgedessen setzte RNAPII die Transkription bis zu einem alternativen, genabwärts liegenden …
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Functional Analysis of Drosophila Integrator Complex In Snrna 3' End Processing
… removal of introns from mRNA precursors and the processing of the replication-dependent histone pre-mRNAs. Formation of the 3’ end of these snRNAs is carried out by a poorly characterized, twelve-membered protein complex named Integrator Complex.</p> <p>In the effort to understand Integrator …
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Architecture of eukaryotic mRNA 3' end processing machinery and insights into the mechanism of polyadenylation
… have a polyadenosine (polyA) tail at their 3′ end that is added by a multi-protein complex known as cleavage and polyadenylation factor (CPF). CPF, along with accessory cleavage factors (CF) IA and IB, cleaves the pre-mRNA within the 3' untranslated region (UTR) and adds a poly(A) tail. …
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Mechanistic insights into endoribonuclease activation of the mRNA 3' end processing machinery by Cleavage Factor IA
… must undergo a series of cotranscriptional processing steps for efficient export from the nucleus, translation and mRNA stability. Near the 3' end of the nascent transcript, the pre-mRNA is cleaved at specific RNA sequences, releasing the pre- mRNA from the transcribing RNA polymerase II. …
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In vitro reconstitution of the human pre-mRNA 3′ end processing machinery and mechanistic insights into endonuclease activation
… transcripts in eukaryotes involves several processing steps, including 5′ capping, splicing, and 3′ end processing. The latter entails endonucleolytic cleavage of the nascent pre-mRNA and addition of a poly(A) tail to the resultant free 3′ end. The poly(A) tail then facilitates nuclear …
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An Investigation Into The Role Of Cfia 3' End Processing Complex In The Termination And Initiation/reinitiation Of Transcription
… to examine the function of Clp1, which is an RNA processing factor operating at the 3′ end of genes, in the transcription cycle. Clp1 is one of the four subunits of the CFIA 3′ end processing complex. It is the least investigated CFIA subunit. The role of the other three subunits of the CFIA …
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PolyA signals located near 5’ of genes are silenced by a general mechanism that prevents premature 3’ end processing
PolyA signals located at the 3’ end of eukaryotic genes drive the cleavage and polyadenylation reaction to the nascent pre-mRNA. Although these sequences are expected only at the 3’ end of genes, we found that strong polyA signals are also present within the 5’ untranslated regions (UTRs) of many …
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Structure-Function Analysis of Human Integrator Subunit-4
… and histone mRNA expression. Although the 3′ end formation mechanism and processing machinery of other RNAPII transcripts such as mRNA has been well studied, the mechanism of snRNA 3′ end processing has remained a mystery until the recent discovery of the machinery that mediates this process. …
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General Transcription Factors Play Dual Roles In Initiation And Termination
… initiation factors also interact with the 3' end of a gene. The biological role of initiation factors at the 3' end of a gene is unknown. The general transcription factors TFIIB and TFIIH have been found to interact genetically with Ssu72, a component of CPF 3' end processing complex. …
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A Cell Biological Determination of Integrator Subunit Localization
… that all subunits are required for the proper processing of snRNAs, albeit to differing degrees. Because snRNA transcription takes place in the nucleus of the cell, it is expected that all of the Integrator subunits would exhibit nuclear localization, but the knowledge of discrete subnuclear …
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Functional specialization of Arabidopsis poly(A) polymerases in relation to flowering time and stress
Polyadenylation is a decisive 3’ end processing step during the maturation of pre-mRNAs. The length of the poly(A) tail has an impact on mRNA stability, localization and translatability. Accordingly, many eukaryotic organisms encode several copies of canonical poly(A) polymerases (cPAPs). The …
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Characterisation of the multi-isomeric protein nesprin-1 in p-body and mRNA dynamics
… alternative promoter usage and alternative 3’ end processing, the nesprins are able to generate multiple mRNA transcripts, leading to the production of diverse tissue specific isoforms with potential roles beyond the NE. To explore further the capacity of nesprin-1 to generate alternative …
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Prediction of mRNA polyadenylation sites in the human genome and Mathematical modeling of alternative polyadenylation
… to another are still unknown. This kind of 3 '-end processing can be regulated by the cell environment, cell cycle stage, and tissue type. It is generally accepted that the cleavage of pre-mRNA is based on the sequence of nucleotides around the poly(A) sites. So it is possible to predict the …
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The functions of the RNA polymerase II CTD in transcription and RNA processing
… a means to coordinate transcription and RNA processing- capping, splicing, and 3' end formation. For example, Ser 5, phosphorylated in the start of transcription, promotes the recruitment of capping enzyme, and Ser 2 phosphorylation facilitates RNA 3' end formation and transcription …
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Co-transcriptional recruitment of the U1 snRNP
… introns. Detection of the U1 snRNP is dependent on RNA synthesis and is abolished by intron removal. Microarray data reveals that intron-containing genes are preferentially selected by ChIP with the U1 snRNP furthermore indicating recruitment specificity to introns. Because U1 snRNP levels …
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SR proteins in microRNA/mRNA biogenesis
… such minigene, I have uncovered a splicing-independent function of SF2/ASF in regulating miRNA biogenesis. Directly binding between SF2/ASF protein and pri-miR-7 was demonstrated by Cross-linking and immunoprecipitation assay (CLIP) and RNA affinity purification. The precise binding site was then …
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Investigating nuclear transport proteins as secreted cancer biomarkers
… cells. The aims of this study were to 1) independently validate the MS data showing elevated levels of the nuclear transport proteins in the secretomes of cervical and oesophageal cancer cell lines, 2) investigate the diagnostic potential of members of the nuclear transport protein family using …